CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023988
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitotic spindle assembly checkpoint protein MAD1 
Protein Synonyms/Alias
 Mitotic arrest deficient 1-like protein 1; MAD1-like protein 1; Mitotic checkpoint MAD1 protein homolog; HsMAD1; hMAD1; Tax-binding protein 181 
Gene Name
 MAD1L1 
Gene Synonyms/Alias
 MAD1; TXBP181 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
61RAEQIRSKSHLIQVEacetylation[1]
61RAEQIRSKSHLIQVEubiquitination[2]
79MQMELSHKRARVELEubiquitination[2]
143QNLDAASKRLREKEDubiquitination[2]
313ENERLLAKLQSWERLubiquitination[2]
406KRVLLLTKERDGMRAubiquitination[3]
481EMELKMLKSQSSSAEubiquitination[2, 3]
504EADTLRLKVEELEGEubiquitination[2]
614QVESAELKNQRLKEVubiquitination[2]
625LKEVFQTKIQEFRKAubiquitination[4]
Reference
 [1] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 
Functional Description
 Component of the spindle-assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. May recruit MAD2L1 to unattached kinetochores. Has a role in the correct positioning of the septum. Required for anchoring MAD2L1 to the nuclear periphery. Binds to the TERT promoter and represses telomerase expression, possibly by interfering with MYC binding. 
Sequence Annotation
 REGION 380 532 Necessary for interaction with NEK2.
 MOD_RES 16 16 Phosphoserine.
 MOD_RES 61 61 N6-acetyllysine.
 MOD_RES 214 214 Phosphoserine.
 MOD_RES 428 428 Phosphoserine.  
Keyword
 3D-structure; Acetylation; Cell cycle; Cell division; Centromere; Chromosome; Coiled coil; Complete proteome; Cytoplasm; Cytoskeleton; Disease mutation; Kinetochore; Mitosis; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 718 AA 
Protein Sequence
MEDLGENTMV LSTLRSLNNF ISQRVEGGSG LDISTSAPGS LQMQYQQSMQ LEERAEQIRS 60
KSHLIQVERE KMQMELSHKR ARVELERAAS TSARNYEREV DRNQELLTRI RQLQEREAGA 120
EEKMQEQLER NRQCQQNLDA ASKRLREKED SLAQAGETIN ALKGRISELQ WSVMDQEMRV 180
KRLESEKQEL QEQLDLQHKK CQEANQKIQE LQASQEARAD HEQQIKDLEQ KLSLQEQDAA 240
IVKNMKSELV RLPRLERELK QLREESAHLR EMRETNGLLQ EELEGLQRKL GRQEKMQETL 300
VGLELENERL LAKLQSWERL DQTMGLSIRT PEDLSRFVVE LQQRELALKD KNSAVTSSAR 360
GLEKARQQLQ EELRQVSGQL LEERKKRETH EALARRLQKR VLLLTKERDG MRAILGSYDS 420
ELTPAEYSPQ LTRRMREAED MVQKVHSHSA EMEAQLSQAL EELGGQKQRA DMLEMELKML 480
KSQSSSAEQS FLFSREEADT LRLKVEELEG ERSRLEEEKR MLEAQLERRA LQGDYDQSRT 540
KVLHMSLNPT SVARQRLRED HSQLQAECER LRGLLRAMER GGTVPADLEA AAASLPSSKE 600
VAELKKQVES AELKNQRLKE VFQTKIQEFR KACYTLTGYQ IDITTENQYR LTSLYAEHPG 660
DCLIFKATSP SGSKMQLLET EFSHTVGELI EVHLRRQDSI PAFLSSLTLE LFSRQTVA 718 
Gene Ontology
 GO:0015629; C:actin cytoskeleton; IDA:HPA.
 GO:0005813; C:centrosome; NAS:UniProtKB.
 GO:0000777; C:condensed chromosome kinetochore; IEA:UniProtKB-SubCell.
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0000776; C:kinetochore; IDA:UniProtKB.
 GO:0005739; C:mitochondrion; IDA:HPA.
 GO:0072686; C:mitotic spindle; IDA:UniProtKB.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0000090; P:mitotic anaphase; NAS:UniProtKB.
 GO:0000278; P:mitotic cell cycle; TAS:Reactome.
 GO:0000089; P:mitotic metaphase; NAS:UniProtKB.
 GO:0007094; P:mitotic spindle assembly checkpoint; TAS:Reactome.
 GO:0000093; P:mitotic telophase; NAS:UniProtKB.
 GO:0090235; P:regulation of metaphase plate congression; IDA:UniProtKB. 
Interpro
 IPR008672; Mad1. 
Pfam
 PF05557; MAD 
SMART
  
PROSITE
  
PRINTS