CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-009184
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Actin 
Protein Synonyms/Alias
  
Gene Name
 ACT1 
Gene Synonyms/Alias
 ABY1; END7; YFL039C 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
50IMVGMGQKDSYVGDEacetylation[1]
50IMVGMGQKDSYVGDEubiquitination[2, 3]
61VGDEAQSKRGILTLRubiquitination[3]
84TNWDDMEKIWHHTFYubiquitination[3]
113TEAPMNPKSNREKMTacetylation[1]
113TEAPMNPKSNREKMTubiquitination[3]
191DLTDYLMKILSERGYubiquitination[2, 3]
315GIAERMQKEITALAPubiquitination[3]
326ALAPSSMKVKIIAPPubiquitination[3]
328APSSMKVKIIAPPERacetylation[1]
328APSSMKVKIIAPPERubiquitination[3]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [3] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. 
Sequence Annotation
 MOD_RES 1 1 N-acetylmethionine.  
Keyword
 3D-structure; Acetylation; ATP-binding; Complete proteome; Cytoplasm; Cytoskeleton; Direct protein sequencing; Nucleotide-binding; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 375 AA 
Protein Sequence
MDSEVAALVI DNGSGMCKAG FAGDDAPRAV FPSIVGRPRH QGIMVGMGQK DSYVGDEAQS 60
KRGILTLRYP IEHGIVTNWD DMEKIWHHTF YNELRVAPEE HPVLLTEAPM NPKSNREKMT 120
QIMFETFNVP AFYVSIQAVL SLYSSGRTTG IVLDSGDGVT HVVPIYAGFS LPHAILRIDL 180
AGRDLTDYLM KILSERGYSF STTAEREIVR DIKEKLCYVA LDFEQEMQTA AQSSSIEKSY 240
ELPDGQVITI GNERFRAPEA LFHPSVLGLE SAGIDQTTYN SIMKCDVDVR KELYGNIVMS 300
GGTTMFPGIA ERMQKEITAL APSSMKVKII APPERKYSVW IGGSILASLT TFQQMWISKQ 360
EYDESGPSIV HHKCF 375 
Gene Ontology
 GO:0030479; C:actin cortical patch; IDA:SGD.
 GO:0005884; C:actin filament; IDA:SGD.
 GO:0032432; C:actin filament bundle; IDA:SGD.
 GO:0000142; C:cellular bud neck contractile ring; IDA:SGD.
 GO:0031011; C:Ino80 complex; IPI:SGD.
 GO:0035267; C:NuA4 histone acetyltransferase complex; IPI:SGD.
 GO:0000812; C:Swr1 complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0005200; F:structural constituent of cytoskeleton; IDA:SGD.
 GO:0000916; P:actomyosin contractile ring contraction; IDA:SGD.
 GO:0030476; P:ascospore wall assembly; IDA:SGD.
 GO:0007119; P:budding cell isotropic bud growth; TAS:SGD.
 GO:0034599; P:cellular response to oxidative stress; IGI:SGD.
 GO:0001300; P:chronological cell aging; IMP:SGD.
 GO:0006281; P:DNA repair; IDA:SGD.
 GO:0006897; P:endocytosis; IMP:SGD.
 GO:0000132; P:establishment of mitotic spindle orientation; TAS:SGD.
 GO:0006887; P:exocytosis; TAS:SGD.
 GO:0016573; P:histone acetylation; IDA:SGD.
 GO:0000001; P:mitochondrion inheritance; TAS:SGD.
 GO:0009306; P:protein secretion; IMP:SGD.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; IDA:SGD.
 GO:0000011; P:vacuole inheritance; IGI:SGD.
 GO:0030050; P:vesicle transport along actin filament; TAS:SGD. 
Interpro
 IPR004000; Actin-related.
 IPR020902; Actin/actin-like_CS.
 IPR004001; Actin_CS. 
Pfam
 PF00022; Actin 
SMART
 SM00268; ACTIN 
PROSITE
 PS00406; ACTINS_1
 PS00432; ACTINS_2
 PS01132; ACTINS_ACT_LIKE 
PRINTS
 PR00190; ACTIN.