CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002592
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Gap junction alpha-1 protein 
Protein Synonyms/Alias
 Connexin-43; Cx43; Gap junction 43 kDa heart protein 
Gene Name
 Gja1 
Gene Synonyms/Alias
 Cxn-43 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
109KEEKLNKKEEELKVAacetylation[1]
109KEEKLNKKEEELKVAubiquitination[2]
128VNVEMHLKQIEIKKFubiquitination[2]
133HLKQIEIKKFKYGIEacetylation[1]
136QIEIKKFKYGIEEHGubiquitination[2]
144YGIEEHGKVKMRGGLacetylation[1]
303SSCRNYNKQASEQNWubiquitination[2]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). 
Sequence Annotation
 MOD_RES 247 247 Phosphotyrosine (By similarity).
 MOD_RES 255 255 Phosphoserine (By similarity).
 MOD_RES 262 262 Phosphoserine (By similarity).
 MOD_RES 271 271 S-nitrosocysteine (By similarity).
 MOD_RES 306 306 Phosphoserine (By similarity).
 MOD_RES 313 313 Phosphotyrosine (By similarity).
 MOD_RES 314 314 Phosphoserine (By similarity).
 MOD_RES 325 325 Phosphoserine; by CK1 (By similarity).
 MOD_RES 326 326 Phosphothreonine (By similarity).
 MOD_RES 328 328 Phosphoserine; by CK1 (By similarity).
 MOD_RES 330 330 Phosphoserine; by CK1 (By similarity).
 MOD_RES 365 365 Phosphoserine.
 MOD_RES 368 368 Phosphoserine; by PKC/PRKCG (Probable).
 MOD_RES 369 369 Phosphoserine.
 MOD_RES 373 373 Phosphoserine.
 DISULFID 54 192 By similarity.
 DISULFID 187 198 By similarity.
 CROSSLNK 144 144 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 237 237 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Cell junction; Cell membrane; Complete proteome; Direct protein sequencing; Disulfide bond; Gap junction; Isopeptide bond; Membrane; Phosphoprotein; Reference proteome; S-nitrosylation; Transmembrane; Transmembrane helix; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 382 AA 
Protein Sequence
MGDWSALGKL LDKVQAYSTA GGKVWLSVLF IFRILLLGTA VESAWGDEQS AFRCNTQQPG 60
CENVCYDKSF PISHVRFWVL QIIFVSVPTL LYLAHVFYVM RKEEKLNKKE EELKVAQTDG 120
VNVEMHLKQI EIKKFKYGIE EHGKVKMRGG LLRTYIISIL FKSVFEVAFL LIQWYIYGFS 180
LSAVYTCKRD PCPHQVDCFL SRPTEKTIFI IFMLVVSLVS LALNIIELFY VFFKGVKDRV 240
KGRSDPYHAT TGPLSPSKDC GSPKYAYFNG CSSPTAPLSP MSPPGYKLVT GDRNNSSCRN 300
YNKQASEQNW ANYSAEQNRM GQAGSTISNS HAQPFDFPDD NQNAKKVAAG HELQPLAIVD 360
QRPSSRASSR ASSRPRPDDL EI 382 
Gene Ontology
 GO:0016324; C:apical plasma membrane; IEA:Compara.
 GO:0005922; C:connexon complex; IDA:RGD.
 GO:0043292; C:contractile fiber; IEA:Compara.
 GO:0005829; C:cytosol; IEA:Compara.
 GO:0005769; C:early endosome; IDA:RGD.
 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0005916; C:fascia adherens; IDA:RGD.
 GO:0030660; C:Golgi-associated vesicle membrane; TAS:Reactome.
 GO:0005887; C:integral to plasma membrane; TAS:UniProtKB.
 GO:0005882; C:intermediate filament; IEA:Compara.
 GO:0016328; C:lateral plasma membrane; IEA:Compara.
 GO:0005764; C:lysosome; IDA:RGD.
 GO:0045121; C:membrane raft; IDA:BHF-UCL.
 GO:0005741; C:mitochondrial outer membrane; IDA:RGD.
 GO:0005771; C:multivesicular body; IDA:RGD.
 GO:0005243; F:gap junction channel activity; IDA:RGD.
 GO:0019904; F:protein domain specific binding; IDA:RGD.
 GO:0004871; F:signal transducer activity; IEA:Compara.
 GO:0007512; P:adult heart development; IEA:Compara.
 GO:0006915; P:apoptotic process; IMP:RGD.
 GO:0015867; P:ATP transport; IMP:RGD.
 GO:0003294; P:atrial ventricular junction remodeling; IEA:Compara.
 GO:0048514; P:blood vessel morphogenesis; IEA:Compara.
 GO:0061337; P:cardiac conduction; IEA:Compara.
 GO:0010643; P:cell communication by chemical coupling; IEA:Compara.
 GO:0010644; P:cell communication by electrical coupling; IEA:Compara.
 GO:0007267; P:cell-cell signaling; IDA:RGD.
 GO:0071260; P:cellular response to mechanical stimulus; IEP:RGD.
 GO:0002544; P:chronic inflammatory response; IMP:RGD.
 GO:0007204; P:elevation of cytosolic calcium ion concentration; IMP:RGD.
 GO:0003158; P:endothelium development; IEP:RGD.
 GO:0002070; P:epithelial cell maturation; IEA:Compara.
 GO:0016264; P:gap junction assembly; TAS:UniProtKB.
 GO:0007507; P:heart development; IEP:RGD.
 GO:0001947; P:heart looping; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0002088; P:lens development in camera-type eye; IEA:Compara.
 GO:0060174; P:limb bud formation; IEA:Compara.
 GO:0060156; P:milk ejection; IEA:Compara.
 GO:0060044; P:negative regulation of cardiac muscle cell proliferation; IMP:RGD.
 GO:0010629; P:negative regulation of gene expression; IEA:Compara.
 GO:0001764; P:neuron migration; IEA:Compara.
 GO:0048812; P:neuron projection morphogenesis; IMP:RGD.
 GO:0001649; P:osteoblast differentiation; IEA:Compara.
 GO:2000987; P:positive regulation of behavioral fear response; IMP:RGD.
 GO:0010652; P:positive regulation of cell communication by chemical coupling; IMP:RGD.
 GO:0010628; P:positive regulation of gene expression; IEA:Compara.
 GO:0003104; P:positive regulation of glomerular filtration; IMP:RGD.
 GO:0043123; P:positive regulation of I-kappaB kinase/NF-kappaB cascade; IEA:Compara.
 GO:0045732; P:positive regulation of protein catabolic process; IDA:RGD.
 GO:0045844; P:positive regulation of striated muscle tissue development; IEA:Compara.
 GO:0045907; P:positive regulation of vasoconstriction; IMP:RGD.
 GO:0045909; P:positive regulation of vasodilation; IMP:RGD.
 GO:0051259; P:protein oligomerization; IDA:RGD.
 GO:0060371; P:regulation of atrial cardiac muscle cell membrane depolarization; IEA:Compara.
 GO:0030500; P:regulation of bone mineralization; IEA:Compara.
 GO:0046850; P:regulation of bone remodeling; IEA:Compara.
 GO:0051924; P:regulation of calcium ion transport; IMP:RGD.
 GO:2000810; P:regulation of tight junction assembly; IMP:RGD.
 GO:0060373; P:regulation of ventricular cardiac muscle cell membrane depolarization; IEA:Compara.
 GO:0060307; P:regulation of ventricular cardiac muscle cell membrane repolarization; IEA:Compara.
 GO:0034405; P:response to fluid shear stress; IEP:RGD.
 GO:0043434; P:response to peptide hormone stimulus; IEP:RGD.
 GO:0009268; P:response to pH; IDA:RGD.
 GO:0043403; P:skeletal muscle tissue regeneration; IEA:Compara.
 GO:0010232; P:vascular transport; IMP:RGD. 
Interpro
 IPR000500; Connexin.
 IPR002261; Connexin43.
 IPR013124; Connexin43_C.
 IPR019570; Connexin_CCC.
 IPR017990; Connexin_CS.
 IPR013092; Connexin_N. 
Pfam
 PF00029; Connexin
 PF03508; Connexin43
 PF10582; Connexin_CCC 
SMART
 SM00037; CNX
 SM01089; Connexin_CCC 
PROSITE
 PS00407; CONNEXINS_1
 PS00408; CONNEXINS_2 
PRINTS
 PR00206; CONNEXIN.
 PR01132; CONNEXINA1.