CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010689
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 85/88 kDa calcium-independent phospholipase A2 
Protein Synonyms/Alias
 CaI-PLA2; Group VI phospholipase A2; GVI PLA2; Intracellular membrane-associated calcium-independent phospholipase A2 beta; iPLA2-beta; Patatin-like phospholipase domain-containing protein 9; PNPLA9 
Gene Name
 Pla2g6 
Gene Synonyms/Alias
 Pnpla9 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
395LIASKISKLITRKALubiquitination[1]
726AKTVFGAKELGKMVVubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Catalyzes the release of fatty acids from phospholipids. It has been implicated in normal phospholipid remodeling, nitric oxide-induced or vasopressin-induced arachidonic acid release and in leukotriene and prostaglandin production. May participate in fas mediated apoptosis and in regulating transmembrane ion flux in glucose-stimulated B-cells. Has a role in cardiolipin (CL) deacylation. Required for both speed and directionality of monocyte MCP1/CCL2-induced chemotaxis through regulation of F- actin polymerization at the pseudopods (By similarity). 
Sequence Annotation
 REPEAT 151 181 ANK 1.
 REPEAT 185 215 ANK 2.
 REPEAT 219 248 ANK 3.
 REPEAT 251 281 ANK 4.
 REPEAT 286 312 ANK 5.
 REPEAT 316 345 ANK 6.
 REPEAT 349 378 ANK 7.
 DOMAIN 482 666 Patatin.
 REGION 678 697 Calmodulin-binding (By similarity).
 REGION 749 760 Calmodulin-binding (By similarity).
 ACT_SITE 520 520 Potential.
 MOD_RES 588 588 Phosphothreonine.  
Keyword
 Alternative splicing; ANK repeat; Calmodulin-binding; Chemotaxis; Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Lipid degradation; Lipid metabolism; Membrane; Phosphoprotein; Reference proteome; Repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 807 AA 
Protein Sequence
MQFFGRLVNT LSSVTNLFSN PFRVKEVSLT DYVSSERVRE EGQLILLQNV SNRTWDCVLV 60
SPRNPQSGFR LFQLESEADA LVNFQQFSSQ LPPFYESSVQ VLHVEVLQHL TDLIRNHPSW 120
TVTHLAVELG IRECFHHSRI ISCANSTENE EGCTPLHLAC RKGDSEILVE LVQYCHAQMD 180
VTDNKGETAF HYAVQGDNPQ VLQLLGKNAS AGLNQVNNQG LTPLHLACKM GKQEMVRVLL 240
LCNARCNIMG PGGFPIHTAM KFSQKGCAEM IISMDSNQIH SKDPRYGASP LHWAKNAEMA 300
RMLLKRGCDV DSTSSSGNTA LHVAVMRNRF DCVMVLLTYG ANAGARGEHG NTPLHLAMSK 360
DNMEMVKALI VFGAEVDTPN DFGETPALIA SKISKLITRK ALLTLLKTVG ADHHFPIIQG 420
VSTEQGSAAA THPLFSLDRT QPPAISLNNL ELQDLMPISR ARKPAFILSS MRDEKRSHDH 480
LLCLDGGGVK GLVIIQLLIA IEKASGVATK DLFDWVAGTS TGGILALAIL HSKSMAYMRG 540
VYFRMKDEVF RGSRPYESGP LEEFLKREFG EHTKMTDVKK PKVMLTGTLS DRQPAELHLF 600
RNYDAPEAVR EPRCNQNINL KPPTQPADQL VWRAARSSGA APTYFRPNGR FLDGGLLANN 660
PTLDAMTEIH EYNQDMIRKG QGNKVKKLSI VVSLGTGKSP QVPVTCVDVF RPSNPWELAK 720
TVFGAKELGK MVVDCCTDPD GRAVDRARAW CEMVGIQYFR LNPQLGSDIM LDEVSDAVLV 780
NALWETEVYI YEHREEFQKL VQLLLSP 807 
Gene Ontology
 GO:0005813; C:centrosome; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0047499; F:calcium-independent phospholipase A2 activity; IEA:Compara.
 GO:0032049; P:cardiolipin biosynthetic process; IEA:Compara.
 GO:0006935; P:chemotaxis; IEA:UniProtKB-KW.
 GO:0007204; P:elevation of cytosolic calcium ion concentration; IEA:Compara.
 GO:0016042; P:lipid catabolic process; IEA:UniProtKB-KW.
 GO:0060135; P:maternal process involved in female pregnancy; IEA:Compara.
 GO:0007613; P:memory; IEA:Compara.
 GO:0051967; P:negative regulation of synaptic transmission, glutamatergic; IEA:Compara.
 GO:0090238; P:positive regulation of arachidonic acid secretion; IEA:Compara.
 GO:2000304; P:positive regulation of ceramide biosynthetic process; IEA:Compara.
 GO:0045921; P:positive regulation of exocytosis; IEA:Compara.
 GO:0035774; P:positive regulation of insulin secretion involved in cellular response to glucose stimulus; IEA:Compara.
 GO:0090037; P:positive regulation of protein kinase C signaling cascade; IEA:Compara.
 GO:0001934; P:positive regulation of protein phosphorylation; IEA:Compara.
 GO:0090200; P:positive regulation of release of cytochrome c from mitochondria; IEA:Compara.
 GO:0045909; P:positive regulation of vasodilation; IEA:Compara.
 GO:1901339; P:regulation of store-operated calcium channel activity; IEA:Compara.
 GO:0034976; P:response to endoplasmic reticulum stress; IEA:Compara.
 GO:0014832; P:urinary bladder smooth muscle contraction; IEA:Compara. 
Interpro
 IPR016035; Acyl_Trfase/lysoPLipase.
 IPR002110; Ankyrin_rpt.
 IPR020683; Ankyrin_rpt-contain_dom.
 IPR002641; Patatin/PLipase_A2-rel. 
Pfam
 PF00023; Ank
 PF12796; Ank_2
 PF01734; Patatin 
SMART
 SM00248; ANK 
PROSITE
 PS50297; ANK_REP_REGION
 PS50088; ANK_REPEAT 
PRINTS
 PR01415; ANKYRIN.