UniProt Accession

 DDAH2_HUMAN; O95865; A2BEZ7 

Genbank Protein ID

 AF070667; AJ012008; AF087894; AF129756; BA000025; AL662899; AL670886; AL844216; BX248244; CR354443; CR759787; CR936239; CH471081; BC001435 

Genbank Nucleotide ID

 AAD20973.1; CAB46079.1; AAP97193.1; AAD18074.1; BAB63377.1; CAI18413.1; CAI17820.1; CAM45772.1; CAM26098.1; CAQ07022.1; CAQ10110.1; CAQ08895.1; EAX03500.1; AAH01435.1 

Protein Name

 N(G),N(G)-dimethylarginine dimethylaminohydrolase 2 

Protein Synonyms/Alias

 DDAH-2; Dimethylarginine dimethylaminohydrolase 2; DDAHII; Dimethylargininase-2; Protein G6a; S-phase protein 

Gene Name

 DDAH2 

Gene Synonyms/Alias

 DDAH; G6A; NG30 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
105	PEVDGVRKALQDLGL	ubiquitination	[1, 2]
194	GSSDAAQKAVRAMAV	ubiquitination	[2, 3]

Reference

 [1] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724] 

Functional Description

 Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation. 

Sequence Annotation

 ACT_SITE 171 171 Proton donor (By similarity).
 ACT_SITE 276 276 Nucleophile (Potential).  

Keyword

 Complete proteome; Cytoplasm; Direct protein sequencing; Hydrolase; Mitochondrion; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 285 AA 

Protein Sequence

Gene Ontology

 GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
 GO:0016597; F:amino acid binding; IEA:Compara.
 GO:0016403; F:dimethylargininase activity; IDA:UniProtKB.
 GO:0006527; P:arginine catabolic process; TAS:ProtInc.
 GO:0000052; P:citrulline metabolic process; IDA:UniProtKB.
 GO:0043066; P:negative regulation of apoptotic process; TAS:UniProtKB.
 GO:0006809; P:nitric oxide biosynthetic process; TAS:ProtInc.
 GO:0007263; P:nitric oxide mediated signal transduction; TAS:ProtInc.
 GO:0045429; P:positive regulation of nitric oxide biosynthetic process; ISS:BHF-UCL. 

Interpro

 IPR003198; Amidino_trans. 

Pfam

 PF02274; Amidinotransf 

SMART

  

PROSITE

  

PRINTS