CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-004297
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Glutamine synthetase 
Protein Synonyms/Alias
 GS; Glutamate decarboxylase; Glutamate--ammonia ligase 
Gene Name
 Glul 
Gene Synonyms/Alias
 Glns 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
11SASSHLNKGIKQMYMubiquitination[1]
14SHLNKGIKQMYMSLPubiquitination[1]
91MFRDPFRKDPNKLVLacetylation[2]
91MFRDPFRKDPNKLVLubiquitination[1]
95PFRKDPNKLVLCEVFubiquitination[1]
103LVLCEVFKYNRKPAEubiquitination[1]
107EVFKYNRKPAETNLRubiquitination[1]
118TNLRHICKRIMDMVSacetylation[2]
268MREENGLKCIEEAIDacetylation[3]
268MREENGLKCIEEAIDubiquitination[1]
276CIEEAIDKLSKRHQYubiquitination[1]
291HIRAYDPKGGLDNARubiquitination[1]
333PRTVGQEKKGYFEDRacetylation[2, 4]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [3] Quantitative assessment of the impact of the gut microbiota on lysine epsilon-acetylation of host proteins using gnotobiotic mice.
 Simon GM, Cheng J, Gordon JI.
 Proc Natl Acad Sci U S A. 2012 Jul 10;109(28):11133-8. [PMID: 22733758]
 [4] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654] 
Functional Description
 Essential for proliferation of fetal skin fibroblasts. This enzyme has 2 functions: it catalyzes the production of glutamine and 4-aminobutanoate (gamma-aminobutyric acid, GABA), the latter in a pyridoxal phosphate-independent manner (By similarity). 
Sequence Annotation
 MOD_RES 2 2 N-acetylalanine.
 MOD_RES 104 104 Phosphotyrosine.
 MOD_RES 180 180 Phosphotyrosine.  
Keyword
 Acetylation; ATP-binding; Complete proteome; Cytoplasm; Direct protein sequencing; Ligase; Lyase; Mitochondrion; Nucleotide-binding; Phosphoprotein; Reference proteome; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 373 AA 
Protein Sequence
MATSASSHLN KGIKQMYMSL PQGEKVQAMY IWVDGTGEGL RCKTRTLDCE PKCVEELPEW 60
NFDGSSTFQS EGSNSDMYLH PVAMFRDPFR KDPNKLVLCE VFKYNRKPAE TNLRHICKRI 120
MDMVSNQHPW FGMEQEYTLM GTDGHPFGWP SNGFPGPQGP YYCGVGADKA YGRDIVEAHY 180
RACLYAGVKI TGTNAEVMPA QWEFQIGPCE GIRMGDHLWI ARFILHRVCE DFGVIATFDP 240
KPIPGNWNGA GCHTNFSTKA MREENGLKCI EEAIDKLSKR HQYHIRAYDP KGGLDNARRL 300
TGFHETSNIN DFSAGVANRG ASIRIPRTVG QEKKGYFEDR RPSANCDPYA VTEAIVRTCL 360
LNETGDEPFQ YKN 373 
Gene Ontology
 GO:0043679; C:axon terminus; IEA:Compara.
 GO:0005739; C:mitochondrion; IDA:MGI.
 GO:0043204; C:perikaryon; IEA:Compara.
 GO:0043234; C:protein complex; IEA:Compara.
 GO:0005791; C:rough endoplasmic reticulum; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0016595; F:glutamate binding; IEA:Compara.
 GO:0004351; F:glutamate decarboxylase activity; IEA:EC.
 GO:0004356; F:glutamate-ammonia ligase activity; IEA:EC.
 GO:0000287; F:magnesium ion binding; IEA:Compara.
 GO:0030145; F:manganese ion binding; IEA:Compara.
 GO:0008283; P:cell proliferation; IEA:Compara.
 GO:0006536; P:glutamate metabolic process; IEA:Compara.
 GO:0006542; P:glutamine biosynthetic process; IEA:Compara.
 GO:0050679; P:positive regulation of epithelial cell proliferation; IEA:Compara.
 GO:0032024; P:positive regulation of insulin secretion; IEA:Compara.
 GO:0051968; P:positive regulation of synaptic transmission, glutamatergic; IEA:Compara.
 GO:0051260; P:protein homooligomerization; IEA:Compara.
 GO:0009749; P:response to glucose stimulus; IMP:MGI. 
Interpro
 IPR008147; Gln_synt_beta.
 IPR014746; Gln_synth/guanido_kin_cat_dom.
 IPR008146; Gln_synth_cat_dom.
 IPR027303; Gln_synth_gly_rich_site.
 IPR027302; Gln_synth_N_conserv_site. 
Pfam
 PF00120; Gln-synt_C
 PF03951; Gln-synt_N 
SMART
  
PROSITE
 PS00180; GLNA_1
 PS00181; GLNA_ATP 
PRINTS