CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006904
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Very-long-chain 3-oxoacyl-CoA reductase 
Protein Synonyms/Alias
 3-ketoacyl-CoA reductase; 3-ketoreductase; KAR; Microsomal beta-keto-reductase 
Gene Name
 IFA38 
Gene Synonyms/Alias
 YBR159W; YBR1209 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
63KYGAKTGKYCAITGAubiquitination[1]
76GASDGIGKEFARQMAubiquitination[1]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301] 
Functional Description
 Component of the microsomal membrane bound fatty acid elongation system, which produces the 26-carbon very long-chain fatty acids (VLCFA) from palmitate. Catalyzes the reduction of the 3-ketoacyl-CoA intermediate that is formed in each cycle of fatty acid elongation. VLCFAs serve as precursors for ceramide and sphingolipids. 
Sequence Annotation
 ACT_SITE 223 223 Proton acceptor (By similarity).
 BINDING 210 210 Substrate (By similarity).  
Keyword
 Complete proteome; Endoplasmic reticulum; Fatty acid biosynthesis; Fatty acid metabolism; Lipid biosynthesis; Lipid metabolism; Membrane; NADP; Oxidoreductase; Reference proteome; Transmembrane; Transmembrane helix. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 347 AA 
Protein Sequence
MTFMQQLQEA GERFRCINGL LWVVFGLGVL KCTTLSLRFL ALIFDLFLLP AVNFDKYGAK 60
TGKYCAITGA SDGIGKEFAR QMAKRGFNLV LISRTQSKLE ALQKELEDQH HVVVKILAID 120
IAEDKESNYE SIKELCAQLP ITVLVNNVGQ SHSIPVPFLE TEEKELRNII TINNTATLLI 180
TQIIAPKIVE TVKAENKKSG TRGLILTMGS FGGLIPTPLL ATYSGSKSFL QGWSNSLAGE 240
LSKDAIDVEL IISYLVTSSM SKIRRSSLMI PNPQQFVKST LRSVGRRCGS QERYATMTPY 300
WAHAVYQFVI TETFGVYSKI VNSINYSFHK SIRIRALKKA ARQVKKE 347 
Gene Ontology
 GO:0005783; C:endoplasmic reticulum; IDA:SGD.
 GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
 GO:0016021; C:integral to membrane; ISS:SGD.
 GO:0045703; F:ketoreductase activity; IGI:SGD.
 GO:0030497; P:fatty acid elongation; IGI:SGD. 
Interpro
 IPR027533; 3_ketoreductase_fungal.
 IPR002198; DH_sc/Rdtase_SDR.
 IPR002347; Glc/ribitol_DH.
 IPR016040; NAD(P)-bd_dom.
 IPR020904; Sc_DH/Rdtase_CS. 
Pfam
 PF00106; adh_short 
SMART
  
PROSITE
 PS00061; ADH_SHORT 
PRINTS
 PR00081; GDHRDH.