CPLM-011338

Genbank Nucleotide ID

Nucleoside diphosphate kinase A

Protein Synonyms/Alias

NDK A; NDP kinase A; Metastasis inhibition factor NM23; Tumor metastatic process-associated protein

Rattus norvegicus (Rat)

Position	Peptide	Type	References
49	QASEDLLKEHYIDLK	acetylation	[1]
49	QASEDLLKEHYIDLK	ubiquitination	[2]
56	KEHYIDLKDRPFFSG	acetylation	[1]

[1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
[2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113]

Functional Description

Major role in the synthesis of nucleoside triphosphates other than ATP. The ATP gamma phosphate is transferred to the NDP beta phosphate via a ping-pong mechanism, using a phosphorylated active-site intermediate. Possesses nucleoside-diphosphate kinase, serine/threonine-specific protein kinase, geranyl and farnesyl pyrophosphate kinase, histidine protein kinase and 3'-5' exonuclease activities. Involved in cell proliferation, differentiation and development, signal transduction, G protein- coupled receptor endocytosis, and gene expression. Required for neural development including neural patterning and cell fate determination (By similarity).

ACT_SITE 118 118 Pros-phosphohistidine intermediate (By
BINDING 12 12 ATP (By similarity).
BINDING 60 60 ATP (By similarity).
BINDING 88 88 ATP (By similarity).
BINDING 94 94 ATP (By similarity).
BINDING 105 105 ATP (By similarity).
BINDING 115 115 ATP (By similarity).
MOD_RES 2 2 N-acetylalanine (By similarity).
CROSSLNK 100 100 Glycyl lysine isopeptide (Lys-Gly)

Acetylation; ATP-binding; Complete proteome; Cytoplasm; Differentiation; Direct protein sequencing; Endocytosis; Isopeptide bond; Kinase; Magnesium; Metal-binding; Neurogenesis; Nucleotide metabolism; Nucleotide-binding; Nucleus; Reference proteome; Transferase; Ubl conjugation.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005813; C:centrosome; IDA:RGD.
GO:0005829; C:cytosol; IDA:RGD.
GO:0016020; C:membrane; IDA:RGD.
GO:0005739; C:mitochondrion; IEA:Compara.
GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
GO:0048471; C:perinuclear region of cytoplasm; IDA:RGD.
GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
GO:0004536; F:deoxyribonuclease activity; IEA:Compara.
GO:0043015; F:gamma-tubulin binding; IDA:RGD.
GO:0005525; F:GTP binding; IEA:Compara.
GO:0000287; F:magnesium ion binding; IEA:Compara.
GO:0004550; F:nucleoside diphosphate kinase activity; IDA:RGD.
GO:0000977; F:RNA polymerase II regulatory region sequence-specific DNA binding; IDA:RGD.
GO:0003697; F:single-stranded DNA binding; IDA:RGD.
GO:0035690; P:cellular response to drug; IEP:RGD.
GO:0071398; P:cellular response to fatty acid; IEP:RGD.
GO:0071333; P:cellular response to glucose stimulus; IEP:RGD.
GO:0006241; P:CTP biosynthetic process; IEA:InterPro.
GO:0006897; P:endocytosis; IEA:UniProtKB-KW.
GO:0006183; P:GTP biosynthetic process; IEA:InterPro.
GO:0021766; P:hippocampus development; IEP:RGD.
GO:0007595; P:lactation; IEA:Compara.
GO:0010629; P:negative regulation of gene expression; IDA:RGD.
GO:0002762; P:negative regulation of myeloid leukocyte differentiation; IDA:RGD.
GO:0043388; P:positive regulation of DNA binding; IEA:Compara.
GO:0050679; P:positive regulation of epithelial cell proliferation; IEA:Compara.
GO:0010976; P:positive regulation of neuron projection development; IMP:RGD.
GO:0014075; P:response to amine stimulus; IEP:RGD.
GO:0051591; P:response to cAMP; IEP:RGD.
GO:0033574; P:response to testosterone stimulus; IEP:RGD.
GO:0006228; P:UTP biosynthetic process; IEA:InterPro.

IPR001564; Nucleoside_diP_kinase.
IPR023005; Nucleoside_diP_kinase_AS.

PS00469; NDP_KINASES

PR01243; NUCDPKINASE.