UniProt Accession

 NNF1_YEAST; P47149; D6VWT1; Q06779 

Genbank Protein ID

 X93350; Z49612; AY558256; BK006943 

Genbank Nucleotide ID

 CAA63725.1; CAA89642.1; AAS56582.1; DAA08897.1 

Protein Name

 Kinetochore-associated protein NNF1 

Protein Synonyms/Alias

  

Gene Name

 NNF1 

Gene Synonyms/Alias

 YJR112W; J2011 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
44	FPQYVNSKQGAINVA	ubiquitination	[1]

Reference

 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 

Functional Description

 Acts as essential component of the kinetochore MIND complex, which is required for the spindle checkpoint and kinetochore integrity. MIND plays a role in establishing a bipolar spindle-kinetochore interaction by joining kinetochore subunits contacting DNA to those contacting microtubules. NNF1 is required for a number of nuclear functions. Cells depleted of NNF11 or containing a temperature-sensitive NNF1 mutation have elongated microtubules and become bi- and multinucleate. They also have a fragmented nucleolus and accumulate poly(A)+ RNA inside the nucleus. 

Sequence Annotation

  

Keyword

 Cell cycle; Cell division; Centromere; Chromosome; Complete proteome; Kinetochore; Mitosis; Nucleus; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 201 AA 

Protein Sequence

Gene Ontology

 GO:0000818; C:nuclear MIS12/MIND complex; IDA:SGD.
 GO:0051301; P:cell division; IEA:UniProtKB-KW.
 GO:0007059; P:chromosome segregation; IDA:SGD.
 GO:0007067; P:mitosis; IEA:UniProtKB-KW. 

Interpro

 IPR016851; Kinetochore-assoc_Nnf1p.
 IPR007128; Nnf1. 

Pfam

 PF03980; Nnf1 

SMART

  

PROSITE

  

PRINTS