CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-008602
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Probable E3 ubiquitin-protein ligase HUL5 
Protein Synonyms/Alias
  
Gene Name
 HUL5 
Gene Synonyms/Alias
 YGL141W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
24NRTRNSKKDLLEKAKacetylation[1]
31KDLLEKAKRERERRAacetylation[1]
Reference
 [1] mChIP-KAT-MS, a method to map protein interactions and acetylation sites for lysine acetyltransferases.
 Mitchell L, Huard S, Cotrut M, Pourhanifeh-Lemeri R, Steunou AL, Hamza A, Lambert JP, Zhou H, Ning Z, Basu A, Côté J, Figeys DA, Baetz K.
 Proc Natl Acad Sci U S A. 2013 Apr 23;110(17):E1641-50. [PMID: 23572591] 
Functional Description
 Probable E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Non essential. 
Sequence Annotation
 DOMAIN 810 910 HECT.
 ACT_SITE 878 878 Glycyl thioester intermediate (By  
Keyword
 Complete proteome; Ligase; Reference proteome; Ubl conjugation pathway. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 910 AA 
Protein Sequence
MLNFTGQTRR RNVNLGNRTR NSKKDLLEKA KRERERRAQD KLKEDASKTI QKSIRRHFSN 60
VRLFKNTFTS SQLVHMIPAY GGKLIYYISQ YDLQQLLKLS HNFLSSYPNS LGNRQLLSLL 120
KLYQDDALVA ETLSDLNMDC PTVDEFLDSL SVYLCRASFL SYSSASKLAD VIEAWEVMHS 180
SASISIFSIS IGSYEKRPFA LQFYCILAER NLLPQLINTN PILWDNMAKT YSHCSKGGQK 240
NIAKLLIPNF NNHIAPSVLR SDNDYVLKFY EKAFIDEVIA TTANYVSDED HVKNLMCYIA 300
SSPNQSCKNS VLITLLSNKD FVRRLSWEFF HTKFNASKTE AHPLFSVLAQ LIDMHLLIST 360
DRELLDYNSV IPIEELKKFT STLKDFTFRQ YWELPKSERN PMLKEAVPLL SKVYERDSRL 420
HFLSTENNPT YWENSEKQFL NLRFYEELQE YEDLYREHLE EESDEDMEKE IDLDKERPPL 480
KSLLLNKMKK RLKSSLRFRK LEILLELPFF IPFEERVDLF YMFIALDKKR LSLDDDHNLI 540
NMFTPWASTG MRKQSAIISR DNVLEDAFNA FNSIGERFKA SLDVTFINEF GEEAGIDGGG 600
ITKEFLTTVS DEGFKDPKHE LFRTNDRYEL YPSVVYDATK LKYIWFLGKV VGKCLYEHVL 660
IDVSFADFFL KKLLNYSNGF LSSFSDLGSY DSVLYNNLIK LLNMTTDEIK SLDLTFEIDE 720
PESSAKVVDL IPNGSKTYVT KDNVLLYVTK VTDYKLNKRC FKPVSAFHGG LSVIIAPHWM 780
EMFNSIELQM LISGERDNID LDDLKSNTEY GGYKEEDQTI VDFWEVLNEF KFEEKLNFLK 840
FVTSVPQAPL QGFKALDPKF GIRNAGTEKY RLPTASTCVN LLKLPDYRNK TILREKLLYA 900
INSGARFDLS 910 
Gene Ontology
 GO:0005737; C:cytoplasm; IDA:SGD.
 GO:0005634; C:nucleus; IDA:SGD.
 GO:0000502; C:proteasome complex; IDA:SGD.
 GO:0034450; F:ubiquitin-ubiquitin ligase activity; IDA:SGD.
 GO:0030433; P:ER-associated protein catabolic process; IMP:SGD.
 GO:0000209; P:protein polyubiquitination; IDA:SGD.
 GO:0042787; P:protein ubiquitination involved in ubiquitin-dependent protein catabolic process; IBA:RefGenome. 
Interpro
 IPR000569; HECT. 
Pfam
 PF00632; HECT 
SMART
 SM00119; HECTc 
PROSITE
 PS50237; HECT 
PRINTS