CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-018528
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Golgi reassembly-stacking protein 1 
Protein Synonyms/Alias
 Golgi peripheral membrane protein p65; Golgi reassembly-stacking protein of 65 kDa; GRASP65 
Gene Name
 Gorasp1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Mus musculus (Mouse) 
NCBI Taxa ID
 10090 
Lysine Modification
Position
Peptide
Type
References
55NKENDTLKALLKANVubiquitination[1]
Reference
 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023
Functional Description
 Stacking factor involved in the postmitotic assembly of Golgi stacks from mitotic Golgi fragments. Key structural protein required for the maintenance of the Golgi apparatus integrity: its caspase-mediated cleavage is required for fragmentation of the Golgi during apoptosis. Also mediates, via its interaction with GM130, the docking of transport vesicles with the Golgi membranes (By similarity). 
Sequence Annotation
 DOMAIN 5 74 PDZ.
 REGION 189 201 Essential for the interaction with GM130
 MOD_RES 224 224 Phosphothreonine (By similarity).
 LIPID 2 2 N-myristoyl glycine (Probable).  
Keyword
 Complete proteome; Golgi apparatus; Lipoprotein; Membrane; Myristate; Phosphoprotein; Protein transport; Reference proteome; Transport. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 446 AA 
Protein Sequence
MGLGASSEQP AGGEGFHLHG VQENSPAQQA GLEPYFDFII TIGHSRLNKE NDTLKALLKA 60
NVEKPVKLEV FNMKTMKVRE VEVVPSNMWG GQGLLGASVR FCSFRRASEH VWHVLDVEPS 120
SPAALAGLCP YTDYIVGSDQ ILQESEDFFT LIESHEGKPL KLMVYNSESD SCREVTVTPN 180
AAWGGEGSLG CGIGYGYLHR IPTQPSSQHK KPPGATPPGT PATTSQLTAF PLGAPPPWPI 240
PQDSSGPELG SRQSDFMEAL PQVPGSFMEG QLLGPGSPSH GAADCGGCLR AMEIPLQPPP 300
PVQRVMDPGF LDVSGMSLLD SSNISVCPSL SSSTVLTSTA VSVSGPEDIG SSSSSHERGG 360
EATWSGSEFE ISFPDSPGAQ AQADHLPRLT LPDGLTSAAS PEEGLSAELL EAQTEEPADT 420
ASLDCRAETE GRASQAQATP DPEPGL 446 
Gene Ontology
 GO:0005794; C:Golgi apparatus; IDA:MGI.
 GO:0016020; C:membrane; IEA:UniProtKB-KW.
 GO:0050774; P:negative regulation of dendrite morphogenesis; ISS:UniProtKB.
 GO:0015031; P:protein transport; IEA:UniProtKB-KW. 
Interpro
 IPR024958; GRASP55/65_PDZ.
 IPR007583; GRASP55_65.
 IPR001478; PDZ. 
Pfam
 PF04495; GRASP55_65 
SMART
  
PROSITE
 PS50106; PDZ 
PRINTS