CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010813
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Tyrosine-protein kinase receptor UFO 
Protein Synonyms/Alias
 Adhesion-related kinase 
Gene Name
 Axl 
Gene Synonyms/Alias
 Ark; Ufo 
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
517LGISEELKEKLRDVMubiquitination[1]
534RHKVALGKTLGEGEFubiquitination[1]
763LRQGNRLKQPVDCLDubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 
Functional Description
 Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3- kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TENC1. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response. 
Sequence Annotation
 DOMAIN 30 122 Ig-like C2-type 1.
 DOMAIN 133 216 Ig-like C2-type 2.
 DOMAIN 219 326 Fibronectin type-III 1.
 DOMAIN 327 419 Fibronectin type-III 2.
 DOMAIN 530 801 Protein kinase.
 NP_BIND 536 544 ATP (By similarity).
 REGION 19 86 Interaction with GAS6 (By similarity).
 ACT_SITE 666 666 Proton acceptor (By similarity).
 BINDING 561 561 ATP (By similarity).
 MOD_RES 697 697 Phosphotyrosine; by autocatalysis (By
 MOD_RES 773 773 Phosphotyrosine; by autocatalysis (By
 MOD_RES 815 815 Phosphotyrosine; by autocatalysis (By
 MOD_RES 860 860 Phosphotyrosine; by autocatalysis (By
 CARBOHYD 37 37 N-linked (GlcNAc...) (Potential).
 CARBOHYD 151 151 N-linked (GlcNAc...) (Potential).
 CARBOHYD 192 192 N-linked (GlcNAc...) (Potential).
 CARBOHYD 333 333 N-linked (GlcNAc...) (Potential).
 CARBOHYD 339 339 N-linked (GlcNAc...) (Potential).
 CARBOHYD 395 395 N-linked (GlcNAc...) (Potential).
 DISULFID 50 111 By similarity.
 DISULFID 154 199 By similarity.  
Keyword
 ATP-binding; Cell membrane; Complete proteome; Differentiation; Disulfide bond; Glycoprotein; Immunity; Immunoglobulin domain; Innate immunity; Kinase; Membrane; Nucleotide-binding; Oncogene; Phosphoprotein; Receptor; Reference proteome; Repeat; Signal; Transferase; Transmembrane; Transmembrane helix; Tyrosine-protein kinase; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 888 AA 
Protein Sequence
MGRVPLAWWL ALCCWGCAAH KDTQTEAGSP FVGNPGNITG ARGLTGTLRC ELQVQGEPPE 60
VVWLRDGQIL ELADNTQTQV PLGEDWQDEW KVVSQLRISA LQLSDAGEYQ CMVHLEGRTF 120
VSQPGFVGLE GLPYFLEEPE DKAVPANTPF NLSCQAQGPP EPVTLLWLQD AVPLAPVTGH 180
SSQHSLQTPG LNKTSSFSCE AHNAKGVTTS RTATITVLPQ RPHHLHVVSR QPTELEVAWT 240
PGLSGIYPLT HCNLQAVLSD DGVGIWLGKS DPPEDPLTLQ VSVPPHQLRL EKLLPHTPYH 300
IRISCSSSQG PSPWTHWLPV ETTEGVPLGP PENVSAMRNG SQVLVRWQEP RVPLQGTLLG 360
YRLAYRGQDT PEVLMDIGLT REVTLELRGD RPVANLTVSV TAYTSAGDGP WSLPVPLEPW 420
RPGQGQPLHH LVSEPPPRAF SWPWWYVLLG ALVAAACVLI LALFLVHRRK KETRYGEVFE 480
PTVERGELVV RYRVRKSYSR RTTEATLNSL GISEELKEKL RDVMVDRHKV ALGKTLGEGE 540
FGAVMEGQLN QDDSILKVAV KTMKIAICTR SELEDFLSEA VCMKEFDHPN VMRLIGVCFQ 600
GSDREGFPEP VVILPFMKHG DLHSFLLYSR LGDQPVFLPT QMLVKFMADI ASGMEYLSTK 660
RFIHRDLAAR NCMLNENMSV CVADFGLSKK IYNGDYYRQG RIAKMPVKWI AIESLADRVY 720
TSKSDVWSFG VTMWEIATRG QTPYPGVENS EIYDYLRQGN RLKQPVDCLD GLYALMSRCW 780
ELNPRDRPSF AELREDLENT LKALPPAQEP DEILYVNMDE GGSHLEPRGA AGGADPPTQP 840
DPKDSCSCLT AADVHSAGRY VLCPSTAPGP TLSADRGCPA PPGQEDGA 888 
Gene Ontology
 GO:0009986; C:cell surface; IEA:Compara.
 GO:0005615; C:extracellular space; IEA:Compara.
 GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
 GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0001786; F:phosphatidylserine binding; IEA:Compara.
 GO:0004714; F:transmembrane receptor protein tyrosine kinase activity; IEA:EC.
 GO:0043277; P:apoptotic cell clearance; IGI:UniProtKB.
 GO:0001974; P:blood vessel remodeling; IMP:MGI.
 GO:0048469; P:cell maturation; IEA:Compara.
 GO:0031668; P:cellular response to extracellular stimulus; IDA:MGI.
 GO:0035457; P:cellular response to interferon-alpha; IEA:Compara.
 GO:0071222; P:cellular response to lipopolysaccharide; IEA:Compara.
 GO:0097028; P:dendritic cell differentiation; IEA:Compara.
 GO:0007167; P:enzyme linked receptor protein signaling pathway; IGI:MGI.
 GO:0034101; P:erythrocyte homeostasis; IMP:MGI.
 GO:0021885; P:forebrain cell migration; IGI:MGI.
 GO:0006954; P:inflammatory response; IMP:MGI.
 GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
 GO:0001779; P:natural killer cell differentiation; IGI:MGI.
 GO:2000669; P:negative regulation of dendritic cell apoptotic process; IEA:Compara.
 GO:0032689; P:negative regulation of interferon-gamma production; IEA:Compara.
 GO:0051250; P:negative regulation of lymphocyte activation; IGI:MGI.
 GO:0043524; P:negative regulation of neuron apoptotic process; IMP:MGI.
 GO:0032720; P:negative regulation of tumor necrosis factor production; IGI:MGI.
 GO:0001764; P:neuron migration; IMP:MGI.
 GO:0042698; P:ovulation cycle; IGI:MGI.
 GO:0030168; P:platelet activation; IMP:MGI.
 GO:0001961; P:positive regulation of cytokine-mediated signaling pathway; IEA:Compara.
 GO:0032825; P:positive regulation of natural killer cell differentiation; IEA:Compara.
 GO:0051897; P:positive regulation of protein kinase B signaling cascade; IMP:UniProtKB.
 GO:0043491; P:protein kinase B signaling cascade; IMP:MGI.
 GO:0032940; P:secretion by cell; IMP:MGI.
 GO:0007283; P:spermatogenesis; IGI:MGI.
 GO:0034446; P:substrate adhesion-dependent cell spreading; IMP:MGI.
 GO:0060068; P:vagina development; IGI:MGI. 
Interpro
 IPR003961; Fibronectin_type3.
 IPR007110; Ig-like_dom.
 IPR013783; Ig-like_fold.
 IPR003599; Ig_sub.
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
 IPR008266; Tyr_kinase_AS.
 IPR020635; Tyr_kinase_cat_dom. 
Pfam
 PF00041; fn3
 PF07714; Pkinase_Tyr 
SMART
 SM00060; FN3
 SM00409; IG
 SM00219; TyrKc 
PROSITE
 PS50853; FN3
 PS50835; IG_LIKE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00109; PROTEIN_KINASE_TYR 
PRINTS
 PR00109; TYRKINASE.