CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-006979
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 40S ribosomal protein S20 
Protein Synonyms/Alias
  
Gene Name
 RPS20 
Gene Synonyms/Alias
 URP2; YHL015W 
Created Date
 July 27, 2013 
Organism
 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) 
NCBI Taxa ID
 559292 
Lysine Modification
Position
Peptide
Type
References
6**MSDFQKEKVEEQEubiquitination[1]
8MSDFQKEKVEEQEQQacetylation[2]
8MSDFQKEKVEEQEQQubiquitination[1, 3, 4, 5, 6, 7, 8, 9, 10]
21QQQQQIIKIRITLTSacetylation[2]
21QQQQQIIKIRITLTSubiquitination[1, 10]
30RITLTSTKVKQLENVubiquitination[10]
32TLTSTKVKQLENVSSubiquitination[1, 10]
43NVSSNIVKNAEQHNLacetylation[2]
43NVSSNIVKNAEQHNLubiquitination[1, 10]
52AEQHNLVKKGPVRLPubiquitination[10]
77TPNGEGSKTWETYEMacetylation[2]
101EAPVQIVKRITQITIacetylation[2]
101EAPVQIVKRITQITIubiquitination[1, 10]
Reference
 [1] Sites of ubiquitin attachment in Saccharomyces cerevisiae.
 Starita LM, Lo RS, Eng JK, von Haller PD, Fields S.
 Proteomics. 2012 Jan;12(2):236-40. [PMID: 22106047]
 [2] Proteome-wide analysis of lysine acetylation suggests its broad regulatory scope in Saccharomyces cerevisiae.
 Henriksen P, Wagner SA, Weinert BT, Sharma S, Bacinskaja G, Rehman M, Juffer AH, Walther TC, Lisby M, Choudhary C.
 Mol Cell Proteomics. 2012 Nov;11(11):1510-22. [PMID: 22865919]
 [3] A proteomics approach to understanding protein ubiquitination.
 Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
 Nat Biotechnol. 2003 Aug;21(8):921-6. [PMID: 12872131]
 [4] A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery.
 Hitchcock AL, Auld K, Gygi SP, Silver PA.
 Proc Natl Acad Sci U S A. 2003 Oct 28;100(22):12735-40. [PMID: 14557538]
 [5] A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking.
 Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
 Mol Cell Proteomics. 2006 Apr;5(4):737-48. [PMID: 16432255]
 [6] Systematic approach for validating the ubiquitinated proteome.
 Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
 Anal Chem. 2008 Jun 1;80(11):4161-9. [PMID: 18433149]
 [7] Computational identification of ubiquitylation sites from protein sequences.
 Tung CW, Ho SY.
 BMC Bioinformatics. 2008 Jul 15;9:310. [PMID: 18625080]
 [8] Identification, analysis, and prediction of protein ubiquitination sites.
 Radivojac P, Vacic V, Haynes C, Cocklin RR, Mohan A, Heyen JW, Goebl MG, Iakoucheva LM.
 Proteins. 2010 Feb 1;78(2):365-80. [PMID: 19722269]
 [9] A perturbed ubiquitin landscape distinguishes between ubiquitin in trafficking and in proteolysis.
 Ziv I, Matiuhin Y, Kirkpatrick DS, Erpapazoglou Z, Leon S, Pantazopoulou M, Kim W, Gygi SP, Haguenauer-Tsapis R, Reis N, Glickman MH, Kleifeld O.
 Mol Cell Proteomics. 2011 May;10(5):M111.009753. [PMID: 21427232]
 [10] Global analysis of phosphorylation and ubiquitylation cross-talk in protein degradation.
 Swaney DL, Beltrao P, Starita L, Guo A, Rush J, Fields S, Krogan NJ, VillĂ©n J.
 Nat Methods. 2013 Jul;10(7):676-82. [PMID: 23749301
Functional Description
  
Sequence Annotation
 MOD_RES 2 2 N-acetylserine.
 CROSSLNK 8 8 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 3D-structure; Acetylation; Complete proteome; Cytoplasm; Direct protein sequencing; Isopeptide bond; Reference proteome; Ribonucleoprotein; Ribosomal protein; Ubl conjugation. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 121 AA 
Protein Sequence
MSDFQKEKVE EQEQQQQQII KIRITLTSTK VKQLENVSSN IVKNAEQHNL VKKGPVRLPT 60
KVLKISTRKT PNGEGSKTWE TYEMRIHKRY IDLEAPVQIV KRITQITIEP GVDVEVVVAS 120
N 121 
Gene Ontology
 GO:0022627; C:cytosolic small ribosomal subunit; IDA:SGD.
 GO:0003723; F:RNA binding; IEA:InterPro.
 GO:0003735; F:structural constituent of ribosome; IC:SGD.
 GO:0002181; P:cytoplasmic translation; IC:SGD.
 GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IMP:SGD. 
Interpro
 IPR001848; Ribosomal_S10.
 IPR018268; Ribosomal_S10_CS.
 IPR027486; Ribosomal_S10_dom.
 IPR005729; Ribosomal_S10_euk/arc. 
Pfam
 PF00338; Ribosomal_S10 
SMART
  
PROSITE
 PS00361; RIBOSOMAL_S10 
PRINTS
 PR00971; RIBOSOMALS10.