UniProt Accession

 ARP4_YEAST; P80428; D6VWA2 

Genbank Protein ID

 X75317; X83502; Z49356; BK006943 

Genbank Nucleotide ID

 CAA53066.1; CAA58489.1; CAA89374.1; DAA08718.1 

Protein Name

 Actin-related protein 4 

Protein Synonyms/Alias

 Actin-like protein ARP4; Actin-like protein 4 

Gene Name

 ARP4 

Gene Synonyms/Alias

 ACT3; YJL081C; J1012 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
323	RNDYVPLKRTKPSGV	acetylation	[1]
350	KEQEAVSKSTSPAAN	acetylation	[2]

Reference

 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591] 

Functional Description

 Chromatin interaction component of the NuA4 histone acetyltransferase complex which is involved in transcriptional activation of selected genes principally by acetylation of nucleosomal histone H4 and H2A. The NuA4 complex is also involved in DNA repair. ARP4 recognizes H2AS128ph (gamma-H2A) and is required for NuA4 complex integrity. Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Component of the INO80 complex which remodels chromatin by shifting nucleosomes. Its ability to induce transcription of some phosphate-responsive genes is modulated by inositol polyphosphates. The INO80 complex is involved in DNA repair by associating to gamma-H2A as a response to DNA damage. 

Sequence Annotation

 MOD_RES 349 349 Phosphoserine.  

Keyword

 3D-structure; Activator; Chromatin regulator; Complete proteome; Direct protein sequencing; DNA damage; DNA repair; Nucleus; Phosphoprotein; Reference proteome; Repeat; Transcription; Transcription regulation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 489 AA 

Protein Sequence

Gene Ontology

 GO:0031011; C:Ino80 complex; IPI:SGD.
 GO:0035267; C:NuA4 histone acetyltransferase complex; IPI:SGD.
 GO:0000812; C:Swr1 complex; IDA:SGD.
 GO:0003682; F:chromatin binding; IDA:SGD.
 GO:0006338; P:chromatin remodeling; IDA:SGD.
 GO:0006281; P:DNA repair; IDA:SGD.
 GO:0016573; P:histone acetylation; IDA:SGD.
 GO:0051382; P:kinetochore assembly; IMP:SGD.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:SGD.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 

Interpro

 IPR004000; Actin-related.
 IPR020902; Actin/actin-like_CS. 

Pfam

 PF00022; Actin 

SMART

 SM00268; ACTIN 

PROSITE

 PS00406; ACTINS_1
 PS00432; ACTINS_2
 PS01132; ACTINS_ACT_LIKE 

PRINTS