UniProt Accession

 OFUT2_MOUSE; Q8VHI3; Q8VEM2; Q9CV66 

Genbank Protein ID

 AF455270; BC018194; AK009301 

Genbank Nucleotide ID

 AAL65192.1; BAB26202.1 

Protein Name

 GDP-fucose protein O-fucosyltransferase 2 

Protein Synonyms/Alias

 Peptide-O-fucosyltransferase 2; O-FucT-2 

Gene Name

 Pofut2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
71	IRVASLLKTLLKTEE	acetylation	[1]
171	PLLYSQDKHEYYRGW	acetylation	[1]
417	RFCGDQEKACEQPTH	acetylation	[1]

Reference

 [1] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441] 

Functional Description

 Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type I repeats (TSRs) where C1 and C2 are the first and second cysteines of the repeat, respectively. O-fucosylates members of several protein families including the ADAMTS superfamily and the thrombosporin (TSP) and spondin families. Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13 (By similarity). O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm. 

Sequence Annotation

 REGION 53 56 Substrate binding (By similarity).
 REGION 388 389 Substrate binding (By similarity).
 ACT_SITE 54 54 Proton donor/acceptor (By similarity).
 BINDING 294 294 Substrate (By similarity).
 CARBOHYD 189 189 N-linked (GlcNAc...) (Potential).
 CARBOHYD 209 209 N-linked (GlcNAc...) (Potential).
 CARBOHYD 259 259 N-linked (GlcNAc...) (Potential).
 DISULFID 161 192 By similarity.
 DISULFID 412 419 By similarity.  

Keyword

 Carbohydrate metabolism; Complete proteome; Disulfide bond; Endoplasmic reticulum; Fucose metabolism; Glycoprotein; Glycosyltransferase; Golgi apparatus; Reference proteome; Signal; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 429 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IEA:UniProtKB-SubCell.
 GO:0005794; C:Golgi apparatus; IEA:UniProtKB-SubCell.
 GO:0046922; F:peptide-O-fucosyltransferase activity; ISS:UniProtKB.
 GO:0006004; P:fucose metabolic process; IEA:UniProtKB-KW.
 GO:0001707; P:mesoderm formation; IMP:MGI.
 GO:0010717; P:regulation of epithelial to mesenchymal transition; IMP:MGI.
 GO:0010468; P:regulation of gene expression; IMP:MGI.
 GO:0051046; P:regulation of secretion; IEA:Compara. 

Interpro

 IPR019378; GDP-Fuc_O-FucTrfase. 

Pfam

 PF10250; O-FucT 

SMART

  

PROSITE

  

PRINTS