UniProt Accession

 CUL4A_MOUSE; Q3TCH7; Q3THM3; Q91Z44 

Genbank Protein ID

 AK168215; AK170722; BC010211 

Genbank Nucleotide ID

 BAE40173.1; BAE41979.1; AAH10211.2 

Protein Name

 Cullin-4A 

Protein Synonyms/Alias

 CUL-4A 

Gene Name

 Cul4a 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
104	KVSPTLYKQLRQVCE	ubiquitination	[1]
369	VQDLLDFKDKVDHVV	ubiquitination	[1]
371	DLLDFKDKVDHVVEV	ubiquitination	[1]
422	SKLRAGNKEATDEEL	ubiquitination	[1]
454	DVFEAFYKKDLAKRL	ubiquitination	[1]
465	AKRLLVGKSASVDAE	ubiquitination	[1]
496	SKLEGMFKDMELSKD	ubiquitination	[1]

Reference

 [1] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023] 

Functional Description

 Core component of multiple cullin-RING-based E3 ubiquitin-protein ligase complexes which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. DCX(DET1-COP1) directs ubiquitination of JUN. DCX(DDB2) directs ubiquitination of XPC. In association with RBX1, DDB1 and DDB2 is required for histone H3 and histone H4 ubiquitination in response to ultraviolet and may be important for subsequent DNA repair. DCX(DTL) plays a role in PCNA-dependent polyubiquitination of CDT1 and MDM2-dependent ubiquitination of TP53 in response to radiation-induced DNA damage and during DNA replication. In association with DDB1 and SKP2 probably is involved in ubiquitination of CDKN1B/p27kip. Is involved in ubiquitination of HOXA9 (By similarity). 

Sequence Annotation

 MOD_RES 10 10 Phosphoserine (By similarity).
 CROSSLNK 33 33 Glycyl lysine isopeptide (Lys-Gly)  

Keyword

 Complete proteome; DNA damage; DNA repair; Host-virus interaction; Isopeptide bond; Phosphoprotein; Reference proteome; Ubl conjugation; Ubl conjugation pathway. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 759 AA 

Protein Sequence

Gene Ontology

 GO:0031464; C:Cul4A-RING ubiquitin ligase complex; ISS:UniProtKB.
 GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
 GO:1900087; P:positive regulation of G1/S transition of mitotic cell cycle; IMP:MGI.
 GO:0045732; P:positive regulation of protein catabolic process; ISO:MGI.
 GO:0016567; P:protein ubiquitination; IEA:UniProtKB-UniPathway.
 GO:0006511; P:ubiquitin-dependent protein catabolic process; IEA:InterPro.
 GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW. 

Interpro

 IPR016157; Cullin_CS.
 IPR016158; Cullin_homology.
 IPR001373; Cullin_N.
 IPR019559; Cullin_neddylation_domain.
 IPR016159; Cullin_repeat-like_dom.
 IPR011991; WHTH_DNA-bd_dom. 

Pfam

 PF00888; Cullin
 PF10557; Cullin_Nedd8 

SMART

 SM00182; CULLIN
 SM00884; Cullin_Nedd8 

PROSITE

 PS01256; CULLIN_1
 PS50069; CULLIN_2 

PRINTS