CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-021743
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Electrogenic sodium bicarbonate cotransporter 1 
Protein Synonyms/Alias
 Sodium bicarbonate cotransporter; NBC-like protein; Na(+)/HCO3(-) cotransporter; Solute carrier family 4 member 4 
Gene Name
 Slc4a4 
Gene Synonyms/Alias
 Nbc; Nbc1; Nbce1; Rnbc1 
Created Date
 July 27, 2013 
Organism
 Rattus norvegicus (Rat) 
NCBI Taxa ID
 10116 
Lysine Modification
Position
Peptide
Type
References
67ISENYSDKSDVENADacetylation[1]
82ESSSSILKPLISPAAacetylation[1]
137RWIKFEEKVEQGGERacetylation[1]
203LKPDLKDKVTYTLLRubiquitination[2]
229RSLADIGKTVSSASRubiquitination[2]
264SLNDISDKPEKDQLKacetylation[1]
448RFCGGLIKDIKRKAPacetylation[1]
676LSKKECVKFGGKLVGacetylation[1]
760LIVPSEFKPTSPHRGacetylation[1]
1062QPFLSDNKPLDRERSacetylation[1]
Reference
 [1] Proteomic analysis of lysine acetylation sites in rat tissues reveals organ specificity and subcellular patterns.
 Lundby A, Lage K, Weinert BT, Bekker-Jensen DB, Secher A, Skovgaard T, Kelstrup CD, Dmytriyev A, Choudhary C, Lundby C, Olsen JV.
 Cell Rep. 2012 Aug 30;2(2):419-31. [PMID: 22902405]
 [2] Synaptic protein ubiquitination in rat brain revealed by antibody-based ubiquitome analysis.
 Na CH, Jones DR, Yang Y, Wang X, Xu Y, Peng J.
 J Proteome Res. 2012 Sep 7;11(9):4722-32. [PMID: 22871113] 
Functional Description
 Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH. 
Sequence Annotation
 REGION 748 779 Interaction with CA4 (By similarity).
 REGION 1002 1004 CA2-binding (By similarity).
 REGION 1030 1033 CA2-binding (By similarity).
 REGION 1057 1059 Required for basolateral targeting (By
 MOD_RES 30 30 Phosphotyrosine (By similarity).
 MOD_RES 254 254 Phosphothreonine (By similarity).
 MOD_RES 255 255 Phosphoserine (By similarity).
 MOD_RES 257 257 Phosphoserine (By similarity).
 MOD_RES 262 262 Phosphoserine (By similarity).
 MOD_RES 1026 1026 Phosphoserine; by PKA (By similarity).
 MOD_RES 1029 1029 Phosphoserine (By similarity).
 MOD_RES 1034 1034 Phosphoserine (By similarity).
 CARBOHYD 641 641 N-linked (GlcNAc...).
 CARBOHYD 661 661 N-linked (GlcNAc...).  
Keyword
 Alternative splicing; Cell membrane; Complete proteome; Glycoprotein; Ion transport; Membrane; Phosphoprotein; Reference proteome; Sodium; Sodium transport; Symport; Transmembrane; Transmembrane helix; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1079 AA 
Protein Sequence
MEDEAVLDRG ASFLKHVCDE EEVEGHHTIY IGVHVPKSYR RRRRHKRKAG HKEKKEKERI 60
SENYSDKSDV ENADESSSSI LKPLISPAAE RIRFILGEED DSPAPPQLFT ELDELLAVDG 120
QEMEWKETAR WIKFEEKVEQ GGERWSKPHV ATLSLHSLFE LRTCMEKGSI MLDREASSLP 180
QLVEMIADHQ IETGLLKPDL KDKVTYTLLR KHRHQTKKSN LRSLADIGKT VSSASRMFSN 240
PDNGSPAMTH RNLTSSSLND ISDKPEKDQL KNKFMKKLPR DAEASNVLVG EVDFLDTPFI 300
AFVRLQQAVM LGALTEVPVP TRFLFILLGP KGKAKSYHEI GRAIATLMSD EVFHDIAYKA 360
KDRHDLIAGI DEFLDEVIVL PPGEWDPAIR IEPPKSLPSS DKRKNMYSGG ENVQMNGDTP 420
HDGGHGGGGH GDCEELQRTG RFCGGLIKDI KRKAPFFASD FYDALNIQAL SAILFIYLAT 480
VTNAITFGGL LGDATDNMQG VLESFLGTAV SGAIFCLFAG QPLTILSSTG PVLVFERLLF 540
NFSKDHSFDY LEFRLWIGLW SAFMCLILVA TDASFLVQYF TRFTEEGFSS LISFIFIYDA 600
FKKMIKLADY YPINSDFRVG YNTHFSCACL PPDPVNLSVS NDTTLAPEDL PTVSSTDMYH 660
NATFDWAYLS KKECVKFGGK LVGNNCDFVP DITLMSFILF LGTYTSSMAM KKFKTSRYFP 720
TTARKLISDF AIILSILIFC VIDALVGVDT PKLIVPSEFK PTSPHRGWFV PPFGGNPWWV 780
CLAAAIPALL VTILIFMDQQ ITAVIVNRKE HKLKKGAGYH LDLFWVAILM VVCSFMALPW 840
YVAATVISIA HIDSLKMETE TSAPGEQPKF LGVREQRVTG TLVFILTGLS VFMAPILKFI 900
PMPVLYGVFL YMGVASLNGV QFMDRLKLLL MPLKHQPDFI YLRHVPLRRV HLFTSLQVLC 960
LALLWILKST VAAIIFPVMI LALVAVRKGM DYLFSQHDLS FLDDVIPEKD KKKKEDEKKK 1020
KKKKGSLDSD NDDSDCPYSE KVPSIKIPMD ITEQQPFLSD NKPLDRERSS TFLERHTSC 1079 
Gene Ontology
 GO:0016323; C:basolateral plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005887; C:integral to plasma membrane; TAS:RGD.
 GO:0005452; F:inorganic anion exchanger activity; IEA:InterPro.
 GO:0008510; F:sodium:bicarbonate symporter activity; TAS:RGD.
 GO:0006885; P:regulation of pH; TAS:RGD. 
Interpro
 IPR013769; Band3_cytoplasmic_dom.
 IPR011531; HCO3_transpt_C.
 IPR003020; HCO3_transpt_euk.
 IPR003024; Na/HCO3_transpt.
 IPR016152; PTrfase/Anion_transptr. 
Pfam
 PF07565; Band_3_cyto
 PF00955; HCO3_cotransp 
SMART
  
PROSITE
  
PRINTS
 PR01231; HCO3TRNSPORT.
 PR01232; NAHCO3TRSPRT.