CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-024010
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 TAF6-like RNA polymerase II p300/CBP-associated factor-associated factor 65 kDa subunit 6L 
Protein Synonyms/Alias
 PCAF-associated factor 65-alpha; PAF65-alpha 
Gene Name
 TAF6L 
Gene Synonyms/Alias
 PAF65A 
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
62MKHTKRRKLTVEDFNubiquitination[1]
137HVSYLDGKGNLAPQGubiquitination[1]
177GDDPQLMKVALQDLQubiquitination[1, 2, 3]
373VERLLKMKAQAAEPNubiquitination[1]
549GTRDVFQKSRFAPRGubiquitination[1, 4]
597PASRYVQKLPMIGRTubiquitination[1]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Functions as a component of the PCAF complex. The PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. The PCAF complex could be considered as the human version of the yeast SAGA complex. 
Sequence Annotation
  
Keyword
 Complete proteome; Direct protein sequencing; Nucleus; Reference proteome; Transcription; Transcription regulation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 622 AA 
Protein Sequence
MSEREERRFV EIPRESVRLM AESTGLELSD EVAALLAEDV CYRLREATQN SSQFMKHTKR 60
RKLTVEDFNR ALRWSSVEAV CGYGSQEALP MRPAREGELY FPEDREVNLV ELALATNIPK 120
GCAETAVRVH VSYLDGKGNL APQGSVPSAV SSLTDDLLKY YHQVTRAVLG DDPQLMKVAL 180
QDLQTNSKIG ALLPYFVYVV SGVKSVSHDL EQLHRLLQVA RSLFRNPHLC LGPYVRCLVG 240
SVLYCVLEPL AASINPLNDH WTLRDGAALL LSHIFWTHGD LVSGLYQHIL LSLQKILADP 300
VRPLCCHYGA VVGLHALGWK AVERVLYPHL STYWTNLQAV LDDYSVSNAQ VKADGHKVYG 360
AILVAVERLL KMKAQAAEPN RGGPGGRGCR RLDDLPWDSL LFQESSSGGG AEPSFGSGLP 420
LPPGGAGPED PSLSVTLADI YRELYAFFGD SLATRFGTGQ PAPTAPRPPG DKKEPAAAPD 480
SVRKMPQLTA SAIVSPHGDE SPRGSGGGGP ASASGPAASE SRPLPRVHRA RGAPRQQGPG 540
TGTRDVFQKS RFAPRGAPHF RFIIAGRQAG RRCRGRLFQT AFPAPYGPSP ASRYVQKLPM 600
IGRTSRPARR WALSDYSLYL PL 622 
Gene Ontology
 GO:0000118; C:histone deacetylase complex; TAS:ProtInc.
 GO:0030914; C:STAGA complex; IDA:UniProtKB.
 GO:0003677; F:DNA binding; TAS:ProtInc.
 GO:0003713; F:transcription coactivator activity; IDA:UniProtKB.
 GO:0006338; P:chromatin remodeling; TAS:ProtInc.
 GO:0006352; P:DNA-dependent transcription, initiation; IEA:InterPro.
 GO:0043966; P:histone H3 acetylation; IDA:UniProtKB.
 GO:0051090; P:regulation of sequence-specific DNA binding transcription factor activity; IEA:InterPro.
 GO:0006357; P:regulation of transcription from RNA polymerase II promoter; TAS:ProtInc. 
Interpro
 IPR011442; DUF1546.
 IPR009072; Histone-fold.
 IPR004823; TAF_TATA-bd. 
Pfam
 PF07571; DUF1546
 PF02969; TAF 
SMART
 SM00803; TAF 
PROSITE
  
PRINTS