CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-010899
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Serine/threonine-protein kinase KIN4 
Protein Synonyms/Alias
  
Gene Name
 KIN4 
Gene Synonyms/Alias
 KIN3; KIN31; YOR233W; O5220 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
66KVKLGWTKASSSNEVubiquitination[1]
359SSASSYSKSRDRNSLacetylation[2]
403KVVLNDTKNDMKESNubiquitination[1]
407NDTKNDMKESNINGEubiquitination[1]
427CRYTRDSKGNGQTQIubiquitination[1]
447RHSSRGNKHTSVAGLacetylation[2]
Reference
 [1] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, Villén J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919] 
Functional Description
 This protein is probably a serine/threonine protein kinase. 
Sequence Annotation
 DOMAIN 46 313 Protein kinase.
 NP_BIND 52 60 ATP (By similarity).
 ACT_SITE 175 175 Proton acceptor (By similarity).
 BINDING 80 80 ATP (By similarity).
 MOD_RES 365 365 Phosphoserine.
 MOD_RES 388 388 Phosphoserine.
 MOD_RES 521 521 Phosphoserine.
 MOD_RES 748 748 Phosphoserine.  
Keyword
 ATP-binding; Complete proteome; Kinase; Nucleotide-binding; Phosphoprotein; Reference proteome; Serine/threonine-protein kinase; Transferase. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 800 AA 
Protein Sequence
MASVPKRHTY GGNVVTDRDR HSLQRNNEIL HPIHKNQRKH ATFGPYIIGS TLGEGEFGKV 60
KLGWTKASSS NEVPKQVAIK LIRRDTIKKD ADKEIKIYRE INALKHLTHP NIIYLEEVLQ 120
NSKYIGIVLE FVSGGEFYKY IQRKRRLKES SACRLFAQLI SGVNYMHYKG LVHRDLKLEN 180
LLLDKHENLV ITDFGFVNEF FEDNELMKTS CGSPCYAAPE LVVSTKAYEA RKADVWSCGV 240
ILYAMLAGYL PWDDDHENPT GDDIARLYKY ITQTPLKFPE YITPIPRDLL RRILVPNPRR 300
RINLQTIKRH VWLKPHEAFL SIQPNYWDEH LQKERPKPPN KGDVGRHSTY SSSASSYSKS 360
RDRNSLIIES TLEQHRMSPQ LATSRPASPT FSTGSKVVLN DTKNDMKESN INGERTSASC 420
RYTRDSKGNG QTQIEQVSAR HSSRGNKHTS VAGLVTIPGS PTTARTRNAP SSKLTEHVKD 480
SSQTSFTQEE FHRIGNYHVP RSRPRPTSYY PGLSRNTADN SLADIPVNKL GSNGRLTDAK 540
DPVPLNAIHD TNKATISNNS IMLLSEGPAA KTSPVDYHYA IGDLNHGDKP ITEVIDKINK 600
DLTHKAAENG FPRESIDPES TSTILVTKEP TNSTDEDHVE SQLENVGHSS NKSDASSDKD 660
SKKIYEKKRF SFMSLYSSLN GSRSTVESRT SKGNAPPVSS RNPSGQSNRS NIKITQQQPR 720
NLSDRVPNPD KKINDNRIRD NAPSYAESEN PGRSVRASVM VSTLREENRS ELSNEGNNVE 780
AQTSTARKVL NFFKRRSMRV 800 
Gene Ontology
 GO:0005938; C:cell cortex; IDA:SGD.
 GO:0005935; C:cellular bud neck; IDA:SGD.
 GO:0005816; C:spindle pole body; IDA:SGD.
 GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
 GO:0004674; F:protein serine/threonine kinase activity; ISS:SGD.
 GO:0031578; P:mitotic spindle orientation checkpoint; IGI:SGD. 
Interpro
 IPR011009; Kinase-like_dom.
 IPR000719; Prot_kinase_cat_dom.
 IPR017441; Protein_kinase_ATP_BS.
 IPR002290; Ser/Thr_dual-sp_kinase_dom.
 IPR008271; Ser/Thr_kinase_AS. 
Pfam
 PF00069; Pkinase 
SMART
 SM00220; S_TKc 
PROSITE
 PS00107; PROTEIN_KINASE_ATP
 PS50011; PROTEIN_KINASE_DOM
 PS00108; PROTEIN_KINASE_ST 
PRINTS