UniProt Accession

 GSTK1_MOUSE; Q9DCM2 

Genbank Protein ID

 AK002661; AY279096; BC058163 

Genbank Nucleotide ID

 BAB22268.1; AAP20655.1; AAH58163.1 

Protein Name

 Glutathione S-transferase kappa 1 

Protein Synonyms/Alias

 GST 13-13; GST class-kappa; GSTK1-1; mGSTK1; Glutathione S-transferase subunit 13 

Gene Name

 Gstk1 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
36	YQHLWNIKLQLRPTL	acetylation	[1]
36	YQHLWNIKLQLRPTL	succinylation	[1]
49	TLIAGIMKDSGNQPP	acetylation	[1, 2, 3, 4]
49	TLIAGIMKDSGNQPP	succinylation	[1]
68	RKGQYIFKEIPLLKQ	acetylation	[1, 2, 3, 4, 5, 6, 7]
68	RKGQYIFKEIPLLKQ	succinylation	[1]
68	RKGQYIFKEIPLLKQ	ubiquitination	[8]
74	FKEIPLLKQFFQVPL	acetylation	[1, 3, 4, 6, 7]
74	FKEIPLLKQFFQVPL	succinylation	[1]
74	FKEIPLLKQFFQVPL	ubiquitination	[8]
85	QVPLNIPKDFFGETV	acetylation	[3, 4]
85	QVPLNIPKDFFGETV	ubiquitination	[8]
93	DFFGETVKKGSINAM	acetylation	[1, 2, 3, 4, 7, 9]
93	DFFGETVKKGSINAM	succinylation	[1]
93	DFFGETVKKGSINAM	ubiquitination	[8]
116	EQPEMLEKVSREIWM	acetylation	[1, 2, 3, 4, 7]
116	EQPEMLEKVSREIWM	succinylation	[1]
116	EQPEMLEKVSREIWM	ubiquitination	[8]
144	SILAAAVKAGMSTAQ	acetylation	[1]
144	SILAAAVKAGMSTAQ	succinylation	[1]
158	QAQHFLEKISTQQVK	acetylation	[1, 2, 3, 4, 6, 7, 10]
158	QAQHFLEKISTQQVK	succinylation	[1]
165	KISTQQVKNKLIENT	acetylation	[1, 2, 3, 4]
165	KISTQQVKNKLIENT	succinylation	[1]
167	STQQVKNKLIENTDA	acetylation	[1, 2, 3, 4, 7, 10]
167	STQQVKNKLIENTDA	succinylation	[1]
167	STQQVKNKLIENTDA	ubiquitination	[8]
177	ENTDAACKYGAFGLP	acetylation	[1, 2, 3, 4, 6, 7, 10]
177	ENTDAACKYGAFGLP	succinylation	[1]
193	TVAHVDGKTYMLFGS	acetylation	[1, 2, 4]
193	TVAHVDGKTYMLFGS	succinylation	[1]

Reference

 [1] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [5] The fasted/fed mouse metabolic acetylome: N6-acetylation differences suggest acetylation coordinates organ-specific fuel switching.
 Yang L, Vaitheesvaran B, Hartil K, Robinson AJ, Hoopmann MR, Eng JK, Kurland IJ, Bruce JE.
 J Proteome Res. 2011 Sep 2;10(9):4134-49. [PMID: 21728379]
 [6] Mitochondrial acetylome analysis in a mouse model of alcohol-induced liver injury utilizing SIRT3 knockout mice.
 Fritz KS, Galligan JJ, Hirschey MD, Verdin E, Petersen DR.
 J Proteome Res. 2012 Mar 2;11(3):1633-43. [PMID: 22309199]
 [7] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [8] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [9] Substrate and functional diversity of lysine acetylation revealed by a proteomics survey.
 Kim SC, Sprung R, Chen Y, Xu Y, Ball H, Pei J, Cheng T, Kho Y, Xiao H, Xiao L, Grishin NV, White M, Yang XJ, Zhao Y.
 Mol Cell. 2006 Aug;23(4):607-18. [PMID: 16916647]
 [10] Circadian acetylome reveals regulation of mitochondrial metabolic pathways.
 Masri S, Patel VR, Eckel-Mahan KL, Peleg S, Forne I, Ladurner AG, Baldi P, Imhof A, Sassone-Corsi P.
 Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3339-44. [PMID: 23341599] 

Functional Description

  

Sequence Annotation

 REGION 16 18 Glutathione binding (By similarity).
 REGION 200 201 Glutathione binding (By similarity).
 BINDING 53 53 Glutathione (By similarity).
 BINDING 183 183 Glutathione; via amide nitrogen and
 MOD_RES 93 93 N6-acetyllysine.  

Keyword

 Acetylation; Complete proteome; Direct protein sequencing; Mitochondrion; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 226 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005759; C:mitochondrial matrix; IEA:Compara.
 GO:0005777; C:peroxisome; IEA:Compara.
 GO:0004602; F:glutathione peroxidase activity; IDA:MGI.
 GO:0004364; F:glutathione transferase activity; IDA:MGI.
 GO:0015035; F:protein disulfide oxidoreductase activity; IEA:InterPro.
 GO:0006749; P:glutathione metabolic process; IDA:MGI. 

Interpro

 IPR001853; DSBA-like_thioredoxin_dom.
 IPR014440; HCCAis_GSTk.
 IPR012336; Thioredoxin-like_fold. 

Pfam

 PF01323; DSBA 

SMART

  

PROSITE

  

PRINTS