UniProt Accession

 LRC59_HUMAN; Q96AG4; B2RE83; D3DTX8; Q9P189 

Genbank Protein ID

 CH471109; CH471109; BC017168; BC052279; AK316593; AF119857 

Genbank Nucleotide ID

 EAW94615.1; EAW94616.1; AAH17168.1; AAH52279.1; BAG38180.1; AAF69611.1 

Protein Name

 Leucine-rich repeat-containing protein 59 

Protein Synonyms/Alias

 Ribosome-binding protein p34; p34 

Gene Name

 LRRC59 

Gene Synonyms/Alias

 PRO1855 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
14	KGGNLRDKLDGNELD	ubiquitination	[1]
33	DLNEVPVKELAALPK	ubiquitination	[1, 2]
40	KELAALPKATILDLS	ubiquitination	[3, 4, 5, 6]
66	CGLTHLVKLDLSKNK	ubiquitination	[3, 5, 7]
71	LVKLDLSKNKLQQLP	acetylation	[6]
73	KLDLSKNKLQQLPAD	acetylation	[6]
73	KLDLSKNKLQQLPAD	ubiquitination	[1, 3, 4, 5, 6, 7]
96	HLDLLNNKLVTLPVS	ubiquitination	[2, 8]
108	PVSFAQLKNLKWLDL	ubiquitination	[2, 8, 9]
111	FAQLKNLKWLDLKDN	acetylation	[10]
111	FAQLKNLKWLDLKDN	ubiquitination	[1, 3, 5, 7]
116	NLKWLDLKDNPLDPV	ubiquitination	[1, 2, 6, 7]
126	PLDPVLAKVAGDCLD	ubiquitination	[8]
135	AGDCLDEKQCKQCAN	acetylation	[6]
135	AGDCLDEKQCKQCAN	ubiquitination	[7, 8]
149	NKVLQHMKAVQADQE	ubiquitination	[1, 7, 8]
207	RKEYDALKAAKREQE	ubiquitination	[7]

Reference

 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [5] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [6] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [7] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [10] Lysine acetylation targets protein complexes and co-regulates major cellular functions.
 Choudhary C, Kumar C, Gnad F, Nielsen ML, Rehman M, Walther TC, Olsen JV, Mann M.
 Science. 2009 Aug 14;325(5942):834-40. [PMID: 19608861] 

Functional Description

 Required for nuclear import of FGF1, but not that of FGF2. Might regulate nuclear import of exogenous FGF1 by facilitating interaction with the nuclear import machinery and by transporting cytosolic FGF1 to, and possibly through, the nuclear pores. 

Sequence Annotation

 REPEAT 10 31 LRR 1.
 REPEAT 40 62 LRR 2.
 REPEAT 63 84 LRR 3.
 REPEAT 86 107 LRR 4.
 REPEAT 109 128 LRR 5.
 MOD_RES 23 23 Phosphoserine.
 MOD_RES 25 25 Phosphoserine.  

Keyword

 Coiled coil; Complete proteome; Direct protein sequencing; Endoplasmic reticulum; Leucine-rich repeat; Membrane; Microsome; Nucleus; Phosphoprotein; Reference proteome; Repeat; Signal-anchor; Transmembrane; Transmembrane helix. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 307 AA 

Protein Sequence

Gene Ontology

 GO:0005783; C:endoplasmic reticulum; IDA:HPA.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0042645; C:mitochondrial nucleoid; IDA:BHF-UCL.
 GO:0005635; C:nuclear envelope; IEA:UniProtKB-SubCell. 

Interpro

 IPR001611; Leu-rich_rpt. 

Pfam

 PF00560; LRR_1 

SMART

  

PROSITE

 PS51450; LRR 

PRINTS