UniProt Accession

 ACLY_MOUSE; Q91V92 

Genbank Protein ID

 AF332052; AF332051; BC056378 

Genbank Nucleotide ID

 AAK56081.1; AAK56080.1; AAH56378.1 

Protein Name

 ATP-citrate synthase 

Protein Synonyms/Alias

 ATP-citrate (pro-S-)-lyase; Citrate cleavage enzyme 

Gene Name

 Acly 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
148	DVGDVDAKAQKLLVG	ubiquitination	[1]
151	DVDAKAQKLLVGVDE	ubiquitination	[1]
159	LLVGVDEKLNTEDIK	ubiquitination	[1]
471	VAPAKKAKPAMPQGK	ubiquitination	[1]
478	KPAMPQGKSATLFSR	acetylation	[2]
478	KPAMPQGKSATLFSR	ubiquitination	[1]
1067	IGHYLDQKRLKQGLY	ubiquitination	[1]
1070	YLDQKRLKQGLYRHP	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337] 

Functional Description

 ATP citrate-lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. Has a central role in de novo lipid synthesis. In nervous tissue it may be involved in the biosynthesis of acetylcholine (By similarity). 

Sequence Annotation

 NP_BIND 691 711 ATP (By similarity).
 NP_BIND 742 768 ATP (By similarity).
 REGION 769 779 CoA-binding (Potential).
 ACT_SITE 750 750 Tele-phosphohistidine intermediate (By
 METAL 708 708 Magnesium (By similarity).
 BINDING 346 346 Citrate; via amide nitrogen (By
 BINDING 348 348 Citrate (By similarity).
 BINDING 379 379 Citrate (By similarity).
 MOD_RES 131 131 Phosphotyrosine (By similarity).
 MOD_RES 447 447 Phosphothreonine (By similarity).
 MOD_RES 451 451 Phosphoserine (By similarity).
 MOD_RES 455 455 Phosphoserine; by PKA and PKB/AKT1 or
 MOD_RES 536 536 N6-acetyllysine (By similarity).
 MOD_RES 544 544 N6-acetyllysine (By similarity).
 MOD_RES 629 629 Phosphothreonine (By similarity).
 MOD_RES 653 653 Phosphoserine (By similarity).
 MOD_RES 672 672 Phosphotyrosine.
 MOD_RES 829 829 Phosphoserine (By similarity).
 MOD_RES 938 938 N6-acetyllysine (By similarity).
 MOD_RES 958 958 N6-acetyllysine (By similarity).
 MOD_RES 1067 1067 N6-acetyllysine (By similarity).
 MOD_RES 1090 1090 Phosphoserine (By similarity).  

Keyword

 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Lipid biosynthesis; Lipid metabolism; Magnesium; Metal-binding; Nucleotide-binding; Phosphoprotein; Reference proteome; Transferase; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 1091 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0003878; F:ATP citrate synthase activity; TAS:MGI.
 GO:0048037; F:cofactor binding; IEA:InterPro.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004775; F:succinate-CoA ligase (ADP-forming) activity; IEA:InterPro.
 GO:0006085; P:acetyl-CoA biosynthetic process; TAS:MGI.
 GO:0044262; P:cellular carbohydrate metabolic process; IEA:InterPro.
 GO:0006629; P:lipid metabolic process; IEA:UniProtKB-KW. 

Interpro

 IPR014608; ATP-citrate_synthase.
 IPR013650; ATP-grasp_succ-CoA_synth-type.
 IPR013816; ATP_grasp_subdomain_2.
 IPR017440; Cit_synth/succinyl-CoA_lig_AS.
 IPR016143; Citrate_synth-like_sm_a-sub.
 IPR002020; Citrate_synthase-like.
 IPR016141; Citrate_synthase-like_core.
 IPR003781; CoA-bd.
 IPR005810; CoA_lig_alpha.
 IPR005811; CoA_ligase.
 IPR016040; NAD(P)-bd_dom.
 IPR017866; Succ-CoA_synthase_bsu_CS.
 IPR016102; Succinyl-CoA_synth-like. 

Pfam

 PF08442; ATP-grasp_2
 PF00285; Citrate_synt
 PF02629; CoA_binding
 PF00549; Ligase_CoA 

SMART

  

PROSITE

 PS01216; SUCCINYL_COA_LIG_1
 PS00399; SUCCINYL_COA_LIG_2
 PS01217; SUCCINYL_COA_LIG_3 

PRINTS