CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010720
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Rho GTPase-activating protein 4 
Protein Synonyms/Alias
 Rho-GAP hematopoietic protein C1; Rho-type GTPase-activating protein 4; p115 
Gene Name
 ARHGAP4 
Gene Synonyms/Alias
 KIAA0131; RGC1; RHOGAP4 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
22AEYETQVKEMRWQLSubiquitination[1]
69EYSRGLEKLAERFSSubiquitination[1]
92REHQSFRKEPSLLSPubiquitination[1]
169SELQTAKKTYQAYHMubiquitination[1]
184ESVNAEAKLREAERQubiquitination[1]
276QAKFMEHKLKCTKARubiquitination[1]
278KFMEHKLKCTKARNEubiquitination[1]
421IETEEVNKTLKATLQubiquitination[1]
483TETFYLTKLQEYLSGubiquitination[1, 2]
496SGRSILAKLQAKHEKubiquitination[1]
503KLQAKHEKLQEALQRubiquitination[1]
513EALQRGDKEEQEVSWubiquitination[1]
551LFGGDMEKFIQSSGQubiquitination[1]
838MRGLIPHKYITLPAGubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Inhibitory effect on stress fiber organization. May down-regulate Rho-like GTPase in hematopoietic cells. 
Sequence Annotation
 DOMAIN 15 88 FCH.
 DOMAIN 507 695 Rho-GAP.
 DOMAIN 746 805 SH3.  
Keyword
 3D-structure; Alternative splicing; Coiled coil; Complete proteome; Cytoplasm; GTPase activation; Polymorphism; Reference proteome; SH3 domain. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 946 AA 
Protein Sequence
MAAHGKLRRE RGLQAEYETQ VKEMRWQLSE QLRCLELQGE LRRELLQELA EFMRRRAEVE 60
LEYSRGLEKL AERFSSRGGR LGSSREHQSF RKEPSLLSPL HCWAVLLQHT RQQSRESAAL 120
SEVLAGPLAQ RLSHIAEDVG RLVKKSRDLE QQLQDELLEV VSELQTAKKT YQAYHMESVN 180
AEAKLREAER QEEKRAGRSV PTTTAGATEA GPLRKSSLKK GGRLVEKLWP PQRPVAASSC 240
APVCWLQAGF LVHPPWWGAM CAPSTHQRQA KFMEHKLKCT KARNEYLLSL ASVNAAVSNY 300
YLHDVLDLMD CCDTGFHLAL GQVLRSYTAA ESRTQASQVQ GLGSLEEAVE ALDPPGDKAK 360
VLEVHATVFC PPLRFDYHPH DGDEVAEICV EMELRDEILP RAQNIQSRLD RQTIETEEVN 420
KTLKATLQAL LEVVASDDGD VLDSFQTSPS TESLKSTSSD PGSRQAGRRR GQQQETETFY 480
LTKLQEYLSG RSILAKLQAK HEKLQEALQR GDKEEQEVSW TQYTQRKFQK SRQPRPSSQY 540
NQRLFGGDME KFIQSSGQPV PLVVESCIRF INLNGLQHEG IFRVSGAQLR VSEIRDAFER 600
GEDPLVEGCT AHDLDSVAGV LKLYFRSLEP PLFPPDLFGE LLASSELEAT AERVEHVSRL 660
LWRLPAPVLV VLRYLFTFLN HLAQYSDENM MDPYNLAVCF GPTLLPVPAG QDPVALQGRV 720
NQLVQTLIVQ PDRVFPPLTS LPGPVYEKCM APPSASCLGD AQLESLGADN EPELEAEMPA 780
QEDDLEGVVE AVACFAYTGR TAQELSFRRG DVLRLHERAS SDWWRGEHNG MRGLIPHKYI 840
TLPAGTEKQV VGAGLQTAGE SGSSPEGLLA SELVHRPEPC TSPEAMGPSG HRRRCLVPAS 900
PEQHVEVDKA VAQNMDSVFK ELLGKTSVRQ GLGPASTTSP SPGPRSPKAP PSSRLGRNKG 960
FSRGPGAPAS PSASHPQGLD TTPKPH 986 
Gene Ontology
 GO:0005829; C:cytosol; TAS:Reactome.
 GO:0005925; C:focal adhesion; IDA:HPA.
 GO:0005634; C:nucleus; IDA:HPA.
 GO:0005100; F:Rho GTPase activator activity; TAS:ProtInc.
 GO:0005070; F:SH3/SH2 adaptor activity; TAS:ProtInc.
 GO:0097190; P:apoptotic signaling pathway; TAS:Reactome.
 GO:0007010; P:cytoskeleton organization; TAS:ProtInc.
 GO:0048011; P:neurotrophin TRK receptor signaling pathway; TAS:Reactome.
 GO:0007266; P:Rho protein signal transduction; TAS:ProtInc. 
Interpro
 IPR001060; FCH_dom.
 IPR008936; Rho_GTPase_activation_prot.
 IPR000198; RhoGAP_dom.
 IPR001452; SH3_domain. 
Pfam
 PF00611; FCH
 PF00620; RhoGAP
 PF00018; SH3_1 
SMART
 SM00055; FCH
 SM00324; RhoGAP
 SM00326; SH3 
PROSITE
 PS50133; FCH
 PS50238; RHOGAP
 PS50002; SH3 
PRINTS