UniProt Accession

 ARL2_MOUSE; Q9D0J4; Q9WUM1 

Genbank Protein ID

 AF143680; AK011366; BC060259 

Genbank Nucleotide ID

 AAD33908.1; BAB27572.1; AAH60259.1 

Protein Name

 ADP-ribosylation factor-like protein 2 

Protein Synonyms/Alias

  

Gene Name

 Arl2 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
53	PTLGFNIKTLEHRGF	ubiquitination	[1]
61	TLEHRGFKLNIWDVG	ubiquitination	[1]
71	IWDVGGQKSLRSYWR	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Small GTP-binding protein which cycles between an inactive GDP-bound and an active GTP-bound form, and the rate of cycling is regulated by guanine nucleotide exchange factors (GEF) and GTPase-activating proteins (GAP). GTP-binding protein that does not act as an allosteric activator of the cholera toxin catalytic subunit. Regulates formation of new microtubules and centrosome integrity. Prevents the TBCD-induced microtubule destruction. Participates in association with TBCD, in the disassembly of the apical junction complexes. Antagonizes the effect of TBCD on epithelial cell detachment and tight and adherens junctions disassembly. Together with ARL2, plays a role in the nuclear translocation, retention and transcriptional activity of STAT3. Component of a regulated secretory pathway involved in Ca(2+)-dependent release of acetylcholine. Required for normal progress through the cell cycle. 

Sequence Annotation

 NP_BIND 23 30 GTP.
 NP_BIND 66 70 GTP.
 NP_BIND 125 128 GTP.
 BINDING 68 68 GTP; via amide nitrogen.
 MOD_RES 45 45 Phosphoserine (By similarity).
 LIPID 2 2 N-myristoyl glycine (Potential).
 CROSSLNK 71 71 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 3D-structure; Cell cycle; Complete proteome; Cytoplasm; Cytoskeleton; GTP-binding; Isopeptide bond; Lipoprotein; Mitochondrion; Myristate; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome; Ubl conjugation. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 184 AA 

Protein Sequence

Gene Ontology

 GO:0005813; C:centrosome; ISS:UniProtKB.
 GO:0005737; C:cytoplasm; ISS:UniProtKB.
 GO:0016328; C:lateral plasma membrane; ISS:UniProtKB.
 GO:0005758; C:mitochondrial intermembrane space; IEA:UniProtKB-SubCell.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0019003; F:GDP binding; IDA:UniProtKB.
 GO:0005525; F:GTP binding; IDA:UniProtKB.
 GO:0003924; F:GTPase activity; ISS:UniProtKB.
 GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
 GO:0051297; P:centrosome organization; ISS:UniProtKB.
 GO:0051457; P:maintenance of protein location in nucleus; ISS:UniProtKB.
 GO:0034260; P:negative regulation of GTPase activity; ISS:UniProtKB.
 GO:0010811; P:positive regulation of cell-substrate adhesion; ISS:UniProtKB.
 GO:0031116; P:positive regulation of microtubule polymerization; ISS:UniProtKB.
 GO:0007264; P:small GTPase mediated signal transduction; IEA:InterPro.
 GO:0070830; P:tight junction assembly; ISS:UniProtKB. 

Interpro

 IPR027417; P-loop_NTPase.
 IPR005225; Small_GTP-bd_dom.
 IPR024156; Small_GTPase_ARF.
 IPR006689; Small_GTPase_ARF/SAR. 

Pfam

 PF00025; Arf 

SMART

 SM00177; ARF 

PROSITE

 PS51417; ARF 

PRINTS

 PR00328; SAR1GTPBP.