CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-023526
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Ubiquitin carboxyl-terminal hydrolase isozyme L5 
Protein Synonyms/Alias
 UCH-L5; Ubiquitin C-terminal hydrolase UCH37; Ubiquitin thioesterase L5 
Gene Name
 Uchl5 
Gene Synonyms/Alias
 Uch37 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
45LEPESFEKLKPVHGLubiquitination[1]
47PESFEKLKPVHGLIFacetylation[2]
47PESFEKLKPVHGLIFsuccinylation[2]
154QMFEFDTKTPAKEEDubiquitination[1]
158FDTKTPAKEEDAFHFacetylation[2, 3, 4]
158FDTKTPAKEEDAFHFubiquitination[1]
206AVRPVIEKRIQKYSEubiquitination[1]
234RKMIYEQKIAELQRQubiquitination[1]
277LIEEEVQKLKRYKIEubiquitination[1]
282VQKLKRYKIENIRRKubiquitination[1]
289KIENIRRKHNYLPFIacetylation[2]
289KIENIRRKHNYLPFIsuccinylation[2]
315QLIPLVEKAKEKQNAubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [2] SIRT5-Mediated Lysine Desuccinylation Impacts Diverse Metabolic Pathways.
 Park J, Chen Y, Tishkoff DX, Peng C, Tan M, Dai L, Xie Z, Zhang Y, Zwaans BM, Skinner ME, Lombard DB, Zhao Y.
 Mol Cell. 2013 Jun 27;50(6):919-30. [PMID: 23806337]
 [3] Quantitative acetylome analysis reveals the roles of SIRT1 in regulating diverse substrates and cellular pathways.
 Chen Y, Zhao W, Yang JS, Cheng Z, Luo H, Lu Z, Tan M, Gu W, Zhao Y.
 Mol Cell Proteomics. 2012 Oct;11(10):1048-62. [PMID: 22826441]
 [4] Proteomic investigations of lysine acetylation identify diverse substrates of mitochondrial deacetylase sirt3.
 Sol EM, Wagner SA, Weinert BT, Kumar A, Kim HS, Deng CX, Choudhary C.
 PLoS One. 2012;7(12):e50545. [PMID: 23236377] 
Functional Description
 Protease that specifically cleaves 'Lys-48'-linked polyubiquitin chains. Deubiquitinating enzyme associated with the 19S regulatory subunit of the 26S proteasome. Putative regulatory component of the INO80 complex; however is inactive in the INO80 complex and is activated by a transient interaction of the INO80 complex with the proteasome via ADRM1 (By similarity). 
Sequence Annotation
 REGION 313 329 Interaction with ADRM1 (By similarity).
 ACT_SITE 88 88 Nucleophile (By similarity).
 ACT_SITE 164 164 Proton donor (By similarity).
 MOD_RES 158 158 N6-acetyllysine (By similarity).  
Keyword
 Acetylation; Alternative splicing; Complete proteome; Cytoplasm; DNA damage; DNA recombination; DNA repair; Hydrolase; Nucleus; Protease; Proteasome; Reference proteome; Thiol protease; Transcription; Transcription regulation; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 329 AA 
Protein Sequence
MSSNAGEWCL MESDPGVFTE LIKGFGCRGA QVEEIWSLEP ESFEKLKPVH GLIFLFKWQP 60
GEEPAGSVVQ DSRLETIFFA KQVINNACAT QAIVSVLLNC THQDVHLGET LSEFKEFSQS 120
FDAAMKGLAL SNSDVIRQVH NSFARQQMFE FDTKTPAKEE DAFHFVSYVP VNGRLYELDG 180
LREGPIDLGA CNQDDWITAV RPVIEKRIQK YSEGEIRFNL MAIVSDRKMI YEQKIAELQR 240
QLAEEEPMDT DQGSTVLSAI QSEVARNQML IEEEVQKLKR YKIENIRRKH NYLPFIMELL 300
KTLAEHQQLI PLVEKAKEKQ NAKKAQETK 329 
Gene Ontology
 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0031011; C:Ino80 complex; IEA:Compara.
 GO:0005634; C:nucleus; ISS:UniProtKB.
 GO:0000502; C:proteasome complex; IEA:UniProtKB-KW.
 GO:0004866; F:endopeptidase inhibitor activity; IEA:Compara.
 GO:0008242; F:omega peptidase activity; IEA:InterPro.
 GO:0004221; F:ubiquitin thiolesterase activity; TAS:Reactome.
 GO:0004843; F:ubiquitin-specific protease activity; ISS:UniProtKB.
 GO:0006310; P:DNA recombination; IEA:UniProtKB-KW.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:0048853; P:forebrain morphogenesis; IMP:BHF-UCL.
 GO:0021670; P:lateral ventricle development; IMP:BHF-UCL.
 GO:0030901; P:midbrain development; IMP:BHF-UCL.
 GO:0016579; P:protein deubiquitination; ISS:UniProtKB.
 GO:0061136; P:regulation of proteasomal protein catabolic process; IEA:Compara.
 GO:0006355; P:regulation of transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro. 
Interpro
 IPR001578; Peptidase_C12.
 IPR017390; Ubiquitinyl_hydrolase_UCH37. 
Pfam
 PF01088; Peptidase_C12 
SMART
  
PROSITE
 PS00140; UCH_1 
PRINTS
 PR00707; UBCTHYDRLASE.