CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-012627
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable E3 ubiquitin-protein ligase HERC1 
Protein Synonyms/Alias
 HECT domain and RCC1-like domain-containing protein 1; p532; p619 
Gene Name
 HERC1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
58PQQVLCLKGPQLPDFubiquitination[1]
448NNQSTLKKLTFEPHRubiquitination[1]
1291ESRYQPGKHLSEVYRubiquitination[1]
1311RSRLLACKNLELIQTubiquitination[1]
2014KEKEQEIKLQKQGELubiquitination[1]
2339ATLLGVVKEGSTSAKubiquitination[1]
2769FSLRQIAKAMEATGAubiquitination[1, 2, 3, 4]
2881SAVTRRHKFDLAARTubiquitination[1, 2, 3, 4, 5]
3042LCGTCREKYLAMKTKubiquitination[1]
3059STSSERYKGQAPDLIubiquitination[4]
3311VDDSIQRKFLPSFLRubiquitination[1, 2, 3]
3348LLADKGAKLRPNYDKubiquitination[1]
3355KLRPNYDKSEVEKKGubiquitination[1]
3460RVWNVTKKQYSLQQTubiquitination[1, 2, 3]
3837ATCRTALKQQGVLGLubiquitination[1, 3]
4462PMVRSIGKTMVQGKNubiquitination[1, 4]
4468GKTMVQGKNYGPQITubiquitination[1, 2, 4]
4487STRGRKCKPIFVQIAubiquitination[1]
4499QIARQVVKLNASDLRubiquitination[1]
4797SQRFQIMKVDRPYDSubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724
Functional Description
 Involved in membrane trafficking via some guanine nucleotide exchange factor (GEF) activity and its ability to bind clathrin. Acts as a GEF for Arf and Rab, by exchanging bound GDP for free GTP. Binds phosphatidylinositol 4,5-bisphosphate, which is required for GEF activity. May also act as a E3 ubiquitin- protein ligase which accepts ubiquitin from an E2 ubiquitin- conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. 
Sequence Annotation
 REPEAT 371 420 RCC1 1.
 REPEAT 421 475 RCC1 2.
 REPEAT 441 483 WD 1.
 REPEAT 476 528 RCC1 3.
 REPEAT 506 545 WD 2.
 REPEAT 529 578 RCC1 4.
 REPEAT 580 631 RCC1 5.
 REPEAT 633 682 RCC1 6.
 REPEAT 660 699 WD 3.
 REPEAT 683 735 RCC1 7.
 DOMAIN 2002 2193 B30.2/SPRY.
 REPEAT 3426 3465 WD 4.
 REPEAT 3484 3522 WD 5.
 REPEAT 3524 3572 WD 6.
 REPEAT 3580 3619 WD 7.
 REPEAT 3624 3663 WD 8.
 REPEAT 3667 3713 WD 9.
 REPEAT 3745 3784 WD 10.
 REPEAT 3996 4044 RCC1 8.
 REPEAT 4046 4099 RCC1 9.
 REPEAT 4101 4151 RCC1 10.
 REPEAT 4153 4203 RCC1 11.
 REPEAT 4205 4256 RCC1 12.
 REPEAT 4258 4308 RCC1 13.
 REPEAT 4287 4325 WD 11.
 REPEAT 4310 4360 RCC1 14.
 DOMAIN 4501 4848 HECT.
 ACT_SITE 4811 4811 Glycyl thioester intermediate (By
 MOD_RES 1491 1491 Phosphoserine.
 MOD_RES 1517 1517 Phosphoserine.
 MOD_RES 1521 1521 Phosphoserine.
 MOD_RES 2701 2701 Phosphothreonine.
 MOD_RES 2706 2706 Phosphoserine.
 MOD_RES 2710 2710 Phosphoserine.
 MOD_RES 4857 4857 Phosphoserine.  
Keyword
 Complete proteome; Cytoplasm; Golgi apparatus; Guanine-nucleotide releasing factor; Ligase; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Transport; Ubl conjugation pathway; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 4861 AA 
Protein Sequence
MATMIPPVKL KWLEHLNSSW ITEDSESIAT REGVAVLYSK LVSNKEVVPL PQQVLCLKGP 60
QLPDFERESL SSDEQDHYLD ALLSSQLALA KMVCSDSPFA GALRKRLLVL QRVFYALSNK 120
YHDKGKVKQQ QHSPESSSGS ADVHSVSERP RSSTDALIEM GVRTGLSLLF ALLRQSWMMP 180
VSGPGLSLCN DVIHTAIEVV SSLPPLSLAN ESKIPPMGLD CLSQVTTFLK GVTIPNSGAD 240
TLGRRLASEL LLGLAAQRGS LRYLLEWIEM ALGASAVVHT MEKGKLLSSQ EGMISFDCFM 300
TILMQMRRSL GSSADRSQWR EPTRTSDGLC SLYEAALCLF EEVCRMASDY SRTCASPDSI 360
QTGDAPIVSE TCEVYVWGSN SSHQLVEGTQ EKILQPKLAP SFSDAQTIEA GQYCTFVIST 420
DGSVRACGKG SYGRLGLGDS NNQSTLKKLT FEPHRSIKKV SSSKGSDGHT LAFTTEGEVF 480
SWGDGDYGKL GHGNSSTQKY PKLIQGPLQG KVVVCVSAGY RHSAAVTEDG ELYTWGEGDF 540
GRLGHGDSNS RNIPTLVKDI SNVGEVSCGS SHTIALSKDG RTVWSFGGGD NGKLGHGDTN 600
RVYKPKVIEA LQGMFIRKVC AGSQSSLALT STGQVYAWGC GACLGCGSSE ATALRPKLIE 660
ELAATRIVDV SIGDSHCLAL SHDNEVYAWG NNSMGQCGQG NSTGPITKPK KVSGLDGIAI 720
QQISAGTSHS LAWTALPRDR QVVAWHRPYC VDLEESTFSH LRSFLERYCD KINSEIPPLP 780
FPSSREHHSF LKLCLKLLSN HLALALAGGV ATSILGRQAG PLRNLLFRLM DSTVPDEIQE 840
VVIETLSVGA TMLLPPLRER MELLHSLLPQ GPDRWESLSK GQRMQLDIIL TSLQDHTHVA 900
SLLGYSSPSD AADLSSVCTG YGNLSDQPYG TQSCHPDTHL AEILMKTLLR NLGFYTDQAF 960
GELEKNSDKF LLGTSSSENS QPAHLHELLC SLQKQLLAFC HINNISENSS SVALLHKHLQ 1020
LLLPHATDIY SRSANLLKES PWNGSVGEKL RDVIYVSAAG SMLCQIVNSL LLLPVSVARP 1080
LLSYLLDLLP PLDCLNRLLP AADLLEDQEL QWPLHGGPEL IDPAGLPLPQ PAQSWVWLVD 1140
LERTIALLIG RCLGGMLQGS PVSPEEQDTA YWMKTPLFSD GVEMDTPQLD KCMSCLLEVA 1200
LSGNEEQKPF DYKLRPEIAV YVDLALGCSK EPARSLWISM QDYAVSKDWD SATLSNESLL 1260
DTVSRFVLAA LLKHTNLLSQ ACGESRYQPG KHLSEVYRCV YKVRSRLLAC KNLELIQTRS 1320
SSRDRWISEN QDSADVDPQE HSFTRTIDEE AEMEEQAERD REEGHPEPED EEEEREHEVM 1380
TAGKIFQCFL SAREVARSRD RDRMNSGAGS GARADDPPPQ SQQERRVSTD LPEGQDVYTA 1440
ACNSVIHRCA LLILGVSPVI DELQKRREEG QLQQPSTSAS EGGGLMTRSE SLTAESRLVH 1500
TSPNYRLIKS RSESDLSQPE SDEEGYALSG RRNVDLDLAA SHRKRGPMHS QLESLSDSWA 1560
RLKHSRDWLC NSSYSFESDF DLTKSLGVHT LIENVVSFVS GDVGNAPGFK EPEESMSTSP 1620
QASIIAMEQQ QLRAELRLEA LHQILVLLSG MEEKGSISLA GSRLSSGFQS STLLTSVRLQ 1680
FLAGCFGLGT VGHTGGKGES GRLHHYQDGI RAAKRNIQIE IQVAVHKIYQ QLSATLERAL 1740
QANKHHIEAQ QRLLLVTVFA LSVHYQPVDV SLAISTGLLN VLSQLCGTDT MLGQPLQLLP 1800
KTGVSQLSTA LKVASTRLLQ ILAITTGTYA DKLSPKVVQS LLDLLCSQLK NLLSQTGVLH 1860
MASFGEGEQE DGEEEEKKVD SSGETEKKDF RAALRKQHAA ELHLGDFLVF LRRVVSSKAI 1920
QSKMASPKWT EVLLNIASQK CSSGIPLVGN LRTRLLALHV LEAVLPACES GVEDDQMAQI 1980
VERLFSLLSD CMWETPIAQA KHAIQIKEKE QEIKLQKQGE LEEEDENLPI QEVSFDPEKA 2040
QCCLVENGQI LTHGSGGKGY GLASTGVTSG CYQWKFYIVK ENRGNEGTCV GVSRWPVHDF 2100
NHRTTSDMWL YRAYSGNLYH NGEQTLTLSS FTQGDFITCV LDMEARTISF GKNGEEPKLA 2160
FEDVDAAELY PCVMFYSSNP GEKVKICDMQ MRGTPRDLLP GDPICSPVAA VLAEATIQLI 2220
RILHRTDRWT YCINKKMMER LHKIKICIKE SGQKLKKSRS VQSREENEMR EEKESKEEEK 2280
GKHTRHGLAD LSELQLRTLC IEVWPVLAVI GGVDAGLRVG GRCVHKQTGR HATLLGVVKE 2340
GSTSAKVQWD EAEITISFPT FWSPSDTPLY NLEPCEPLPF DVARFRGLTA SVLLDLTYLT 2400
GVHEDMGKQS TKRHEKKHRH ESEEKGDVEQ KPESESALDM RTGLTSDDVK SQSTTSSKSE 2460
NEIASFSLDP TLPSVESQHQ ITEGKRKNHE HMSKNHDVAQ SEIRAVQLSY LYLGAMKSLS 2520
ALLGCSKYAE LLLIPKVLAE NGHNSDCASS PVVHEDVEMR AALQFLMRHM VKRAVMRSPI 2580
KRALGLADLE RAQAMIYKLV VHGLLEDQFG GKIKQEIDQQ AEESDPAQQA QTPVTTSPSA 2640
SSTTSFMSSS LEDTTTATTP VTDTETVPAS ESPGVMPLSL LRQMFSSYPT TTVLPTRRAQ 2700
TPPISSLPTS PSDEVGRRQS LTSPDSQSAR PANRTALSDP SSRLSTSPPP PAIAVPLLEM 2760
GFSLRQIAKA MEATGARGEA DAQNITVLAM WMIEHPGHED EEEPQSGSTA DSRPGAAVLG 2820
SGGKSNDPCY LQSPGDIPSA DAAEMEEGFS ESPDNLDHTE NAASGSGPSA RGRSAVTRRH 2880
KFDLAARTLL ARAAGLYRSV QAHRNQSRRE GISLQQDPGA LYDFNLDEEL EIDLDDEAME 2940
AMFGQDLTSD NDILGMWIPE VLDWPTWHVC ESEDREEVVV CELCECSVVS FNQHMKRNHP 3000
GCGRSANRQG YRSNGSYVDG WFGGECGSGN PYYLLCGTCR EKYLAMKTKS KSTSSERYKG 3060
QAPDLIGKQD SVYEEDWDML DVDEDEKLTG EEEFELLAGP LGLNDRRIVP EPVQFPDSDP 3120
LGASVAMVTA TNSMEETLMQ IGCHGSVEKS SSGRITLGEQ AAALANPHDR VVALRRVTAA 3180
AQVLLARTMV MRALSLLSVS GSSCSLAAGL ESLGLTDIRT LVRLMCLAAA GRAGLSTSPS 3240
AMASTSERSR GGHSKANKPI SCLAYLSTAV GCLASNAPSA AKLLVQLCTQ NLISAATGVN 3300
LTTVDDSIQR KFLPSFLRGI AEENKLVTSP NFVVTQALVA LLADKGAKLR PNYDKSEVEK 3360
KGPLELANAL AACCLSSRLS SQHRQWAAQQ LVRTLAAHDR DNQTTLQTLA DMGGDLRKCS 3420
FIKLEAHQNR VMTCVWCNKK GLLATSGNDG TIRVWNVTKK QYSLQQTCVF NRLEGDAEES 3480
LGSPSDPSFS PVSWSISGKY LAGALEKMVN IWQVNGGKGL VDIQPHWVSA LAWPEEGPAT 3540
AWSGESPELL LVGRMDGSLG LIEVVDVSTM HRRELEHCYR KDVSVTCIAW FSEDRPFAVG 3600
YFDGKLLLGT KEPLEKGGIV LIDAHKDTLI SMKWDPTGHI LMTCAKEDSV KLWGSISGCW 3660
CCLHSLCHPS IVNGIAWCRL PGKGSKLQLL MATGCQSGLV CVWRIPQDTT QTNVTSAEGW 3720
WEQESNCQDG YRKSSGAKCV YQLRGHITPV RTVAFSSDGL ALVSGGLGGL MNIWSLRDGS 3780
VLQTVVIGSG AIQTTVWIPE VGVAACSNRS KDVLVVNCTA EWAAANHVLA TCRTALKQQG 3840
VLGLNMAPCM RAFLERLPMM LQEQYAYEKP HVVCGDQLVH SPYMQCLASL AVGLHLDQLL 3900
CNPPVPPHHQ NCLPDPASWN PNEWAWLECF STTIKAAEAL TNGAQFPESF TVPDLEPVPE 3960
DELVFLMDNS KWINGMDEQI MSWATSRPED WHLGGKCDVY LWGAGRHGQL AEAGRNVMVP 4020
AAAPSFSQAQ QVICGQNCTF VIQANGTVLA CGEGSYGRLG QGNSDDLHVL TVISALQGFV 4080
VTQLVTSCGS DGHSMALTES GEVFSWGDGD YGKLGHGNSD RQRRPRQIEA LQGEEVVQMS 4140
CGFKHSAVVT SDGKLFTFGN GDYGRLGLGN TSNKKLPERV TALEGYQIGQ VACGLNHTLA 4200
VSADGSMVWA FGDGDYGKLG LGNSTAKSSP QKIDVLCGIG IKKVACGTQF SVALTKDGHV 4260
YTFGQDRLIG LPEGRARNHN RPQQIPVLAG VIIEDVAVGA EHTLALASNG DVYAWGSNSE 4320
GQLGLGHTNH VREPTLVTGL QGKNVRQISA GRCHSAAWTA PPVPPRAPGV SVPLQLGLPD 4380
TVPPQYGALR EVSIHTVRAR LRLLYHFSDL MYSSWRLLNL SPNNQNSTSH YNAGTWGIVQ 4440
GQLRPLLAPR VYTLPMVRSI GKTMVQGKNY GPQITVKRIS TRGRKCKPIF VQIARQVVKL 4500
NASDLRLPSR AWKVKLVGEG ADDAGGVFDD TITEMCQELE TGIVDLLIPS PNATAEVGYN 4560
RDRFLFNPSA CLDEHLMQFK FLGILMGVAI RTKKPLDLHL APLVWKQLCC VPLTLEDLEE 4620
VDLLYVQTLN SILHIEDSGI TEESFHEMIP LDSFVGQSAD GKMVPIIPGG NSIPLTFSNR 4680
KEYVERAIEY RLHEMDRQVA AVREGMSWIV PVPLLSLLTA KQLEQMVCGM PEISVEVLKK 4740
VVRYREVDEQ HQLVQWFWHT LEEFSNEERV LFMRFVSGRS RLPANTADIS QRFQIMKVDR 4800
PYDSLPTSQT CFFQLRLPPY SSQLVMAERL RYAINNCRSI DMDNYMLSRN VDNAEGSDTD 4860
Y 4861 
Gene Ontology
 GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; TAS:ProtInc.
 GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
 GO:0005086; F:ARF guanyl-nucleotide exchange factor activity; TAS:ProtInc.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:InterPro.
 GO:0006810; P:transport; IEA:UniProtKB-KW. 
Interpro
 IPR001870; B30.2/SPRY.
 IPR008985; ConA-like_lec_gl_sf.
 IPR000569; HECT.
 IPR009091; RCC1/BLIP-II.
 IPR000408; Reg_chr_condens.
 IPR018355; SPla/RYanodine_receptor_subgr.
 IPR003877; SPRY_rcpt.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00632; HECT
 PF00415; RCC1
 PF00622; SPRY
 PF00400; WD40 
SMART
 SM00119; HECTc
 SM00449; SPRY
 SM00320; WD40 
PROSITE
 PS50188; B302_SPRY
 PS50237; HECT
 PS00625; RCC1_1
 PS00626; RCC1_2
 PS50012; RCC1_3
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00633; RCCNDNSATION.