CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-002454
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Mitochondrial import receptor subunit TOM70 
Protein Synonyms/Alias
 70 kDa mitochondrial outer membrane protein; Translocase of outer membrane 70 kDa subunit 
Gene Name
 TOM70 
Gene Synonyms/Alias
 MAS70; OMP1; YNL121C; N1905 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
162STKALELKPDYSKVLacetylation[1]
167ELKPDYSKVLLRRASacetylation[1]
167ELKPDYSKVLLRRASubiquitination[2]
213MLERNLNKQAMSKLKubiquitination[2]
242ELSTQPAKERKDKQEubiquitination[2]
391NYFDKALKLDSNNSSacetylation[1]
533EATNLLEKASKLDPRacetylation[1]
536NLLEKASKLDPRSEQubiquitination[2]
610KIQETLAKLREQGLMubiquitination[2, 3]
Reference
 [1] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [2] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [3] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047] 
Functional Description
 Component of the TOM (translocase of outer membrane) receptor complex responsible for the recognition and translocation of cytosolically synthesized mitochondrial preproteins. Together with TOM20 and TOM22 functions as the transit peptide receptor at the surface of the mitochondrion outer membrane and facilitates the movement of preproteins into the TOM40 translocation pore. 
Sequence Annotation
 REPEAT 99 132 TPR 1.
 REPEAT 134 165 TPR 2.
 REPEAT 281 315 TPR 3.
 REPEAT 363 396 TPR 4.
 REPEAT 397 430 TPR 5.
 REPEAT 432 464 TPR 6.
 REPEAT 465 498 TPR 7.
 REPEAT 505 541 TPR 8.
 REPEAT 542 575 TPR 9.
 MOD_RES 174 174 Phosphoserine.  
Keyword
 3D-structure; Complete proteome; Membrane; Mitochondrion; Mitochondrion outer membrane; Phosphoprotein; Reference proteome; Repeat; TPR repeat; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 617 AA 
Protein Sequence
MKSFITRNKT AILATVAATG TAIGAYYYYN QLQQQQQRGK KNTINKDEKK DTKDSQKETE 60
GAKKSTAPSN PPIYPVSSNG EPDFSNKANF TAEEKDKYAL ALKDKGNQFF RNKKYDDAIK 120
YYNWALELKE DPVFYSNLSA CYVSVGDLKK VVEMSTKALE LKPDYSKVLL RRASANEGLG 180
KFADAMFDLS VLSLNGDFND ASIEPMLERN LNKQAMSKLK EKFGDIDTAT ATPTELSTQP 240
AKERKDKQEN LPSVTSMASF FGIFKPELTF ANYDESNEAD KELMNGLSNL YKRSPESYDK 300
ADESFTKAAR LFEEQLDKNN EDEKLKEKLA ISLEHTGIFK FLKNDPLGAH EDIKKAIELF 360
PRVNSYIYMA LIMADRNDST EYYNYFDKAL KLDSNNSSVY YHRGQMNFIL QNYDQAGKDF 420
DKAKELDPEN IFPYIQLACL AYRENKFDDC ETLFSEAKRK FPEAPEVPNF FAEILTDKND 480
FDKALKQYDL AIELENKLDG IYVGIAPLVG KATLLTRNPT VENFIEATNL LEKASKLDPR 540
SEQAKIGLAQ MKLQQEDIDE AITLFEESAD LARTMEEKLQ AITFAEAAKV QQRIRSDPVL 600
AKKIQETLAK LREQGLM 617 
Gene Ontology
 GO:0031307; C:integral to mitochondrial outer membrane; IDA:SGD.
 GO:0005742; C:mitochondrial outer membrane translocase complex; IDA:SGD.
 GO:0030943; F:mitochondrion targeting sequence binding; IMP:SGD.
 GO:0015450; F:P-P-bond-hydrolysis-driven protein transmembrane transporter activity; IEA:InterPro.
 GO:0045039; P:protein import into mitochondrial inner membrane; IDA:SGD.
 GO:0030150; P:protein import into mitochondrial matrix; IMP:SGD. 
Interpro
 IPR005687; Tom70.
 IPR001440; TPR-1.
 IPR013026; TPR-contain_dom.
 IPR011990; TPR-like_helical.
 IPR019734; TPR_repeat. 
Pfam
 PF00515; TPR_1 
SMART
 SM00028; TPR 
PROSITE
 PS50005; TPR
 PS50293; TPR_REGION 
PRINTS