CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023114
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 F-box/WD repeat-containing protein 2 
Protein Synonyms/Alias
 F-box and WD-40 domain-containing protein 2; Protein MD6 
Gene Name
 FBXW2 
Gene Synonyms/Alias
 FBW2; FWD2 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
131LKAILRMKQLEDHEAubiquitination[1]
298YILLSADKYEIKIWPacetylation[2]
313IGREINCKCLKTLSVubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 
Functional Description
 Substrate-recognition component of the SCF (SKP1-CUL1-F- box protein)-type E3 ubiquitin ligase complex. 
Sequence Annotation
 DOMAIN 54 101 F-box.
 REPEAT 146 183 WD 1.
 REPEAT 185 221 WD 2.
 REPEAT 224 265 WD 3.
 REPEAT 276 314 WD 4.
 MOD_RES 298 298 N6-acetyllysine.  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Reference proteome; Repeat; Ubl conjugation pathway; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 454 AA 
Protein Sequence
MERKDFETWL DNISVTFLSL TDLQKNETLD HLISLSGAVQ LRHLSNNLET LLKRDFLKLL 60
PLELSFYLLK WLDPQTLLTC CLVSKQWNKV ISACTEVWQT ACKNLGWQID DSVQDALHWK 120
KVYLKAILRM KQLEDHEAFE TSSLIGHSAR VYALYYKDGL LCTGSDDLSA KLWDVSTGQC 180
VYGIQTHTCA AVKFDEQKLV TGSFDNTVAC WEWSSGARTQ HFRGHTGAVF SVDYNDELDI 240
LVSGSADFTV KVWALSAGTC LNTLTGHTEW VTKVVLQKCK VKSLLHSPGD YILLSADKYE 300
IKIWPIGREI NCKCLKTLSV SEDRSICLQP RLHFDGKYIV CSSALGLYQW DFASYDILRV 360
IKTPEIANLA LLGFGDIFAL LFDNRYLYIM DLRTESLISR WPLPEYRKSK RGSSFLAGEA 420
SWLNGLDGHN DTGLVFATSM PDHSIHLVLW KEHG 454 
Gene Ontology
 GO:0005737; C:cytoplasm; IEA:Compara.
 GO:0004842; F:ubiquitin-protein ligase activity; TAS:ProtInc.
 GO:0006508; P:proteolysis; TAS:ProtInc. 
Interpro
 IPR001810; F-box_dom_cyclin-like.
 IPR020472; G-protein_beta_WD-40_rep.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF00646; F-box
 PF00400; WD40 
SMART
 SM00256; FBOX
 SM00320; WD40 
PROSITE
 PS50181; FBOX
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS
 PR00320; GPROTEINBRPT.