CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-032137
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Eukaryotic peptide chain release factor subunit 1 
Protein Synonyms/Alias
 cDNA FLJ56175, highly similar to Eukaryotic peptide chain release factor subunit1 
Gene Name
 ETF1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
157NTREVLHKFTVDLPKubiquitination[1, 2]
338YLTPEQEKDKSHFTDacetylation[3]
370WFANNYKKFGATLEIubiquitination[1]
381TLEIVTDKSQEGSQFubiquitination[2, 4, 5]
390QEGSQFVKGFGGIGGubiquitination[1, 4, 5, 6]
Reference
 [1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377]
 [4] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [5] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 423 AA 
Protein Sequence
MKQDVLNCTE GPIHSNGTSM ISLIIPPKDQ ISRVAKMLAD EFGTASNIKS RVNRLSVLGA 60
ITSVQQRLKL YNKVPPNGLV VYCGTIVTEE GKEKKVNIDF EPFKPINTSL YLCDNKFHTE 120
ALTALLSDDS KFGFIVIDGS GALFGTLQGN TREVLHKFTV DLPKKHGRGG QSALRFARLR 180
MEKRHNYVRK VAETAVQLFI SGDKVNVAGL VLAGSADFKT ELSQSDMFDQ RLQSKVLKLV 240
DISYGGENGF NQAIELSTEV LSNVKFIQEK KLIGRYFDEI SQDTGKYCFG VEDTLKALEM 300
GAVEILIVYE NLDIMRYVLH CQGTEEEKIL YLTPEQEKDK SHFTDKETGQ EHELIESMPL 360
LEWFANNYKK FGATLEIVTD KSQEGSQFVK GFGGIGGILR YRVDFQGMEY QGGDDEFFDL 420
DDY 423 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:InterPro.
 GO:0016149; F:translation release factor activity, codon specific; IEA:InterPro. 
Interpro
 IPR005140; eRF1_1_Pelota.
 IPR005141; eRF1_2.
 IPR005142; eRF1_3.
 IPR004403; Peptide_chain-rel_eRF1/aRF1.
 IPR024049; Release_factor_eRF1/aRF1_N. 
Pfam
 PF03463; eRF1_1
 PF03464; eRF1_2
 PF03465; eRF1_3 
SMART
  
PROSITE
  
PRINTS