UniProt Accession

 SPCS3_HUMAN; P61009; P12280; Q9H0S7 

Genbank Protein ID

 AL136660; AK026302; AY359044; BC047290 

Genbank Nucleotide ID

 CAB66595.1; BAB15437.1; AAQ89403.1; AAH47290.1 

Protein Name

 Signal peptidase complex subunit 3 

Protein Synonyms/Alias

 Microsomal signal peptidase 22/23 kDa subunit; SPC22/23; SPase 22/23 kDa subunit 

Gene Name

 SPCS3 

Gene Synonyms/Alias

 SPC22; UNQ1841/PRO3567 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
49	HVSRIMLKNVEDFTG	ubiquitination	[1, 2, 3, 4, 5, 6, 7, 8, 9]
108	NQVVLWDKIVLRGDN	ubiquitination	[2, 6]
117	VLRGDNPKLLLKDMK	ubiquitination	[1, 2, 6, 8]
121	DNPKLLLKDMKTKYF	ubiquitination	[2, 6]
126	LLKDMKTKYFFFDDG	ubiquitination	[2, 6, 7]
137	FDDGNGLKGNRNVTL	ubiquitination	[1, 2, 3, 4, 6, 7, 8, 9]

Reference

 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
 Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
 J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
 [5] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [6] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [7] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [8] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 

Functional Description

 Component of the microsomal signal peptidase complex which removes signal peptides from nascent proteins as they are translocated into the lumen of the endoplasmic reticulum (By similarity). 

Sequence Annotation

 CARBOHYD 141 141 N-linked (GlcNAc...).  

Keyword

 Complete proteome; Endoplasmic reticulum; Glycoprotein; Hydrolase; Membrane; Microsome; Protease; Reference proteome; Signal-anchor; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 180 AA 

Protein Sequence

Gene Ontology

 GO:0005789; C:endoplasmic reticulum membrane; TAS:Reactome.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005787; C:signal peptidase complex; IEA:InterPro.
 GO:0008233; F:peptidase activity; IEA:UniProtKB-KW.
 GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
 GO:0050796; P:regulation of insulin secretion; TAS:Reactome.
 GO:0006465; P:signal peptide processing; IEA:InterPro.
 GO:0006614; P:SRP-dependent cotranslational protein targeting to membrane; TAS:Reactome.
 GO:0006412; P:translation; TAS:Reactome. 

Interpro

 IPR007653; SPC22. 

Pfam

 PF04573; SPC22 

SMART

  

PROSITE

  

PRINTS