CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006219
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Actin cytoskeleton-regulatory complex protein SLA1 
Protein Synonyms/Alias
  
Gene Name
 SLA1 
Gene Synonyms/Alias
 YBL007C; YBL0321 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
181RARMMQSKEDQAPDEubiquitination[1]
284KINFIPQKGTPHEWSubiquitination[1]
302LVSYDNEKKHMFLEFubiquitination[1]
303VSYDNEKKHMFLEFVacetylation[2]
353REVEMASKSKKRGIVubiquitination[1]
386KVYILDDKKSKDWWMubiquitination[1]
389ILDDKKSKDWWMCQLubiquitination[1]
428STASGIIKSIKKNFTubiquitination[1]
436SIKKNFTKSPSRSRSubiquitination[1]
456SNANASWKDDELQNDubiquitination[1]
471VVGSAAGKRSRKSSLubiquitination[1, 3]
489KKNSSATKDFPNPKKubiquitination[1]
508VDRSGTFKVDAEFIGubiquitination[1]
518AEFIGCAKGKIHLHKubiquitination[3]
537KIAVAADKLSNEDLAubiquitination[1]
548EDLAYVEKITGFSLEubiquitination[3]
556ITGFSLEKFKANDGSacetylation[2]
556ITGFSLEKFKANDGSubiquitination[1, 3]
558GFSLEKFKANDGSSSubiquitination[1]
602ERELYELKKARELLDubiquitination[1]
624EKELPPIKPPRPTSTubiquitination[1]
792SESNLLSKKSEFTGSubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Component of the PAN1 actin cytoskeleton-regulatory complex required for the internalization of endosomes during actin-coupled endocytosis. The complex links the site of endocytosis to the cell membrane-associated actin cytoskeleton. Mediates uptake of external molecules and vacuolar degradation of plasma membrane proteins. Plays a role in the proper organization of the cell membrane-associated actin cytoskeleton and promotes its destabilization. 
Sequence Annotation
 DOMAIN 8 69 SH3 1.
 DOMAIN 70 132 SH3 2.
 DOMAIN 353 415 SH3 3.
 REPEAT 868 874 1.
 REPEAT 877 883 2.
 REPEAT 887 893 3.
 REPEAT 923 929 4.
 REPEAT 945 951 5.
 REPEAT 1003 1009 6.
 REPEAT 1020 1026 7.
 REPEAT 1031 1037 8.
 REPEAT 1048 1054 9.
 REPEAT 1065 1071 10.
 REPEAT 1084 1090 11.
 REPEAT 1129 1135 12.
 REPEAT 1155 1161 13.
 REPEAT 1170 1176 14.
 REPEAT 1185 1191 15.
 REPEAT 1200 1206 16.
 REGION 868 1205 16 X 7 AA approximate repeats of T-G-G-A-
 MOD_RES 447 447 Phosphoserine.
 MOD_RES 449 449 Phosphoserine.
 MOD_RES 454 454 Phosphoserine.
 MOD_RES 799 799 Phosphoserine.
 MOD_RES 831 831 Phosphothreonine.
 MOD_RES 858 858 Phosphothreonine.
 MOD_RES 887 887 Phosphothreonine.
 MOD_RES 904 904 Phosphothreonine.
 MOD_RES 984 984 Phosphothreonine.
 MOD_RES 993 993 Phosphothreonine.
 MOD_RES 996 996 Phosphoserine.
 MOD_RES 1075 1075 Phosphothreonine.  
Keyword
 3D-structure; Actin-binding; Cell membrane; Complete proteome; Cytoplasm; Cytoskeleton; Endocytosis; Endosome; Membrane; Nucleus; Phosphoprotein; Reference proteome; Repeat; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1244 AA 
Protein Sequence
MTVFLGIYRA VYAYEPQTPE ELAIQEDDLL YLLQKSDIDD WWTVKKRVIG SDSEEPVGLV 60
PSTYIEEAPV LKKVRAIYDY EQVQNADEEL TFHENDVFDV FDDKDADWLL VKSTVSNEFG 120
FIPGNYVEPE NGSTSKQEQA PAAAEAPAAT PAAAPASAAV LPTNFLPPPQ HNDRARMMQS 180
KEDQAPDEDE EGPPPAMPAR PTATTETTDA TAAAVRSRTR LSYSDNDNDD EEDDYYYNSN 240
SNNVGNHEYN TEYHSWNVTE IEGRKKKKAK LSIGNNKINF IPQKGTPHEW SIDKLVSYDN 300
EKKHMFLEFV DPYRSLELHT GNTTTCEEIM NIIGEYKGAS RDPGLREVEM ASKSKKRGIV 360
QYDFMAESQD ELTIKSGDKV YILDDKKSKD WWMCQLVDSG KSGLVPAQFI EPVRDKKHTE 420
STASGIIKSI KKNFTKSPSR SRSRSRSKSN ANASWKDDEL QNDVVGSAAG KRSRKSSLSS 480
HKKNSSATKD FPNPKKSRLW VDRSGTFKVD AEFIGCAKGK IHLHKANGVK IAVAADKLSN 540
EDLAYVEKIT GFSLEKFKAN DGSSSRGTDS RDSERERRRR LKEQEEKERD RRLKERELYE 600
LKKARELLDE ERSRLQEKEL PPIKPPRPTS TTSVPNTTSV PPAESSNNNN SSNKYDWFEF 660
FLNCGVDVSN CQRYTINFDR EQLTEDMMPD INNSMLRTLG LREGDIVRVM KHLDKKFGRE 720
NIASIPTNAT GNMFSQPDGS LNVATSPETS LPQQLLPQTT SPAQTAPSTS AETDDAWTVK 780
PASKSESNLL SKKSEFTGSM QDLLDLQPLE PKKAAASTPE PNLKDLEPVK TGGTTVPAAP 840
VSSAPVSSAP APLDPFKTGG NNILPLSTGF VMMPMITGGD MLPMQRTGGF VVPQTTFGMQ 900
SQVTGGILPV QKTGNGLIPI SNTGGAMMPQ TTFGAAATVL PLQKTGGGLI PIATTGGAQF 960
PQTSFNVQGQ QQLPTGSILP VQKTANGLIS ANTGVSMPTV QRTGGTMIPQ TSFGVSQQLT 1020
GGAMMTQPQN TGSAMMPQTS FNAVPQITGG AMMPQTSFNA LPQVTGGAMM PLQRTGGALN 1080
TFNTGGAMIP QTSFSSQAQN TGGFRPQSQF GLTLQKTGGI APLNQNQFTG GAMNTLSTGG 1140
VLQQQQPQTM NTFNTGGVMQ ELQMMTTFNT GGAMQQPQMM NTFNTDGIMQ QPQMMNTFNT 1200
GGAMQQPQQQ ALQNQPTGFG FGNGPQQSRQ ANIFNATASN PFGF 1244 
Gene Ontology
 GO:0030479; C:actin cortical patch; IDA:SGD.
 GO:0010008; C:endosome membrane; IEA:UniProtKB-SubCell.
 GO:0043332; C:mating projection tip; IDA:SGD.
 GO:0005634; C:nucleus; IMP:SGD.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0043130; F:ubiquitin binding; IDA:SGD.
 GO:0000147; P:actin cortical patch assembly; TAS:SGD.
 GO:0006897; P:endocytosis; IPI:SGD.
 GO:0031505; P:fungal-type cell wall organization; TAS:SGD. 
Interpro
 IPR001452; SH3_domain.
 IPR007131; SHD1. 
Pfam
 PF00018; SH3_1
 PF03983; SHD1 
SMART
 SM00326; SH3 
PROSITE
 PS50002; SH3 
PRINTS
 PR00452; SH3DOMAIN.