CPLM-001544

Genbank Nucleotide ID

Protein Synonyms/Alias

Homolog of ASY protein; HAP; Neuroendocrine-specific protein-like 2; NSP-like protein 2; Neuroendocrine-specific protein-like II; NSP-like protein II; NSPLII

Homo sapiens (Human)

Position	Peptide	Type	References
540	KTGEREIKEIPSCER	methylation	[1]
998	PIVYEKYKTQIDHYV	ubiquitination	[2, 3, 4]
1013	GIARDQTKSIVEKIQ	ubiquitination	[2, 4, 5]
1018	QTKSIVEKIQAKLPG	ubiquitination	[2, 3, 4, 5, 6]
1022	IVEKIQAKLPGIAKK	ubiquitination	[2, 3, 4]

[1] Large-scale global identification of protein lysine methylation in vivo.
Cao XJ, Arnaudo AM, Garcia BA.
Epigenetics. 2013 May 1;8(5):477-85. [PMID: 23644510]
[2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
[3] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
[4] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661]
[5] Integrated proteomic analysis of post-translational modifications by serial enrichment.
Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
[6] Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition.
Udeshi ND, Mani DR, Eisenhaure T, Mertins P, Jaffe JD, Clauser KR, Hacohen N, Carr SA.
Mol Cell Proteomics. 2012 May;11(5):148-59. [PMID: 22505724]

Functional Description

May be involved in membrane trafficking in the early secretory pathway. Inhibits BACE1 activity and amyloid precursor protein processing. May induce caspase-8 cascade and apoptosis. May favor BCL2 translocation to the mitochondria upon endoplasmic reticulum stress. In case of enteroviruses infection, RTN3 may be involved in the viral replication or pathogenesis.

DOMAIN 844 1032 Reticulon.
REGION 987 1032 Interaction with FADD.
REGION 1000 1002 Interaction with BACE1.
MOD_RES 2 2 N-acetylalanine.
MOD_RES 243 243 Phosphoserine (By similarity).
MOD_RES 649 649 Phosphoserine.
MOD_RES 650 650 Phosphoserine.
MOD_RES 735 735 Phosphoserine (By similarity).

Acetylation; Alternative splicing; Apoptosis; Complete proteome; Endoplasmic reticulum; ER-Golgi transport; Golgi apparatus; Host-virus interaction; Membrane; Phosphoprotein; Polymorphism; Reference proteome; Stress response; Transmembrane; Transmembrane helix; Transport.

UniProt (SWISSPROT/TrEMBL); GenBank; EMBL

GO:0005783; C:endoplasmic reticulum; IDA:HPA.
GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
GO:0005615; C:extracellular space; TAS:ProtInc.
GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
GO:0006915; P:apoptotic process; IEA:UniProtKB-KW.
GO:0071786; P:endoplasmic reticulum tubular network organization; IMP:UniProtKB.
GO:0006950; P:response to stress; IEA:UniProtKB-KW.
GO:0016192; P:vesicle-mediated transport; IEA:UniProtKB-KW.
GO:0019048; P:virus-host interaction; IEA:UniProtKB-KW.

IPR003388; Reticulon.