UniProt Accession

 DPH5_MOUSE; Q9CWQ0; Q6PAC5 

Genbank Protein ID

 AK010475; AC131113; CH466532; BC060372 

Genbank Nucleotide ID

 BAB26968.1; EDL12395.1; AAH60372.1 

Protein Name

 Diphthine synthase 

Protein Synonyms/Alias

 Diphthamide biosynthesis methyltransferase 

Gene Name

 Dph5 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
18	DAKDITVKGLEVVRR	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961] 

Functional Description

 S-adenosyl-L-methionine-dependent methyltransferase that catalyzes the trimethylation of the amino group of the modified target histidine residue in translation elongation factor 2 (EF- 2), to form an intermediate called diphthine. The three successive methylation reactions represent the second step of diphthamide biosynthesis. 

Sequence Annotation

 REGION 112 113 S-adenosyl-L-methionine binding (By
 BINDING 9 9 S-adenosyl-L-methionine; via carbonyl
 BINDING 84 84 S-adenosyl-L-methionine; via amide
 BINDING 87 87 S-adenosyl-L-methionine; via carbonyl
 BINDING 163 163 S-adenosyl-L-methionine; via amide
 BINDING 225 225 S-adenosyl-L-methionine; via amide
 BINDING 250 250 S-adenosyl-L-methionine; via carbonyl  

Keyword

 Complete proteome; Methyltransferase; Reference proteome; S-adenosyl-L-methionine; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 281 AA 

Protein Sequence

Gene Ontology

 GO:0004164; F:diphthine synthase activity; IEA:EC.
 GO:0017183; P:peptidyl-diphthamide biosynthetic process from peptidyl-histidine; IEA:UniProtKB-UniPathway. 

Interpro

 IPR000878; 4pyrrol_Mease.
 IPR014777; 4pyrrole_Mease_sub1.
 IPR014776; 4pyrrole_Mease_sub2.
 IPR004551; Dphthn_synthase. 

Pfam

 PF00590; TP_methylase 

SMART

  

PROSITE

  

PRINTS