UniProt Accession

 F5GX24_HUMAN; F5GX24 

Genbank Protein ID

 AL117355 

Genbank Nucleotide ID

  

Protein Name

 G2/M phase-specific E3 ubiquitin-protein ligase 

Protein Synonyms/Alias

  

Gene Name

 G2E3 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
40	LKCCVCKKNGASIGC	ubiquitination	[1, 2]
120	ILRSPCCKNAWFHRD	ubiquitination	[1]
154	NNSDIFQKEMLRMGI	ubiquitination	[1]
206	DYNAPDSKWEIKRCQ	ubiquitination	[1]
210	PDSKWEIKRCQCCGS	ubiquitination	[1]
251	YNSGEFQKAKKHVLP	ubiquitination	[1]
253	SGEFQKAKKHVLPNS	ubiquitination	[2]
276	LLEESSPKLPRQSPG	ubiquitination	[1, 2]
287	QSPGSQSKDLLRQGS	ubiquitination	[1, 2]
396	SLNSQALKENLYYEA	ubiquitination	[3]
525	KTLGVLEKIQAYPEA	ubiquitination	[1]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473] 

Functional Description

  

Sequence Annotation

  

Keyword

 Complete proteome; Ligase; Metal-binding; Reference proteome; Ubl conjugation pathway; Zinc; Zinc-finger. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 660 AA 

Protein Sequence

Gene Ontology

 GO:0005794; C:Golgi apparatus; IDA:HPA.
 GO:0004842; F:ubiquitin-protein ligase activity; IEA:Compara.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0001824; P:blastocyst development; IEA:Compara.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0000209; P:protein polyubiquitination; IEA:Compara. 

Interpro

 IPR000569; HECT.
 IPR019786; Zinc_finger_PHD-type_CS.
 IPR011011; Znf_FYVE_PHD.
 IPR001965; Znf_PHD.
 IPR001841; Znf_RING.
 IPR013083; Znf_RING/FYVE/PHD. 

Pfam

 PF00632; HECT 

SMART

 SM00249; PHD
 SM00184; RING 

PROSITE

 PS50237; HECT
 PS01359; ZF_PHD_1 

PRINTS