CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-017754
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 WD repeat-containing protein 36 
Protein Synonyms/Alias
 T-cell activation WD repeat-containing protein; TA-WDRP 
Gene Name
 WDR36 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
235SLQLWNVKSNKLLYTubiquitination[1, 2, 3]
238LWNVKSNKLLYTFPGubiquitination[2]
284KFNETLMKFRQDWGPubiquitination[1, 2, 4, 5, 6]
322GLWDLEDKKLINQMRubiquitination[2, 3]
323LWDLEDKKLINQMRNubiquitination[2, 3]
413SFSTVHEKFNKSLGHubiquitination[1, 2, 3, 4, 6, 7]
416TVHEKFNKSLGHGLIubiquitination[2]
426GHGLINKKRVKRKGLubiquitination[2]
431NKKRVKRKGLQNTMSubiquitination[2]
446VRLPPITKFAAEEARubiquitination[1, 2, 3, 4, 6, 7]
486TIGAYFLKPKELKKDubiquitination[2]
534IHRGSFGKDQAHKGSubiquitination[2]
539FGKDQAHKGSVRGVAubiquitination[1, 2]
571KFWNFKNKILIHSVSubiquitination[2]
763LLPESRWKNLLNLDVubiquitination[2, 7, 8]
774NLDVIKKKNKPKEPPubiquitination[2]
814NNDPQQSKVVNLGVLubiquitination[1, 2, 3, 8, 9]
824NLGVLAQKSDFCLKLubiquitination[2, 8, 9]
839EEGLVNNKYDTALNLubiquitination[1, 2, 3, 8, 9]
848DTALNLLKESGPSGIubiquitination[1, 3]
887IGMMLDRKRDFELAQubiquitination[1]
Reference
 [1] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [2] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [3] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
 [4] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [5] Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass.
 Povlsen LK, Beli P, Wagner SA, Poulsen SL, Sylvestersen KB, Poulsen JW, Nielsen ML, Bekker-Jensen S, Mailand N, Choudhary C.
 Nat Cell Biol. 2012 Oct;14(10):1089-98. [PMID: 23000965]
 [6] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789]
 [7] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
 Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
 Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]
 [8] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [9] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302] 
Functional Description
 Involved in T-cell activation and highly co-regulated with IL2. 
Sequence Annotation
 REPEAT 160 197 WD 1.
 REPEAT 201 242 WD 2.
 REPEAT 287 327 WD 3.
 REPEAT 331 370 WD 4.
 REPEAT 377 417 WD 5.
 REPEAT 492 531 WD 6.
 REPEAT 537 576 WD 7.
 REPEAT 620 661 WD 8.
 REPEAT 663 701 WD 9.  
Keyword
 Complete proteome; Disease mutation; Glaucoma; Nucleus; Polymorphism; Reference proteome; Repeat; Sensory transduction; Vision; WD repeat. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 951 AA 
Protein Sequence
MCCTEGSLRK RDSQRAPEAV LCLQLWQRTV PLDTLKGLGT CFPSGPELRG AGIAAAMERA 60
SERRTASALF AGFRALGLFS NDIPHVVRFS ALKRRFYVTT CVGKSFHTYD VQKLSLVAVS 120
NSVPQDICCM AADGRLVFAA YGNVFSAFAR NKEIVHTFKG HKAEIHFLQP FGDHIISVDT 180
DGILIIWHIY SEEEYLQLTF DKSVFKISAI LHPSTYLNKI LLGSEQGSLQ LWNVKSNKLL 240
YTFPGWKVGV TALQQAPAVD VVAIGLMSGQ VIIHNIKFNE TLMKFRQDWG PITSISFRTD 300
GHPVMAAGSP CGHIGLWDLE DKKLINQMRN AHSTAIAGLT FLHREPLLVT NGADNALRIW 360
IFDGPTGEGR LLRFRMGHSA PLTNIRYYGQ NGQQILSASQ DGTLQSFSTV HEKFNKSLGH 420
GLINKKRVKR KGLQNTMSVR LPPITKFAAE EARESDWDGI IACHQGKLSC STWNYQKSTI 480
GAYFLKPKEL KKDDITATAV DITSCGNFAV IGLSSGTVDV YNMQSGIHRG SFGKDQAHKG 540
SVRGVAVDGL NQLTVTTGSE GLLKFWNFKN KILIHSVSLS SSPNIMLLHR DSGILGLALD 600
DFSISVLDIE TRKIVREFSG HQGQINDMAF SPDGRWLISA AMDCSIRTWD LPSGCLIDCF 660
LLDSAPLNVS MSPTGDFLAT SHVDHLGIYL WSNISLYSVV SLRPLPADYV PSIVMLPGTC 720
QTQDVEVSEE TVEPSDELIE YDSPEQLNEQ LVTLSLLPES RWKNLLNLDV IKKKNKPKEP 780
PKVPKSAPFF IPTIPGLVPR YAAPEQNNDP QQSKVVNLGV LAQKSDFCLK LEEGLVNNKY 840
DTALNLLKES GPSGIETELR SLSPDCGGSI EVMQSFLKMI GMMLDRKRDF ELAQAYLALF 900
LKLHLKMLPS EPVLLEEITN LSSQVEENWT HLQSLFNQSM CILNYLKSAL L 951 
Gene Ontology
 GO:0005730; C:nucleolus; IEA:UniProtKB-SubCell.
 GO:0032040; C:small-subunit processome; IEA:InterPro.
 GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
 GO:0006364; P:rRNA processing; IEA:InterPro.
 GO:0007601; P:visual perception; IEA:UniProtKB-KW. 
Interpro
 IPR011047; Quinonprotein_ADH-like_supfam.
 IPR007319; SSU_processome_Utp21.
 IPR015943; WD40/YVTN_repeat-like_dom.
 IPR001680; WD40_repeat.
 IPR019775; WD40_repeat_CS.
 IPR017986; WD40_repeat_dom. 
Pfam
 PF04192; Utp21
 PF00400; WD40 
SMART
 SM00320; WD40 
PROSITE
 PS00678; WD_REPEATS_1
 PS50082; WD_REPEATS_2
 PS50294; WD_REPEATS_REGION 
PRINTS