| Tag | Content |
|---|
| CPLM ID | CPLM-006718 |
UniProt Accession | |
Genbank Protein ID | |
Genbank Nucleotide ID | |
Protein Name | Phosphoenolpyruvate-protein phosphotransferase PtsP |
Protein Synonyms/Alias | Enzyme I-Ntr; Phosphotransferase system, enzyme I |
Gene Name | ptsP |
Gene Synonyms/Alias | ygdF; ygdO; b2829; JW2797 |
Created Date | July 27, 2013 |
Organism | Escherichia coli (strain K12) |
NCBI Taxa ID | 83333 |
Lysine Modification | Position | Peptide | Type | References |
|---|
| 92 | INLADAQKHPSFKYI | acetylation | [1] | | 97 | AQKHPSFKYIPSVKE | acetylation | [1] | | 436 | LKSGERIKVMLNAGL | acetylation | [1] | | 450 | LSPEHEEKLGSRIDG | acetylation | [1] |
|
Reference | [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli. Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C. Mol Cell. 2013 Jul 25;51(2):265-72. [ PMID: 23830618] |
Functional Description | Component of the phosphoenolpyruvate-dependent nitrogen- metabolic phosphotransferase system (nitrogen-metabolic PTS), that seems to be involved in regulating nitrogen metabolism. Enzyme I- Ntr transfers the phosphoryl group from phosphoenolpyruvate (PEP) to the phosphoryl carrier protein (NPr). Could function in the transcriptional regulation of sigma-54 dependent operons in conjunction with the NPr (PtsO) and EIIA-Ntr (PtsN) proteins. |
Sequence Annotation | DOMAIN 1 127 GAF.
REGION 128 170 Linker.
REGION 171 748 PTS EI.
ACT_SITE 356 356 Tele-phosphohistidine intermediate (By
ACT_SITE 668 668 Proton donor (By similarity).
METAL 597 597 Magnesium (By similarity).
METAL 621 621 Magnesium (By similarity).
BINDING 462 462 Substrate (By similarity).
BINDING 498 498 Substrate (By similarity).
BINDING 597 597 Substrate (By similarity).
BINDING 618 618 Substrate; via carbonyl oxygen (By
BINDING 619 619 Substrate; via amide nitrogen (By
BINDING 620 620 Substrate (By similarity).
BINDING 621 621 Substrate; via amide nitrogen (By |
Keyword | Complete proteome; Cytoplasm; Kinase; Magnesium; Metal-binding; Phosphotransferase system; Reference proteome; Sugar transport; Transferase; Transport. |
Sequence Source | UniProt (SWISSPROT/TrEMBL); GenBank; EMBL |
Protein Length | 748 AA |
Protein Sequence | MLTRLREIVE KVASAPRLNE ALNILVTDIC LAMDTEVCSV YLADHDRRCY YLMATRGLKK 60
PRGRTVTLAF DEGIVGLVGR LAEPINLADA QKHPSFKYIP SVKEERFRAF LGVPIIQRRQ 120
LLGVLVVQQR ELRQYDESEE SFLVTLATQM AAILSQSQLT ALFGQYRQTR IRALPAAPGV 180
AIAEGWQDAT LPLMEQVYQA STLDPALERE RLTGALEEAA NEFRRYSKRF AAGAQKETAA 240
IFDLYSHLLS DTRLRRELFA EVDKGSVAEW AVKTVIEKFA EQFAALSDNY LKERAGDLRA 300
LGQRLLFHLD DANQGPNAWP ERFILVADEL SATTLAELPQ DRLVGVVVRD GAANSHAAIM 360
VRALGIPTVM GADIQPSVLH RRTLIVDGYR GELLVDPEPV LLQEYQRLIS EEIELSRLAE 420
DDVNLPAQLK SGERIKVMLN AGLSPEHEEK LGSRIDGIGL YRTEIPFMLQ SGFPSEEEQV 480
AQYQGMLQMF NDKPVTLRTL DVGADKQLPY MPISEENPCL GWRGIRITLD QPEIFLIQVR 540
AMLRANAATG NLNILLPMVT SLDEVDEARR LIERAGREVE EMIGYEIPKP RIGIMLEVPS 600
MVFMLPHLAK RVDFISVGTN DLTQYILAVD RNNTRVANIY DSLHPAMLRA LAMIAREAEI 660
HGIDLRLCGE MAGDPMCVAI LIGLGYRHLS MNGRSVARAK YLLRRIDYAE AENLAQRSLE 720
AQLATEVRHQ VAAFMERRGM GGLIRGGL 748 |
Gene Ontology | GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell. GO:0016301; F:kinase activity; IEA:UniProtKB-KW. GO:0046872; F:metal ion binding; IEA:UniProtKB-KW. GO:0008965; F:phosphoenolpyruvate-protein phosphotransferase activity; IEA:EC. GO:0005351; F:sugar:hydrogen symporter activity; IEA:InterPro. GO:0009401; P:phosphoenolpyruvate-dependent sugar phosphotransferase system; IEA:UniProtKB-KW. |
Interpro | |
Pfam | |
SMART | |
PROSITE | |
PRINTS | |