UniProt Accession

 PI42A_HUMAN; P48426; B0YJ66; D3DRV1; P53807; Q5VUX3 

Genbank Protein ID

 U14957; S78798; AL513128; AL157707; AL390318; EF445009; EF445009; AL157707; AL390318; AL513128; AL390318; AL157707; AL513128; CH471072; CH471072; BC018034 

Genbank Nucleotide ID

 AAA64835.2; AAB35041.1; CAH72211.1; CAH72211.1; CAH72211.1; ACA06044.1; ACA06045.1; CAI39585.1; CAI39585.1; CAI39585.1; CAH70526.1; CAH70526.1; CAH70526.1; EAW86140.1; EAW86141.1; AAH18034.1 

Protein Name

 Phosphatidylinositol 5-phosphate 4-kinase type-2 alpha 

Protein Synonyms/Alias

 1-phosphatidylinositol 5-phosphate 4-kinase 2-alpha; Diphosphoinositide kinase 2-alpha; PIP5KIII; Phosphatidylinositol 5-phosphate 4-kinase type II alpha; PI(5)P 4-kinase type II alpha; PIP4KII-alpha; PtdIns(4)P-5-kinase B isoform; PtdIns(4)P-5-kinase C isoform; PtdIns(5)P-4-kinase isoform 2-alpha 

Gene Name

 PIP4K2A 

Gene Synonyms/Alias

 PIP5K2; PIP5K2A 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
15	GSSVLASKTKTKKKH	ubiquitination	[1]
89	ENMPSHFKFKEYCPM	acetylation	[2]
91	MPSHFKFKEYCPMVF	ubiquitination	[1]
145	YDKRYIIKTITSEDV	acetylation	[2]
213	VYRKYDLKGSTVARE	ubiquitination	[1]
252	IYIDDNNKKVFLEKL	ubiquitination	[1]
258	NKKVFLEKLKKDVEF	ubiquitination	[1]
260	KVFLEKLKKDVEFLA	ubiquitination	[1]
395	VNPEQYSKRFLDFIG	ubiquitination	[3, 4]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861]
 [3] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [4] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
 Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
 J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931] 

Functional Description

 Catalyzes the phosphorylation of phosphatidylinositol 5- phosphate (PtdIns5P) on the fourth hydroxyl of the myo-inositol ring, to form phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2). May exert its function by regulating the levels of PtdIns5P, which functions in the cytosol by increasing AKT activity and in the nucleus signals through ING2. May regulate the pool of cytosolic PtdIns5P in response to the activation of tyrosine phosphorylation. May negatively regulate insulin- stimulated glucose uptake by lowering the levels of PtdIns5P. May be involved in thrombopoiesis, and the terminal maturation of megakaryocytes and regulation of their size. 

Sequence Annotation

 DOMAIN 33 405 PIPK.
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 3 3 Phosphothreonine.
 MOD_RES 14 14 Phosphoserine.
 MOD_RES 89 89 N6-acetyllysine.
 MOD_RES 145 145 N6-acetyllysine.  

Keyword

 3D-structure; Acetylation; ATP-binding; Cell membrane; Complete proteome; Cytoplasm; Direct protein sequencing; Kinase; Membrane; Nucleotide-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 406 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0016308; F:1-phosphatidylinositol-4-phosphate 5-kinase activity; NAS:UniProtKB.
 GO:0016309; F:1-phosphatidylinositol-5-phosphate 4-kinase activity; IEA:EC.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0035855; P:megakaryocyte development; IEA:Compara.
 GO:0006661; P:phosphatidylinositol biosynthetic process; TAS:Reactome.
 GO:0044281; P:small molecule metabolic process; TAS:Reactome. 

Interpro

 IPR023610; PInositol-4-P-5-kinase.
 IPR027483; PInositol-4-P-5-kinase_C.
 IPR002498; PInositol-4-P-5-kinase_core.
 IPR027484; PInositol-4-P-5-kinase_N.
 IPR016034; PInositol-4P-5-kinase_core_sub. 

Pfam

 PF01504; PIP5K 

SMART

 SM00330; PIPKc 

PROSITE

 PS51455; PIPK 

PRINTS