CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-019235
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable ATP-dependent RNA helicase DDX11 
Protein Synonyms/Alias
 CHL1-related protein 1; hCHLR1; DEAD/H box protein 11; Keratinocyte growth factor-regulated gene 2 protein; KRG-2 
Gene Name
 DDX11 
Gene Synonyms/Alias
 CHL1; CHLR1; KRG2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
38YRVLEAGKIGIFESPubiquitination[1]
335DEALAEVKDMEQLLAubiquitination[1]
345EQLLALGKEARACPYubiquitination[1]
460AVLGGNIKQNPNTQSubiquitination[1]
526FSSREQPKLAGFQQFubiquitination[1]
811RSAELQEKMAYLDQTubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 
Functional Description
 DNA helicase involved in cellular proliferation. Possesses DNA-dependent ATPase and helicase activities. This helicase translocates on single-stranded DNA in the 5' to 3' direction in the presence of ATP and, to a lesser extent, dATP. Its unwinding activity requires a 5'-single-stranded region for helicase loading, since flush-ended duplex structures do not support unwinding. The helicase activity is capable of displacing duplex regions up to 100 bp, which can be extended to 500 bp by RPA or the cohesion establishment factor, the Ctf18-RFC (replication factor C) complex activities. Stimulates the flap endonuclease activity of FEN1. Required for normal sister chromatid cohesion. Required for recruitment of bovine papillomavirus type 1 regulatory protein E2 to mitotic chrmosomes and for viral genome maintenance. Required for maintaining the chromosome segregation and is essential for embryonic development and the prevention of aneuploidy. May function during either S, G2, or M phase of the cell cycle. Binds to both single- and double-stranded DNA. 
Sequence Annotation
 DOMAIN 9 445 Helicase ATP-binding.
 NP_BIND 44 51 ATP (By similarity).
 MOTIF 393 396 DEAH.
 METAL 267 267 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 285 285 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 315 315 Iron-sulfur (4Fe-4S) (By similarity).
 METAL 350 350 Iron-sulfur (4Fe-4S) (By similarity).  
Keyword
 4Fe-4S; Alternative splicing; ATP-binding; Cell cycle; Complete proteome; Disease mutation; DNA-binding; Helicase; Host-virus interaction; Hydrolase; Iron; Iron-sulfur; Metal-binding; Nucleotide-binding; Nucleus; Polymorphism; Reference proteome; RNA-binding. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 970 AA 
Protein Sequence
MANETQKVGA IHFPFPFTPY SIQEDFMAEL YRVLEAGKIG IFESPTGTGK SLSLICGALS 60
WLRDFEQKKR EEEARLLETG TGPLHDEKDE SLCLSSSCEG AAGTPRPAGE PAWVTQFVQK 120
KEERDLVDRL KAEQARRKQR EERLQQLQHR VQLKYAAKRL RQEEEERENL LRLSREMLET 180
GPEAERLEQL ESGEEELVLA EYESDEEKKV ASRVDEDEDD LEEEHITKIY YCSRTHSQLA 240
QFVHEVKKSP FGKDVRLVSL GSRQNLCVNE DVKSLGSVQL INDRCVDMQR SRHEKKKGAE 300
EEKPKRRRQE KQAACPFYNH EQMGLLRDEA LAEVKDMEQL LALGKEARAC PYYGSRLAIP 360
AAQLVVLPYQ MLLHAATRQA AGIRLQDQVV IIDEAHNLID TITGMHSVEV SGSQLCQAHS 420
QLLQYVERYG KRLKAKNLMY LKQILYLLEK FVAVLGGNIK QNPNTQSLSQ TGTELKTIND 480
FLFQSQIDNI NLFKVQRYCE KSMISRKLFG FTERYGAVFS SREQPKLAGF QQFLQSLQPR 540
TTEALAAPAD ESQASTLRPA SPLMHIQGFL AALTTANQDG RVILSRQGSL SQSTLKFLLL 600
NPAVHFAQVV KECRAVVIAG GTMQPVSDFR QQLLACAGVE AERVVEFSCG HVIPPDNILP 660
LVICSGISNQ PLEFTFQKRE LPQMMDEVGR ILCNLCGVVP GGVVCFFPSY EYLRQVHAHW 720
EKGGLLGRLA ARKKIFQEPK SAHQVEQVLL AYSRCIQACG QERGQVTGAL LLSVVGGKMS 780
EGINFSDNLG RCVVMVGMPF PNIRSAELQE KMAYLDQTLS PRPGTPREGS GGEPVHEGRQ 840
PVHRQGHQAP EGFCQRSAPG PAICPAPCPG QAAGLDPSPC GGQSYLWPRH CCCAEVSPGE 900
VGLFLMGNHT TAWRRALPLS CPLETVFVVG VVCGDPVTKV KPRRRVWSPE CCQDPGTGVS 960
SRRRKWGNPE 970 
Gene Ontology
 GO:0030496; C:midbody; IDA:UniProtKB.
 GO:0000790; C:nuclear chromatin; IDA:UniProtKB.
 GO:0005730; C:nucleolus; TAS:ProtInc.
 GO:0005654; C:nucleoplasm; TAS:Reactome.
 GO:0000922; C:spindle pole; IDA:UniProtKB.
 GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004003; F:ATP-dependent DNA helicase activity; IEA:InterPro.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0003678; F:DNA helicase activity; TAS:ProtInc.
 GO:0008094; F:DNA-dependent ATPase activity; IDA:UniProtKB.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
 GO:0006987; P:activation of signaling protein activity involved in unfolded protein response; TAS:Reactome.
 GO:0044267; P:cellular protein metabolic process; TAS:Reactome.
 GO:0000086; P:G2/M transition of mitotic cell cycle; TAS:ProtInc.
 GO:0000070; P:mitotic sister chromatid segregation; TAS:ProtInc.
 GO:0008284; P:positive regulation of cell proliferation; TAS:ProtInc.
 GO:0000084; P:S phase of mitotic cell cycle; TAS:ProtInc.
 GO:0007062; P:sister chromatid cohesion; IDA:UniProtKB.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 
Interpro
 IPR006555; ATP-dep_Helicase_C.
 IPR027076; DDX11/CHL1.
 IPR010614; DEAD_2.
 IPR013020; DNA_helicase_DNA-repair_Rad3.
 IPR014013; Helic_SF1/SF2_ATP-bd_DinG/Rad3.
 IPR006554; Helicase-like_DEXD_c2.
 IPR027417; P-loop_NTPase. 
Pfam
 PF06733; DEAD_2
 PF13307; Helicase_C_2 
SMART
 SM00488; DEXDc2
 SM00491; HELICc2 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51193; HELICASE_ATP_BIND_2 
PRINTS