CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-000885
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Putative pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 
Protein Synonyms/Alias
 ATP-dependent RNA helicase #3; DEAH-box protein 16 
Gene Name
 DHX16 
Gene Synonyms/Alias
 DBP2; DDX16; KIAA0577 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
202NVLERSDKKAYEEAQacetylation[1]
203VLERSDKKAYEEAQKacetylation[1]
291RAAGEQEKLEATNRYubiquitination[2]
340RLGAASLKFGARDAAubiquitination[2, 3]
389TSTQAQQKESIQAVRubiquitination[2]
447GYTNKGMKIACTQPRubiquitination[2]
537DILFGLIKDVARFRPubiquitination[2]
716LTVTPCSKASANQRAubiquitination[2, 4, 5]
733AGRVAAGKCFRLYTAubiquitination[2]
814ELTTSGRKMAELPVDubiquitination[2]
Reference
 [1] Regulation of cellular metabolism by protein lysine acetylation.
 Zhao S, Xu W, Jiang W, Yu W, Lin Y, Zhang T, Yao J, Zhou L, Zeng Y, Li H, Li Y, Shi J, An W, Hancock SM, He F, Qin L, Chin J, Yang P, Chen X, Lei Q, Xiong Y, Guan KL.
 Science. 2010 Feb 19;327(5968):1000-4. [PMID: 20167786]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [4] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302
Functional Description
 Probable ATP-binding RNA helicase involved in pre-mRNA splicing. 
Sequence Annotation
 DOMAIN 409 573 Helicase ATP-binding.
 DOMAIN 598 771 Helicase C-terminal.
 NP_BIND 422 429 ATP (By similarity).
 MOTIF 520 523 DEAH box.
 MOD_RES 103 103 Phosphoserine.
 MOD_RES 106 106 Phosphoserine.
 MOD_RES 107 107 Phosphoserine.
 MOD_RES 160 160 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Helicase; Hydrolase; mRNA processing; mRNA splicing; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1041 AA 
Protein Sequence
MATPAGLERW VQDELHSVLG LSERHVAQFL IGTAQRCTSA EEFVQRLRDT DTLDLSGPAR 60
DFALRLWNKV PRKAVVEKPA RAAEREARAL LEKNRSYRLL EDSEESSEET VSRAGSSLQK 120
KRKKRKHLRK KREEEEEEEA SEKGKKKTGG SKQQTEKPES EDEWERTERE RLQDLEERDA 180
FAERVRQRDK DRTRNVLERS DKKAYEEAQK RLKMAEEDRK AMVPELRKKS RREYLAKRER 240
EKLEDLEAEL ADEEFLFGDV ELSRHERQEL KYKRRVRDLA REYRAAGEQE KLEATNRYHM 300
PKETRGQPAR AVDLVEEESG APGEEQRRWE EARLGAASLK FGARDAASQE PKYQLVLEEE 360
ETIEFVRATQ LQGDEEPSAP PTSTQAQQKE SIQAVRRSLP VFPFREELLA AIANHQVLII 420
EGETGSGKTT QIPQYLFEEG YTNKGMKIAC TQPRRVAAMS VAARVAREMG VKLGNEVGYS 480
IRFEDCTSER TVLRYMTDGM LLREFLSEPD LASYSVVMVD EAHERTLHTD ILFGLIKDVA 540
RFRPELKVLV ASATMDTARF STFFDDAPVF RIPGRRFPVD IFYTKAPEAD YLEACVVSVL 600
QIHVTQPPGD ILVFLTGQEE IEAACEMLQD RCRRLGSKIR ELLVLPIYAN LPSDMQARIF 660
QPTPPGARKV VVATNIAETS LTIEGIIYVL DPGFCKQKSY NPRTGMESLT VTPCSKASAN 720
QRAGRAGRVA AGKCFRLYTA WAYQHELEET TVPEIQRTSL GNVVLLLKSL GIHDLMHFDF 780
LDPPPYETLL LALEQLYALG ALNHLGELTT SGRKMAELPV DPMLSKMILA SEKYSCSEEI 840
LTVAAMLSVN NSIFYRPKDK VVHADNARVN FFLPGGDHLV LLNVYTQWAE SGYSSQWCYE 900
NFVQFRSMRR ARDVREQLEG LLERVEVGLS SCQGDYIRVR KAITAGYFYH TARLTRSGYR 960
TVKQQQTVFI HPNSSLFEQQ PRWLLYHELV LTTKEFMRQV LEIESSWLLE VAPHYYKAKE 1020
LEDPHAKKMP KKIGKTREEL G 1041 
Gene Ontology
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0008026; F:ATP-dependent helicase activity; IEA:InterPro.
 GO:0003676; F:nucleic acid binding; IEA:InterPro.
 GO:0003724; F:RNA helicase activity; TAS:ProtInc.
 GO:0006397; P:mRNA processing; IEA:UniProtKB-KW.
 GO:0008380; P:RNA splicing; TAS:ProtInc. 
Interpro
 IPR011545; DNA/RNA_helicase_DEAD/DEAH_N.
 IPR002464; DNA/RNA_helicase_DEAH_CS.
 IPR011709; DUF1605.
 IPR007502; Helicase-assoc_dom.
 IPR014001; Helicase_ATP-bd.
 IPR001650; Helicase_C.
 IPR027417; P-loop_NTPase. 
Pfam
 PF00270; DEAD
 PF04408; HA2
 PF00271; Helicase_C
 PF07717; OB_NTP_bind 
SMART
 SM00487; DEXDc
 SM00847; HA2
 SM00490; HELICc 
PROSITE
 PS00690; DEAH_ATP_HELICASE
 PS51192; HELICASE_ATP_BIND_1
 PS51194; HELICASE_CTER 
PRINTS