CPLM-020222

Genbank Nucleotide ID

Vesicle-associated membrane protein 8

Protein Synonyms/Alias

VAMP-8; Endobrevin; EDB

Homo sapiens (Human)

Position	Peptide	Type	References
24	QSEVEGVKNIMTQNV	ubiquitination	[1, 2, 3, 4, 5]
47	NLEHLRNKTEDLEAT	ubiquitination	[1, 2, 3, 4, 5, 6]
59	EATSEHFKTTSQKVA	ubiquitination	[1, 2, 5]
64	HFKTTSQKVARKFWW	ubiquitination	[1, 2, 5]

[1] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Ubiquitin ligase substrate identification through quantitative proteomics at both the protein and peptide levels.
Lee KA, Hammerle LP, Andrews PS, Stokes MP, Mustelin T, Silva JC, Black RA, Doedens JR.
J Biol Chem. 2011 Dec 2;286(48):41530-8. [PMID: 21987572]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
Chen Z, Zhou Y, Song J, Zhang Z.
Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
[6] Proteome-wide identification of ubiquitylation sites by conjugation of engineered lysine-less ubiquitin.
Oshikawa K, Matsumoto M, Oyamada K, Nakayama KI.
J Proteome Res. 2012 Feb 3;11(2):796-807. [PMID: 22053931]

Functional Description

SNAREs, Soluble N-ethylmaleimide-sensitive factor- attachment protein receptors, are essential proteins for fusion of cellular membranes. SNAREs localized on opposing membranes assemble to form a trans-SNARE complex, an extended, parallel four alpha-helical bundle that drives membrane fusion. VAMP8 is a SNARE involved in autophagy through the direct control of autophagosome membrane fusion with the lysososome membrane. Also required for dense-granule secretion in platelets. Plays also a role in regulated enzyme secretion in pancreatic acinar cells. Involved in the abscission of the midbody during cell division, which leads to completely separate daughter cells. Involved in the homotypic fusion of early and late endosomes.

DOMAIN 12 72 v-SNARE coiled-coil homology.
MOD_RES 1 1 N-acetylmethionine.
MOD_RES 18 18 Phosphoserine (By similarity).

Acetylation; Autophagy; Coiled coil; Complete proteome; Direct protein sequencing; Endosome; Lysosome; Membrane; Phosphoprotein; Protein transport; Reference proteome; Transmembrane; Transmembrane helix; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005769; C:early endosome; TAS:ProtInc.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0031902; C:late endosome membrane; IDA:UniProtKB.
GO:0005765; C:lysosomal membrane; IDA:UniProtKB.
GO:0005739; C:mitochondrion; IEA:Compara.
GO:0005886; C:plasma membrane; TAS:Reactome.
GO:0055037; C:recycling endosome; IEA:Compara.
GO:0030667; C:secretory granule membrane; TAS:Reactome.
GO:0031201; C:SNARE complex; IDA:UniProtKB.
GO:0000046; P:autophagic vacuole fusion; IMP:UniProtKB.
GO:0006892; P:post-Golgi vesicle-mediated transport; TAS:Reactome.
GO:0006461; P:protein complex assembly; IEA:Compara.
GO:0015031; P:protein transport; IEA:UniProtKB-KW.
GO:0030100; P:regulation of endocytosis; IEA:Compara.
GO:0006906; P:vesicle fusion; IEA:Compara.

IPR001388; Synaptobrevin.
IPR016444; Synaptobrevin_met/fun.

PF00957; Synaptobrevin

PS00417; SYNAPTOBREVIN
PS50892; V_SNARE

PR00219; SYNAPTOBREVN.