UniProt Accession

 NDUA8_MOUSE; Q9DCJ5; A2AL45 

Genbank Protein ID

 AK002744; AL773525; BC012416; BC024673 

Genbank Nucleotide ID

 BAB22322.1; CAM16134.1; AAH12416.1; AAH24673.1 

Protein Name

 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 8 

Protein Synonyms/Alias

 Complex I-19kD; CI-19kD; Complex I-PGIV; CI-PGIV; NADH-ubiquinone oxidoreductase 19 kDa subunit 

Gene Name

 Ndufa8 

Gene Synonyms/Alias

  

Created Date

 July 27, 2013 

Organism

 Mus musculus (Mouse) 

NCBI Taxa ID

 10090 

Lysine Modification

Position	Peptide	Type	References
38	HYGAQCDKTNKEFML	acetylation	[1, 2]
61	RRCLKEGKLVNGCAL	acetylation	[1]
106	HCRQQQAKFDQCVLD	acetylation	[1, 2]
106	HCRQQQAKFDQCVLD	ubiquitination	[3]
159	PVIEGDLKPAKHGTR	acetylation	[1, 4]

Reference

 [1] Calorie restriction and SIRT3 trigger global reprogramming of the mitochondrial protein acetylome.
 Hebert AS, Dittenhafer-Reed KE, Yu W, Bailey DJ, Selen ES, Boersma MD, Carson JJ, Tonelli M, Balloon AJ, Higbee AJ, Westphall MS, Pagliarini DJ, Prolla TA, Assadi-Porter F, Roy S, Denu JM, Coon JJ.
 Mol Cell. 2013 Jan 10;49(1):186-99. [PMID: 23201123]
 [2] Quantification of mitochondrial acetylation dynamics highlights prominent sites of metabolic regulation.
 Still AJ, Floyd BJ, Hebert AS, Bingman CA, Carson JJ, Gunderson DR, Dolan BK, Grimsrud PA, Dittenhafer-Reed KE, Stapleton DS, Keller MP, Westphall MS, Denu JM, Attie AD, Coon JJ, Pagliarini DJ.
 J Biol Chem. 2013 Jul 17;. [PMID: 23864654]
 [3] Proteomic analyses reveal divergent ubiquitylation site patterns in murine tissues.
 Wagner SA, Beli P, Weinert BT, Schölz C, Kelstrup CD, Young C, Nielsen ML, Olsen JV, Brakebusch C, Choudhary C.
 Mol Cell Proteomics. 2012 Dec;11(12):1578-85. [PMID: 22790023]
 [4] Label-free quantitative proteomics of the lysine acetylome in mitochondria identifies substrates of SIRT3 in metabolic pathways.
 Rardin MJ, Newman JC, Held JM, Cusack MP, Sorensen DJ, Li B, Schilling B, Mooney SD, Kahn CR, Verdin E, Gibson BW.
 Proc Natl Acad Sci U S A. 2013 Apr 16;110(16):6601-6. [PMID: 23576753] 

Functional Description

 Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone (By similarity). 

Sequence Annotation

 DOMAIN 29 69 CHCH 1.
 DOMAIN 71 113 CHCH 2.
 MOTIF 36 46 C-X9-C motif 1.
 MOTIF 56 66 C-X9-C motif 2.
 MOTIF 78 88 C-X9-C motif 3.
 MOTIF 100 110 C-X9-C motif 4.
 DISULFID 36 66 Potential.
 DISULFID 46 56 Potential.
 DISULFID 78 110 Potential.
 DISULFID 88 100 Potential.  

Keyword

 Complete proteome; Direct protein sequencing; Disulfide bond; Electron transport; Mitochondrion; Reference proteome; Repeat; Respiratory chain; Transport. 

Sequence Source

 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 

Protein Length

 172 AA 

Protein Sequence

Gene Ontology

 GO:0005743; C:mitochondrial inner membrane; IDA:MGI.
 GO:0005758; C:mitochondrial intermembrane space; IEA:UniProtKB-SubCell.
 GO:0005747; C:mitochondrial respiratory chain complex I; IEA:Compara.
 GO:0022900; P:electron transport chain; IEA:UniProtKB-KW. 

Interpro

 IPR010625; CHCH.
 IPR016680; NADH_Ub_cplx-1_asu_su-8. 

Pfam

 PF06747; CHCH 

SMART

  

PROSITE

  

PRINTS