UniProt Accession

 NDKB_HUMAN; P22392; A8MWA3; Q1WM23; Q6LCT6 

Genbank Protein ID

 X58965; M36981; L16785; DQ109675; AC005839; BC002476; BC133029; BC133031; U29200 

Genbank Nucleotide ID

 CAB37870.1; AAA36369.1; AAA60228.1; AAZ82097.1; AAH02476.1; AAI33030.1; AAI33032.1; AAA86745.1 

Protein Name

 Nucleoside diphosphate kinase B 

Protein Synonyms/Alias

 NDK B; NDP kinase B; C-myc purine-binding transcription factor PUF; Histidine protein kinase NDKB; nm23-H2 

Gene Name

 NME2 

Gene Synonyms/Alias

 NM23B 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
12	ERTFIAIKPDGVQRG	ubiquitination	[1]
26	GLVGEIIKRFEQKGF	ubiquitination	[1]
39	GFRLVGLKFMQASED	ubiquitination	[1]
49	QASEDLLKEHYVDLK	ubiquitination	[1]
56	KEHYVDLKDRPFFAG	ubiquitination	[1]
85	WEGLNVVKTGRVMLG	ubiquitination	[1]
100	ETNPADSKPGTIRGD	ubiquitination	[1]
164	RASEEHLKQHYIDLK	ubiquitination	[1]
171	KQHYIDLKDRPFFPG	ubiquitination	[1]
200	WEGLNVVKTGRVMLG	ubiquitination	[1]
239	IHGSDSVKSAEKEIS	ubiquitination	[1]
243	DSVKSAEKEISLWFK	ubiquitination	[1]
250	KEISLWFKPEELVDY	ubiquitination	[1]

Reference

 [1] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661] 

Functional Description

 Major role in the synthesis of nucleoside triphosphates other than ATP. Negatively regulates Rho activity by interacting with AKAP13/LBC. Acts as a transcriptional activator of the MYC gene; binds DNA non-specifically (PubMed:8392752). Exhibits histidine protein kinase activity. 

Sequence Annotation

 REGION 2 66 Interaction with AKAP13.
 ACT_SITE 118 118 Pros-phosphohistidine intermediate.
 BINDING 12 12 ATP.
 BINDING 60 60 ATP.
 BINDING 88 88 ATP.
 BINDING 94 94 ATP.
 BINDING 105 105 ATP.
 BINDING 115 115 ATP.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 94 94 Phosphothreonine (By similarity).
 MOD_RES 128 128 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; Activator; Alternative splicing; ATP-binding; Cell projection; Complete proteome; Cytoplasm; Direct protein sequencing; DNA-binding; Kinase; Magnesium; Metal-binding; Nucleotide metabolism; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Transcription; Transcription regulation; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 152 AA 

Protein Sequence

Gene Ontology

 GO:0071944; C:cell periphery; IDA:UniProtKB.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0030027; C:lamellipodium; IDA:HGNC.
 GO:0005634; C:nucleus; NAS:UniProtKB.
 GO:0001726; C:ruffle; IDA:HGNC.
 GO:0005524; F:ATP binding; NAS:UniProtKB.
 GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
 GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
 GO:0004550; F:nucleoside diphosphate kinase activity; IDA:UniProtKB.
 GO:0004673; F:protein histidine kinase activity; IEA:EC.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; TAS:UniProtKB.
 GO:0007155; P:cell adhesion; TAS:HGNC.
 GO:0006241; P:CTP biosynthetic process; IEA:InterPro.
 GO:0006183; P:GTP biosynthetic process; IEA:InterPro.
 GO:0007229; P:integrin-mediated signaling pathway; IDA:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; IMP:HGNC.
 GO:0015949; P:nucleobase-containing small molecule interconversion; TAS:Reactome.
 GO:0009142; P:nucleoside triphosphate biosynthetic process; IDA:UniProtKB.
 GO:0050679; P:positive regulation of epithelial cell proliferation; IMP:HGNC.
 GO:0045618; P:positive regulation of keratinocyte differentiation; IMP:HGNC.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006228; P:UTP biosynthetic process; IEA:InterPro. 

Interpro

 IPR001564; Nucleoside_diP_kinase.
 IPR023005; Nucleoside_diP_kinase_AS. 

Pfam

 PF00334; NDK 

SMART

 SM00562; NDK 

PROSITE

 PS00469; NDP_KINASES 

PRINTS

 PR01243; NUCDPKINASE.