CPLM 1.0 - Compendium of Protein Lysine Modification
TagContent
CPLM ID CPLM-003762
UniProt Accession
Genbank Protein ID
Genbank Nucleotide ID
Protein Name
 Transcriptional activator GLI3 
Protein Synonyms/Alias
 GLI3 form of 190 kDa; GLI3-190; GLI3 full length protein; GLI3FL; Transcriptional repressor GLI3R; GLI3 C-terminally truncated form; GLI3 form of 83 kDa; GLI3-83 
Gene Name
 GLI3 
Gene Synonyms/Alias
  
Created Date
 July 27, 2013 
Organism
 Homo sapiens (Human) 
NCBI Taxa ID
 9606 
Lysine Modification
Position
Peptide
Type
References
87DERASLIKKEIHGSLsumoylation[1]
462PEGTTLVKEEGDKDEsumoylation[1]
640GPEAHVTKKQRGDIHubiquitination[2]
696CLQVKTVKAEKPMTSsumoylation[1]
773RRNPAGTKWMEHVKLubiquitination[3]
779TKWMEHVKLERLKQVsumoylation[1]
779TKWMEHVKLERLKQVubiquitination[3]
784HVKLERLKQVNGMFPubiquitination[3]
800LNPILPPKAPAVSPLubiquitination[3]
1422TCRVNGIKMEMKGQPsumoylation[1]
Reference
 [1] SUMOylation by Pias1 regulates the activity of the Hedgehog dependent Gli transcription factors.
 Cox B, Briscoe J, Ulloa F.
 PLoS One. 2010 Aug 11;5(8):e11996. [PMID: 20711444]
 [2] Global identification of modular cullin-RING ligase substrates.
 Emanuele MJ, Elia AE, Xu Q, Thoma CR, Izhar L, Leng Y, Guo A, Chen YN, Rush J, Hsu PW, Yen HC, Elledge SJ.
 Cell. 2011 Oct 14;147(2):459-74. [PMID: 21963094]
 [3] hCKSAAP_UbSite: improved prediction of human ubiquitination sites by exploiting amino acid pattern and properties.
 Chen Z, Zhou Y, Song J, Zhang Z.
 Biochim Biophys Acta. 2013 Aug;1834(8):1461-7. [PMID: 23603789] 
Functional Description
 Has a dual function as a transcriptional activator and a repressor of the sonic hedgehog (Shh) pathway, and plays a role in limb development. The full-length GLI3 form (GLI3FL) after phosphorylation and nuclear translocation, acts as an activator (GLI3A) while GLI3R, its C-terminally truncated form, acts as a repressor. A proper balance between the GLI3 activator and the repressor GLI3R, rather than the repressor gradient itself or the activator/repressor ratio gradient, specifies limb digit number and identity. In concert with TRPS1, plays a role in regulating the size of the zone of distal chondrocytes, in restricting the zone of PTHLH expression in distal cells and in activating chondrocyte proliferation. Binds to the minimal GLI-consensus sequence 5'-GGGTGGTC-3'. 
Sequence Annotation
 ZN_FING 480 505 C2H2-type 1.
 ZN_FING 513 540 C2H2-type 2.
 ZN_FING 546 570 C2H2-type 3.
 ZN_FING 576 601 C2H2-type 4.
 ZN_FING 607 632 C2H2-type 5.
 MOD_RES 1 1 N-acetylmethionine.
 MOD_RES 664 664 Phosphoserine.
 MOD_RES 849 849 Phosphoserine; by PKA.
 MOD_RES 865 865 Phosphoserine; by PKA.
 MOD_RES 877 877 Phosphoserine; by PKA.
 MOD_RES 907 907 Phosphoserine; by PKA.
 MOD_RES 980 980 Phosphoserine; by PKA.
 MOD_RES 1006 1006 Phosphoserine; by PKA.
 CROSSLNK 773 773 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 779 779 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 784 784 Glycyl lysine isopeptide (Lys-Gly)
 CROSSLNK 800 800 Glycyl lysine isopeptide (Lys-Gly)  
Keyword
 Acetylation; Activator; Cell projection; Cilium; Complete proteome; Cytoplasm; Disease mutation; DNA-binding; Isopeptide bond; Metal-binding; Nucleus; Phosphoprotein; Polymorphism; Reference proteome; Repeat; Repressor; Transcription; Transcription regulation; Ubl conjugation; Zinc; Zinc-finger. 
Sequence Source
 UniProt (SWISSPROT/TrEMBL); GenBank; EMBL 
Protein Length
 1580 AA 
Protein Sequence
MEAQSHSSTT TEKKKVENSI VKCSTRTDVS EKAVASSTTS NEDESPGQTY HRERRNAITM 60
QPQNVQGLSK VSEEPSTSSD ERASLIKKEI HGSLPHVAEP SVPYRGTVFA MDPRNGYMEP 120
HYHPPHLFPA FHPPVPIDAR HHEGRYHYDP SPIPPLHMTS ALSSSPTYPD LPFIRISPHR 180
NPTAASESPF SPPHPYINPY MDYIRSLHSS PSLSMISATR GLSPTDAPHA GVSPAEYYHQ 240
MALLTGQRSP YADIIPSAAT AGTGAIHMEY LHAMDSTRFS SPRLSARPSR KRTLSISPLS 300
DHSFDLQTMI RTSPNSLVTI LNNSRSSSSA SGSYGHLSAS AISPALSFTY SSAPVSLHMH 360
QQILSRQQSL GSAFGHSPPL IHPAPTFPTQ RPIPGIPTVL NPVQVSSGPS ESSQNKPTSE 420
SAVSSTGDPM HNKRSKIKPD EDLPSPGARG QQEQPEGTTL VKEEGDKDES KQEPEVIYET 480
NCHWEGCARE FDTQEQLVHH INNDHIHGEK KEFVCRWLDC SREQKPFKAQ YMLVVHMRRH 540
TGEKPHKCTF EGCTKAYSRL ENLKTHLRSH TGEKPYVCEH EGCNKAFSNA SDRAKHQNRT 600
HSNEKPYVCK IPGCTKRYTD PSSLRKHVKT VHGPEAHVTK KQRGDIHPRP PPPRDSGSHS 660
QSRSPGRPTQ GALGEQQDLS NTTSKREECL QVKTVKAEKP MTSQPSPGGQ SSCSSQQSPI 720
SNYSNSGLEL PLTDGGSIGD LSAIDETPIM DSTISTATTA LALQARRNPA GTKWMEHVKL 780
ERLKQVNGMF PRLNPILPPK APAVSPLIGN GTQSNNTCSL GGPMTLLPGR SDLSGVDVTM 840
LNMLNRRDSS ASTISSAYLS SRRSSGISPC FSSRRSSEAS QAEGRPQNVS VADSYDPIST 900
DASRRSSEAS QSDGLPSLLS LTPAQQYRLK AKYAAATGGP PPTPLPNMER MSLKTRLALL 960
GDALEPGVAL PPVHAPRRCS DGGAHGYGRR HLQPHDAPGH GVRRASDPVR TGSEGLALPR 1020
VPRFSSLSSC NPPAMATSAE KRSLVLQNYT RPEGGQSRNF HSSPCPPSIT ENVTLESLTM 1080
DADANLNDED FLPDDVVQYL NSQNQAGYEQ HFPSALPDDS KVPHGPGDFD APGLPDSHAG 1140
QQFHALEQPC PEGSKTDLPI QWNEVSSGSA DLSSSKLKCG PRPAVPQTRA FGFCNGMVVH 1200
PQNPLRSGPA GGYQTLGENS NPYGGPEHLM LHNSPGSGTS GNAFHEQPCK APQYGNCLNR 1260
QPVAPGALDG ACGAGIQASK LKSTPMQGSG GQLNFGLPVA PNESAGSMVN GMQNQDPVGQ 1320
GYLAHQLLGD SMQHPGAGRP GQQMLGQISA TSHINIYQGP ESCLPGAHGM GSQPSSLAVV 1380
RGYQPCASFG GSRRQAMPRD SLALQSGQLS DTSQTCRVNG IKMEMKGQPH PLCSNLQNYS 1440
GQFYDQTVGF SQQDTKAGSF SISDASCLLQ GTSAKNSELL SPGANQVTST VDSLDSHDLE 1500
GVQIDFDAII DDGDHSSLMS GALSPSIIQN LSHSSSRLTT PRASLPFPAL SMSTTNMAIG 1560
DMSSLLTSLA EESKFLAVMQ 1580 
Gene Ontology
 GO:0005929; C:cilium; IDA:UniProtKB.
 GO:0005829; C:cytosol; IDA:UniProtKB.
 GO:0005730; C:nucleolus; IDA:HPA.
 GO:0017053; C:transcriptional repressor complex; IEA:Compara.
 GO:0003682; F:chromatin binding; IEA:Compara.
 GO:0042826; F:histone deacetylase binding; IDA:UniProtKB.
 GO:0043565; F:sequence-specific DNA binding; IEA:Compara.
 GO:0003700; F:sequence-specific DNA binding transcription factor activity; IDA:UniProtKB.
 GO:0008270; F:zinc ion binding; IEA:InterPro.
 GO:0060873; P:anterior semicircular canal development; IEA:Compara.
 GO:0009952; P:anterior/posterior pattern specification; IEA:Compara.
 GO:0060840; P:artery development; IEA:Compara.
 GO:0007411; P:axon guidance; IEA:Compara.
 GO:0001658; P:branching involved in ureteric bud morphogenesis; IEA:Compara.
 GO:0048593; P:camera-type eye morphogenesis; IEA:Compara.
 GO:0061005; P:cell differentiation involved in kidney development; IEA:Compara.
 GO:0021801; P:cerebral cortex radial glia guided migration; IEA:Compara.
 GO:0048589; P:developmental growth; IEA:Compara.
 GO:0048566; P:embryonic digestive tract development; TAS:BHF-UCL.
 GO:0048557; P:embryonic digestive tract morphogenesis; IEA:Compara.
 GO:0042733; P:embryonic digit morphogenesis; TAS:BHF-UCL.
 GO:0048704; P:embryonic skeletal system morphogenesis; IEA:Compara.
 GO:0021798; P:forebrain dorsal/ventral pattern formation; IEA:Compara.
 GO:0021861; P:forebrain radial glial cell differentiation; IEA:Compara.
 GO:0060364; P:frontal suture morphogenesis; IEA:Compara.
 GO:0007507; P:heart development; IEA:Compara.
 GO:0007442; P:hindgut morphogenesis; IEA:Compara.
 GO:0001701; P:in utero embryonic development; IEA:Compara.
 GO:0060366; P:lambdoid suture morphogenesis; IEA:Compara.
 GO:0022018; P:lateral ganglionic eminence cell proliferation; IEA:Compara.
 GO:0060875; P:lateral semicircular canal development; IEA:Compara.
 GO:0030324; P:lung development; IEA:Compara.
 GO:0060594; P:mammary gland specification; IEA:Compara.
 GO:0030318; P:melanocyte differentiation; IEA:Compara.
 GO:0001656; P:metanephros development; IEA:Compara.
 GO:0046639; P:negative regulation of alpha-beta T cell differentiation; ISS:BHF-UCL.
 GO:0043066; P:negative regulation of apoptotic process; IEA:Compara.
 GO:0090090; P:negative regulation of canonical Wnt receptor signaling pathway; IDA:UniProtKB.
 GO:0008285; P:negative regulation of cell proliferation; IEA:Compara.
 GO:0045665; P:negative regulation of neuron differentiation; IEA:Compara.
 GO:0045879; P:negative regulation of smoothened signaling pathway; ISS:BHF-UCL.
 GO:0000122; P:negative regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0045060; P:negative thymic T cell selection; ISS:BHF-UCL.
 GO:0043585; P:nose morphogenesis; TAS:BHF-UCL.
 GO:0042475; P:odontogenesis of dentin-containing tooth; IEA:Compara.
 GO:0048709; P:oligodendrocyte differentiation; IEA:Compara.
 GO:0021631; P:optic nerve morphogenesis; IEA:Compara.
 GO:0060021; P:palate development; IEA:Compara.
 GO:0046638; P:positive regulation of alpha-beta T cell differentiation; ISS:BHF-UCL.
 GO:0032332; P:positive regulation of chondrocyte differentiation; IEA:Compara.
 GO:0002052; P:positive regulation of neuroblast proliferation; IEA:Compara.
 GO:0045669; P:positive regulation of osteoblast differentiation; IEA:Compara.
 GO:0042307; P:positive regulation of protein import into nucleus; IEA:Compara.
 GO:0045944; P:positive regulation of transcription from RNA polymerase II promoter; IDA:UniProtKB.
 GO:0016485; P:protein processing; IEA:Compara.
 GO:0009954; P:proximal/distal pattern formation; IEA:Compara.
 GO:0060367; P:sagittal suture morphogenesis; IEA:Compara.
 GO:0007224; P:smoothened signaling pathway; TAS:BHF-UCL.
 GO:0060831; P:smoothened signaling pathway involved in dorsal/ventral neural tube patterning; IEA:Compara.
 GO:0021776; P:smoothened signaling pathway involved in spinal cord motor neuron cell fate specification; IEA:Compara.
 GO:0021775; P:smoothened signaling pathway involved in ventral spinal cord interneuron specification; IEA:Compara.
 GO:0070242; P:thymocyte apoptotic process; ISS:BHF-UCL.
 GO:0043586; P:tongue development; IEA:Compara.
 GO:0006351; P:transcription, DNA-dependent; IEA:UniProtKB-KW. 
Interpro
 IPR007087; Znf_C2H2.
 IPR015880; Znf_C2H2-like.
 IPR013087; Znf_C2H2/integrase_DNA-bd. 
Pfam
 PF00096; zf-C2H2 
SMART
 SM00355; ZnF_C2H2 
PROSITE
 PS00028; ZINC_FINGER_C2H2_1
 PS50157; ZINC_FINGER_C2H2_2 
PRINTS