UniProt Accession

 CTU1_HUMAN; Q7Z7A3; A8K558; Q96GZ7 

Genbank Protein ID

 AY279382; AK291173; CH471135; BC009037 

Genbank Nucleotide ID

 AAP42277.1; BAF83862.1; EAW71984.1; AAH09037.1 

Protein Name

 Cytoplasmic tRNA 2-thiolation protein 1 

Protein Synonyms/Alias

 ATP-binding domain-containing protein 3; Cancer-associated gene protein; Cytoplasmic tRNA adenylyltransferase 1 

Gene Name

 CTU1 

Gene Synonyms/Alias

 ATPBD3; NCS6 

Created Date

 July 27, 2013 

Organism

 Homo sapiens (Human) 

NCBI Taxa ID

 9606 

Lysine Modification

Position	Peptide	Type	References
319	RPRLAIGKGRRGLDE	ubiquitination	[1]
343	PARPPASKAVPTF**	ubiquitination	[1, 2]

Reference

 [1] Refined preparation and use of anti-diglycine remnant (K-ε-GG) antibody enables routine quantification of 10,000s of ubiquitination sites in single proteomics experiments.
 Udeshi ND, Svinkina T, Mertins P, Kuhn E, Mani DR, Qiao JW, Carr SA.
 Mol Cell Proteomics. 2013 Mar;12(3):825-31. [PMID: 23266961]
 [2] Systematic and quantitative assessment of the ubiquitin-modified proteome.
 Kim W, Bennett EJ, Huttlin EL, Guo A, Li J, Possemato A, Sowa ME, Rad R, Rush J, Comb MJ, Harper JW, Gygi SP.
 Mol Cell. 2011 Oct 21;44(2):325-40. [PMID: 21906983] 

Functional Description

 Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of tRNA(Lys), tRNA(Glu) and tRNA(Gln). Directly binds tRNAs and probably acts by catalyzing adenylation of tRNAs, an intermediate required for 2-thiolation. It is unclear whether it acts as a sulfurtransferase that transfers sulfur from thiocarboxylated URM1 onto the uridine of tRNAs at wobble position. 

Sequence Annotation

 MOD_RES 200 200 Phosphoserine.  

Keyword

 Complete proteome; Cytoplasm; Phosphoprotein; Polymorphism; Reference proteome; RNA-binding; Transferase; tRNA processing; tRNA-binding. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 348 AA 

Protein Sequence

Gene Ontology

 GO:0005829; C:cytosol; ISS:UniProtKB.
 GO:0005739; C:mitochondrion; IEA:Compara.
 GO:0005524; F:ATP binding; IEA:InterPro.
 GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
 GO:0000049; F:tRNA binding; IEA:UniProtKB-KW.
 GO:0034227; P:tRNA thio-modification; NAS:UniProtKB.
 GO:0002098; P:tRNA wobble uridine modification; NAS:UniProtKB. 

Interpro

 IPR012089; 2-thiocytidine_tRNA_synth_TtcA.
 IPR014729; Rossmann-like_a/b/a_fold.
 IPR011063; tRNA-lysidine/thiocyt_synth. 

Pfam

 PF01171; ATP_bind_3 

SMART

  

PROSITE

 PS01263; UPF0021 

PRINTS