CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-044875
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
  
Protein Name
 AP-2 complex subunit beta 
Protein Synonyms/Alias
  
Gene Name
 AP2B1 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
5***MTDSKYFTTNKKubiquitination[1]
234KFLELLPKDSDYYNMubiquitination[2]
500STDPVTAKEVVLSEKubiquitination[3]
695AVWLPAVKAKGLEISubiquitination[1, 3]
697WLPAVKAKGLEISGTubiquitination[1, 4]
831NELQFQIKECHLNADubiquitination[1, 4]
843NADTVSSKLQNNNVYubiquitination[1]
854NNVYTIAKRNVEGQDubiquitination[1, 2, 4, 5, 6]
893PNYTLSLKCRAPEVSubiquitination[1, 3]
Reference
 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983]
 [3] A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles.
 Wagner SA, Beli P, Weinert BT, Nielsen ML, Cox J, Mann M, Choudhary C.
 Mol Cell Proteomics. 2011 Oct;10(10):M111.013284. [PMID: 21890473]
 [4] Integrated proteomic analysis of post-translational modifications by serial enrichment.
 Mertins P, Qiao JW, Patel J, Udeshi ND, Clauser KR, Mani DR, Burgess MW, Gillette MA, Jaffe JD, Carr SA.
 Nat Methods. 2013 Jul;10(7):634-7. [PMID: 23749302]
 [5] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [6] Landscape of the PARKIN-dependent ubiquitylome in response to mitochondrial depolarization.
 Sarraf SA, Raman M, Guarani-Pereira V, Sowa ME, Huttlin EL, Gygi SP, Harper JW.
 Nature. 2013 Apr 18;496(7445):372-6. [PMID: 23503661
Functional Description
  
Sequence Annotation
  
Keyword
 Complete proteome; Membrane; Reference proteome. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 913 AA 
Protein Sequence
MTDSKYFTTN KKGEIFELKA ELNNEKKEKR KEAVKKVIAA MTVGKDVSSL FPDVVNCMQT 60
DNLELKKLVY LYLMNYAKSQ PDMAIMAVNS FVKDEDPYVR KTAAVCVAKL HDINAQMVED 120
QGFLDSLRDL IADSNPMVVA NAVAALSEIS ESHPNSNLLD LNPQNINKLL TALNECTEWG 180
QIFILDCLSN YNPKDDREAQ SICERVTPRL SHANSAVVLS AVKVLMKFLE LLPKDSDYYN 240
MLLKKLAPPL VTLLSGEPEV QYVALRNINL IVQKRPEILK QEIKVFFVKY NDPIYVKLEK 300
LDIMIRLASQ ANIAQVLAEL KEYATEVDVD FVRKAVRAIG RCAIKVEQSA ERCVSTLLDL 360
IQTKVNYVVQ EAIVVIRDIF RKYPNKYESI IATLCENLDS LDEPDARAAM IWIVGEYAER 420
IDNADELLES FLEGFHDEST QVQLTLLTAI VKLFLKKPSE TQELVQQVLS LATQDSDNPD 480
LRDRGYIYWR LLSTDPVTAK EVVLSEKPLI SEETDLIEPT LLDELICHIG SLASVYHKPP 540
NAFVEGSHGI HRKHLPIHHG STDAGDSPVG TTTATNLEQP QVIPSQGDLL GDLLNLDLGP 600
PVNVPQVSSM QMGAVDLLGG GLDSLLGSDL GGGIGGSPAV GQSFIPSSVP ATFAPSPTPA 660
VVSSGLNDLF ELSTGIGMAP GGYVAPKAVW LPAVKAKGLE ISGTFTHRQG HIYMEMNFTN 720
KALQHMTDFA IQFNKNSFGV IPSTPLAIHT PLMPNQSIDV SLPLNTLGPV MKMEPLNNLQ 780
VAVKNNIDVF YFSCLIPLNV LFVEDGKMER QVFLATWKDI PNENELQFQI KECHLNADTV 840
SSKLQNNNVY TIAKRNVEGQ DMLYQSLKLT NGIWILAELR IQPGNPNYTL SLKCRAPEVS 900
QYIYQVYDSI LKN 913 
Gene Ontology
 GO:0030131; C:clathrin adaptor complex; IEA:InterPro.
 GO:0008565; F:protein transporter activity; IEA:InterPro.
 GO:0006886; P:intracellular protein transport; IEA:InterPro.
 GO:0016192; P:vesicle-mediated transport; IEA:InterPro. 
Interpro
 IPR026739; AP_beta.
 IPR016342; AP_complex_bsu_1_2_4.
 IPR011989; ARM-like.
 IPR016024; ARM-type_fold.
 IPR015151; B-adaptin_app_sub_C.
 IPR012295; Beta2_adaptin/TBP_C_dom.
 IPR002553; Clathrin/coatomer_adapt-like_N.
 IPR008152; Clathrin_a/b/g-adaptin_app_Ig.
 IPR013037; Clathrin_b-adaptin_app_Ig-like.
 IPR009028; Coatomer/calthrin_app_sub_C.
 IPR013041; Coatomer/clathrin_app_Ig-like. 
Pfam
 PF01602; Adaptin_N
 PF02883; Alpha_adaptinC2
 PF09066; B2-adapt-app_C 
SMART
 SM00809; Alpha_adaptinC2
 SM01020; B2-adapt-app_C 
PROSITE
  
PRINTS