UniProt Accession

 RAD24_YEAST; P32641; D3DM81 

Genbank Protein ID

 U18922; BK006939 

Genbank Nucleotide ID

 AAB64700.1; DAA07835.1 

Protein Name

 Checkpoint protein RAD24 

Protein Synonyms/Alias

  

Gene Name

 RAD24 

Gene Synonyms/Alias

 YER173W; SYGP-ORF60 

Created Date

 July 27, 2013 

Organism

 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 

NCBI Taxa ID

 559292 

Lysine Modification

Position	Peptide	Type	References
291	NMKMLKEKNKWNKRQ	acetylation	[1]

Reference

 [1] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591] 

Functional Description

 Participates in checkpoint pathways arrest of the cell cycle, a mechanism that allows the DNA repair pathways to act to restore the integrity of the DNA prior to DNA synthesis or separation of the replicated chromosomes. Regulates the DNA damage checkpoint pathway throughout the cell cycle, when associated with RCF5. Component of the RFC-like RAD24-RFC complex which loads the checkpoint clamp DDC1:MEC3:RAD17 complex and is involved in DNA repair pathways. During a clamp loading circle, the RFC:clamp complex binds to DNA and the recognition of the double- stranded/single-stranded junction stimulates ATP hydrolysis by RFC. The complex presumably provides bipartite ATP sites in which one subunit supplies a catalytic site for hydrolysis of ATP bound to the neighboring subunit. Dissociation of RFC from the clamp leaves the clamp encircling DNA. 

Sequence Annotation

 NP_BIND 109 116 ATP (Potential).
 MOD_RES 652 652 Phosphoserine.
 MOD_RES 654 654 Phosphoserine.  

Keyword

 ATP-binding; Cell cycle; Complete proteome; DNA damage; DNA repair; Nucleotide-binding; Nucleus; Phosphoprotein; Reference proteome. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 659 AA 

Protein Sequence

Gene Ontology

 GO:0005634; C:nucleus; IPI:SGD.
 GO:0031389; C:Rad17 RFC-like complex; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0000077; P:DNA damage checkpoint; IMP:SGD.
 GO:0006289; P:nucleotide-excision repair; IMP:SGD.
 GO:0007131; P:reciprocal meiotic recombination; IMP:SGD. 

Interpro

 IPR004582; Checkpoint_prot_Rad17_Rad24.
 IPR018324; Checkpoint_prot_Rad24_fun/met.
 IPR027417; P-loop_NTPase. 

Pfam

  

SMART

  

PROSITE

  

PRINTS