UniProt Accession

 RISB_ECOLI; P61714; P25540; P77114; Q2MC11 

Genbank Protein ID

 X64395; U82664; U00096; AP009048 

Genbank Nucleotide ID

 CAA45736.1; AAB40171.1; AAC73518.1; BAE76195.1 

Protein Name

 6,7-dimethyl-8-ribityllumazine synthase 

Protein Synonyms/Alias

 DMRL synthase; LS; Lumazine synthase 

Gene Name

 ribE 

Gene Synonyms/Alias

 ribH; ybaF; b0415; JW0405 

Created Date

 July 27, 2013 

Organism

 Escherichia coli (strain K12) 

NCBI Taxa ID

 83333 

Lysine Modification

Position	Peptide	Type	References
39	EGAIDALKRIGQVKD	acetylation	[1]
45	LKRIGQVKDENITVV	acetylation	[1]
72	GALAKTGKYDAVIAL	acetylation	[1]
132	AIERAGTKAGNKGAE	acetylation	[1]
136	AGTKAGNKGAEAALT	acetylation	[1]
152	LEMINVLKAIKA***	acetylation	[1]

Reference

 [1] Acetyl-Phosphate Is a Critical Determinant of Lysine Acetylation in E. coli.
 Weinert BT, Iesmantavicius V, Wagner SA, Schölz C, Gummesson B, Beli P, Nyström T, Choudhary C.
 Mol Cell. 2013 Jul 25;51(2):265-72. [PMID: 23830618] 

Functional Description

 Catalyzes the formation of 6,7-dimethyl-8- ribityllumazine by condensation of 5-amino-6-(D- ribitylamino)uracil with 3,4-dihydroxy-2-butanone 4-phosphate. This is the penultimate step in the biosynthesis of riboflavin. 

Sequence Annotation

 REGION 57 59 5-amino-6-(D-ribitylamino)uracil binding
 REGION 81 83 5-amino-6-(D-ribitylamino)uracil binding
 REGION 86 87 1-deoxy-L-glycero-tetrulose 4-phosphate
 ACT_SITE 89 89 Proton donor (Potential).
 BINDING 22 22 5-amino-6-(D-ribitylamino)uracil (By
 BINDING 114 114 5-amino-6-(D-ribitylamino)uracil; via
 BINDING 128 128 1-deoxy-L-glycero-tetrulose 4-phosphate  

Keyword

 Complete proteome; Reference proteome; Riboflavin biosynthesis; Transferase. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 156 AA 

Protein Sequence

Gene Ontology

 GO:0009349; C:riboflavin synthase complex; IEA:InterPro.
 GO:0000906; F:6,7-dimethyl-8-ribityllumazine synthase activity; IDA:EcoCyc.
 GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
 GO:0009231; P:riboflavin biosynthetic process; IEA:HAMAP. 

Interpro

 IPR002180; DMRL_synthase. 

Pfam

 PF00885; DMRL_synthase 

SMART

  

PROSITE

  

PRINTS