UniProt Accession

 PCH2_HUMAN; Q15645; C9K0T3; D3DTC0; O15324 

Genbank Protein ID

 U96131; CR456744; AC122719; CH471102; CH471102; BC000404; BC019294; L40384 

Genbank Nucleotide ID

 AAB64095.1; CAG33025.1; EAX08185.1; EAX08186.1; AAH00404.1; AAH19294.1; AAC41732.1 

Protein Name

 Pachytene checkpoint protein 2 homolog 

Protein Synonyms/Alias

 Human papillomavirus type 16 E1 protein-binding protein; 16E1-BP; HPV16 E1 protein-binding protein; Thyroid hormone receptor interactor 13; Thyroid receptor-interacting protein 13; TR-interacting protein 13; TRIP-13 

Gene Name

 TRIP13 

Gene Synonyms/Alias

 PCH2 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
34	QRGSSTAKKEDINLS	ubiquitination	[1]
35	RGSSTAKKEDINLSV	ubiquitination	[1]
185	HGPPGTGKTSLCKAL	ubiquitination	[1, 2, 3]
195	LCKALAQKLTIRLSS	ubiquitination	[1]
227	KWFSESGKLVTKMFQ	ubiquitination	[1, 2, 3, 4]
288	LTQIDQIKRHSNVVI	ubiquitination	[1, 2, 3, 4, 5, 6]
316	FVDRADIKQYIGPPS	ubiquitination	[1, 2, 3, 4, 5, 6]
340	SCLEELMKCQIIYPR	ubiquitination	[5]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572] 

Functional Description

 Plays a key role in chromosome recombination and chromosome structure development during meiosis. Required at early steps in meiotic recombination that leads to non-crossovers pathways. Also needed for efficient completion of homologous synapsis by influencing crossover distribution along the chromosomes affecting both crossovers and non-crossovers pathways. Also required for development of higher-order chromosome structures and is needed for synaptonemal-complex formation. In males, required for efficient synapsis of the sex chromosomes and for sex body formation. Promotes early steps of the DNA double- strand breaks (DSBs) repair process upstream of the assembly of RAD51 complexes. Required for depletion of HORMAD1 and HORMAD2 from synapsed chromosomes (By similarity). 

Sequence Annotation

 NP_BIND 179 186 ATP (Potential).
 MOD_RES 1 1 N-acetylmethionine.  

Keyword

 Acetylation; Alternative splicing; ATP-binding; Complete proteome; Differentiation; Meiosis; Nucleotide-binding; Oogenesis; Reference proteome; Spermatogenesis. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 432 AA 

Protein Sequence

Gene Ontology

 GO:0001673; C:male germ cell nucleus; IEA:Compara.
 GO:0005634; C:nucleus; TAS:ProtInc.
 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0017111; F:nucleoside-triphosphatase activity; IEA:InterPro.
 GO:0003712; F:transcription cofactor activity; TAS:ProtInc.
 GO:0006302; P:double-strand break repair; ISS:UniProtKB.
 GO:0007144; P:female meiosis I; IEA:Compara.
 GO:0007141; P:male meiosis I; IEA:Compara.
 GO:0001556; P:oocyte maturation; IEA:Compara.
 GO:0048477; P:oogenesis; ISS:UniProtKB.
 GO:0007131; P:reciprocal meiotic recombination; ISS:UniProtKB.
 GO:0007286; P:spermatid development; IEA:Compara.
 GO:0007283; P:spermatogenesis; ISS:UniProtKB.
 GO:0007130; P:synaptonemal complex assembly; ISS:UniProtKB.
 GO:0006366; P:transcription from RNA polymerase II promoter; TAS:ProtInc. 

Interpro

 IPR003593; AAA+_ATPase.
 IPR003959; ATPase_AAA_core.
 IPR003960; ATPase_AAA_CS.
 IPR001270; Chaprnin_ClpA/B.
 IPR027417; P-loop_NTPase. 

Pfam

 PF00004; AAA 

SMART

 SM00382; AAA 

PROSITE

 PS00674; AAA 

PRINTS

 PR00300; CLPPROTEASEA.