UniProt Accession

 MAAI_HUMAN; O43708; A6NED0; A6NNB8; A8MWD7; B2RCK3; O15308; O75430; Q6IB17; Q7Z610; Q9BV63 

Genbank Protein ID

 AJ001838; U86529; AF053545; AF053539; AF053540; AF053541; AF053542; AF053543; AF053544; AF098318; AF095582; AF098311; AF098312; AF098313; AF098314; AF098315; AF098316; AF098317; AK315154; CR456987; AY316305; AC007954; CH471061; CH471061; BC001453 

Genbank Nucleotide ID

 CAA05045.1; AAB96392.1; AAC33591.1; AAC33591.1; AAC33591.1; AAC33591.1; AAC33591.1; AAC33591.1; AAC33591.1; AAD43007.1; AAD43007.1; AAD43007.1; AAD43007.1; AAD43007.1; AAD43007.1; AAD43007.1; AAD43007.1; AAD43007.1; BAG37600.1; CAG33268.1; AAP69526.1; AAF62559.1; EAW81278.1; EAW81279.1; AAH01453.1 

Protein Name

 Maleylacetoacetate isomerase 

Protein Synonyms/Alias

 MAAI; GSTZ1-1; Glutathione S-transferase zeta 1 

Gene Name

 GSTZ1 

Gene Synonyms/Alias

 MAAI 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
32	ALKGIDYKTVPINLI	acetylation	[1, 2, 3]

Reference

 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Proteomic investigations reveal a role for RNA processing factor THRAP3 in the DNA damage response.
 Beli P, Lukashchuk N, Wagner SA, Weinert BT, Olsen JV, Baskcomb L, Mann M, Jackson SP, Choudhary C.
 Mol Cell. 2012 Apr 27;46(2):212-25. [PMID: 22424773]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 

Functional Description

 Bifunctional enzyme showing minimal glutathione- conjugating activity with ethacrynic acid and 7-chloro-4- nitrobenz-2-oxa-1,3-diazole and maleylacetoacetate isomerase activity. Has also low glutathione peroxidase activity with T- butyl and cumene hydroperoxides. Is able to catalyze the glutathione dependent oxygenation of dichloroacetic acid to glyoxylic acid. 

Sequence Annotation

 DOMAIN 4 87 GST N-terminal.
 DOMAIN 92 212 GST C-terminal.
 REGION 14 19 Glutathione binding.
 REGION 71 72 Glutathione binding.
 REGION 115 117 Glutathione binding.
 BINDING 45 45 Glutathione.
 BINDING 59 59 Glutathione; via amide nitrogen and
 BINDING 111 111 Glutathione.
 MOD_RES 32 32 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Alternative splicing; Complete proteome; Cytoplasm; Isomerase; Multifunctional enzyme; Phenylalanine catabolism; Polymorphism; Reference proteome; Transferase; Tyrosine catabolism. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 216 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005739; C:mitochondrion; ISS:UniProtKB.
 GO:0004602; F:glutathione peroxidase activity; TAS:ProtInc.
 GO:0004364; F:glutathione transferase activity; IDA:UniProtKB.
 GO:0016034; F:maleylacetoacetate isomerase activity; IDA:UniProtKB.
 GO:0006749; P:glutathione metabolic process; IDA:UniProtKB.
 GO:0006559; P:L-phenylalanine catabolic process; TAS:Reactome.
 GO:0006572; P:tyrosine catabolic process; IEA:UniProtKB-KW. 

Interpro

 IPR010987; Glutathione-S-Trfase_C-like.
 IPR004045; Glutathione_S-Trfase_N.
 IPR017933; Glutathione_S_Trfase/Cl_chnl_C.
 IPR004046; GST_C.
 IPR005955; Mal_ac_isom.
 IPR012336; Thioredoxin-like_fold. 

Pfam

 PF00043; GST_C 

SMART

  

PROSITE

 PS50405; GST_CTER
 PS50404; GST_NTER 

PRINTS