CPLM-020127

Genbank Nucleotide ID

Ubiquitin-associated domain-containing protein 1

Protein Synonyms/Alias

UBA domain-containing protein 1; E3 ubiquitin-protein ligase subunit KPC2; Glialblastoma cell differentiation-related protein 1; Kip1 ubiquitination-promoting complex protein 2

Homo sapiens (Human)

Position	Peptide	Type	References
7	*MFVQEEKIFAGKVL	ubiquitination	[1, 2]
12	EEKIFAGKVLRLHIC	ubiquitination	[3]
56	HGSLEDPKSITHHKL	ubiquitination	[4]
111	ADVSAEEKKKQDQKA	ubiquitination	[5]
112	DVSAEEKKKQDQKAP	ubiquitination	[3]
121	QDQKAPDKEAILRAT	ubiquitination	[1, 2, 3, 4]
154	DFQTELRKILVSLIE	ubiquitination	[1, 2]
178	PDAVELFKKANAMLD	ubiquitination	[1, 2, 5, 6, 7, 8]
179	DAVELFKKANAMLDE	ubiquitination	[3, 4, 5]
305	MEMGFDEKEVIDALR	ubiquitination	[8, 9]
330	EWLLGDRKPSPEELD	ubiquitination	[4, 5, 8]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[5] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[6] Global analysis of lysine ubiquitination by ubiquitin remnant immunoaffinity profiling.
Xu G, Paige JS, Jaffrey SR.
Nat Biotechnol. 2010 Aug;28(8):868-73. [PMID: 20639865]
[7] Global identification of modular cullin-RING ligase substrates.
Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
[8] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[9] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

Non-catalytic subunit of the KPC complex that acts as E3 ubiquitin-protein ligase. Required for poly-ubiquitination and proteasome-mediated degradation of CDKN1B during G1 phase of the cell cycle.

DOMAIN 14 98 Ubiquitin-like.
DOMAIN 187 231 UBA 1.
DOMAIN 288 328 UBA 2.
DOMAIN 353 392 STI1.

3D-structure; Complete proteome; Cytoplasm; Polymorphism; Reference proteome; Repeat; Ubl conjugation pathway.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005794; C:Golgi apparatus; IDA:HPA.
GO:0005886; C:plasma membrane; IDA:HPA.
GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.

IPR006636; STI1_HS-bd.
IPR009060; UBA-like.
IPR000449; UBA/transl_elong_EF1B_N.
IPR015940; UBA/transl_elong_EF1B_N_euk.

SM00727; STI1
SM00165; UBA

PS50030; UBA
PS50053; UBIQUITIN_2